(Linear) (Six-base) MAPSORT of: KIEE4233.seq Check: 7277 from: 1 to: 2550 KIEE4233.orf With 182 enzymes: SgfI * March 22, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2218 2550 Size: 2218 332 AccI GT'mk_AC Cuts at: 0 1379 2550 Size: 1379 1171 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 950 2550 Size: 950 1600 AflII C'TTAA_G Cuts at: 0 2311 2550 Size: 2311 239 AhdI GACnn_n'nnGTC Cuts at: 0 918 2550 Size: 918 1632 AleI CACnn'nnGTG Cuts at: 0 1138 2550 Size: 1138 1412 AlwNI CAG_nnn'CTG Cuts at: 0 117 2550 Size: 117 2433 ApaI G_GGCC'C Cuts at: 0 604 2550 Size: 604 1946 ApaLI G'TGCA_C Cuts at: 0 1810 2550 Size: 1810 740 ApoI r'AATT_y Cuts at: 0 144 198 268 1194 1702 1763 1872 2282 Size: 144 54 70 926 508 61 109 410 Cuts at: 2282 2550 Size: 268 Fragments arranged by size: 926 508 410 268 144 109 70 61 54 AvaI C'yCGr_G Cuts at: 0 70 2550 Size: 70 2480 AvrII C'CTAG_G Cuts at: 0 804 2550 Size: 804 1746 BanI G'GyrC_C Cuts at: 0 12 42 2550 Size: 12 30 2508 Fragments arranged by size: 2508 30 12 BanII G_rGCy'C Cuts at: 0 71 604 1845 1913 2002 2550 Size: 71 533 1241 68 89 548 Fragments arranged by size: 1241 548 533 89 71 68 BbeI G_GCGC'C Cuts at: 0 16 46 2550 Size: 16 30 2504 Fragments arranged by size: 2504 30 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 383 631 956 2270 2550 Size: 383 248 325 1314 280 Fragments arranged by size: 1314 383 325 280 248 BbvCI CC'TCA_GC Cuts at: 0 1032 2550 Size: 1032 1518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1874 1908 2550 Size: 1874 34 642 Fragments arranged by size: 1874 642 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1155 2550 Size: 1155 1395 BglI GCCn_nnn'nGGC Cuts at: 0 122 2550 Size: 122 2428 BglII A'GATC_T Cuts at: 0 128 342 2550 Size: 128 214 2208 Fragments arranged by size: 2208 214 128 Bme1580I G_kGCm'C Cuts at: 0 604 1247 1814 2550 Size: 604 643 567 736 Fragments arranged by size: 736 643 604 567 BmrI ACTGGGnnnn_n' Cuts at: 0 711 1954 2550 Size: 711 1243 596 Fragments arranged by size: 1243 711 596 Bpu10I CC'TnA_GC Cuts at: 0 1032 2550 Size: 1032 1518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1829 2550 Size: 1829 721 BsaI GGTCTCn'nnnn_ Cuts at: 0 926 2550 Size: 926 1624 BsaAI yAC'GTr Cuts at: 0 957 2550 Size: 957 1593 BsaHI Gr'CG_yC Cuts at: 0 13 43 2550 Size: 13 30 2507 Fragments arranged by size: 2507 30 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1328 1358 2550 Size: 1328 30 1192 Fragments arranged by size: 1328 1192 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 748 1284 2550 Size: 748 536 1266 Fragments arranged by size: 1266 748 536 BseYI C'CCAG_C Cuts at: 0 1649 2550 Size: 1649 901 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1090 2550 Size: 1090 1460 BsiHKAI G_wGCw'C Cuts at: 0 1814 1845 1913 2002 2550 Size: 1814 31 68 89 548 Fragments arranged by size: 1814 548 89 68 31 Bsp1286I G_dGCh'C Cuts at: 0 71 604 1247 1814 1845 1913 2002 2550 Size: 71 533 643 567 31 68 89 548 Fragments arranged by size: 643 567 548 533 89 71 68 31 BspHI T'CATG_A Cuts at: 0 2472 2550 Size: 2472 78 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2218 2550 Size: 2218 332 BsrGI T'GTAC_A Cuts at: 0 522 2550 Size: 522 2028 BstAPI GCAn_nnn'nTGC Cuts at: 0 117 2550 Size: 117 2433 BstEII G'GTnAC_C Cuts at: 0 684 2550 Size: 684 1866 BstXI CCAn_nnnn'nTGG Cuts at: 0 201 1140 2550 Size: 201 939 1410 Fragments arranged by size: 1410 939 201 BstYI r'GATC_y Cuts at: 0 128 342 691 2494 2550 Size: 128 214 349 1803 56 Fragments arranged by size: 1803 349 214 128 56 BtgI C'CryG_G Cuts at: 0 540 2550 Size: 540 2010 BtsI GCAGTG_nn' Cuts at: 0 1133 1514 2550 Size: 1133 381 1036 Fragments arranged by size: 1133 1036 381 ClaI AT'CG_AT Cuts at: 0 1313 2550 Size: 1313 1237 EaeI y'GGCC_r Cuts at: 0 561 2550 Size: 561 1989 EarI CTCTTCn'nnn_ Cuts at: 0 725 929 1988 2324 2550 Size: 725 204 1059 336 226 Fragments arranged by size: 1059 725 336 226 204 EcoICRI GAG'CTC Cuts at: 0 1843 1911 2000 2550 Size: 1843 68 89 550 Fragments arranged by size: 1843 550 89 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 950 2550 Size: 950 1600 EcoO109I rG'GnC_Cy Cuts at: 0 601 2550 Size: 601 1949 EcoRV GAT'ATC Cuts at: 0 1440 2550 Size: 1440 1110 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 478 2550 Size: 478 2072 HaeII r_GCGC'y Cuts at: 0 16 46 2550 Size: 16 30 2504 Fragments arranged by size: 2504 30 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 904 936 1452 1484 2550 Size: 904 32 516 32 1066 Fragments arranged by size: 1066 904 516 32 32 HincII GTy'rAC Cuts at: 0 429 2550 Size: 429 2121 KasI G'GCGC_C Cuts at: 0 12 42 2550 Size: 12 30 2508 Fragments arranged by size: 2508 30 12 MfeI C'AATT_G Cuts at: 0 1397 2531 2550 Size: 1397 1134 19 Fragments arranged by size: 1397 1134 19 MscI TGG'CCA Cuts at: 0 563 2550 Size: 563 1987 MslI CAynn'nnrTG Cuts at: 0 1138 1429 1589 1695 2550 Size: 1138 291 160 106 855 Fragments arranged by size: 1138 855 291 160 106 MspA1I CmG'CkG Cuts at: 0 49 1036 2550 Size: 49 987 1514 Fragments arranged by size: 1514 987 49 NarI GG'CG_CC Cuts at: 0 13 43 2550 Size: 13 30 2507 Fragments arranged by size: 2507 30 13 NcoI C'CATG_G Cuts at: 0 540 2550 Size: 540 2010 NruI TCG'CGA Cuts at: 0 95 2550 Size: 95 2455 NspI r_CATG'y Cuts at: 0 539 2550 Size: 539 2011 PflMI CCAn_nnn'nTGG Cuts at: 0 160 234 2536 2550 Size: 160 74 2302 14 Fragments arranged by size: 2302 160 74 14 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1599 1631 2550 Size: 1599 32 919 Fragments arranged by size: 1599 919 32 PspOMI G'GGCC_C Cuts at: 0 600 2550 Size: 600 1950 PstI C_TGCA'G Cuts at: 0 66 1509 2107 2229 2241 2321 2550 Size: 66 1443 598 122 12 80 229 Fragments arranged by size: 1443 598 229 122 80 66 12 PvuII CAG'CTG Cuts at: 0 1036 2550 Size: 1036 1514 SacI G_AGCT'C Cuts at: 0 1845 1913 2002 2550 Size: 1845 68 89 548 Fragments arranged by size: 1845 548 89 68 ScaI AGT'ACT Cuts at: 0 973 2550 Size: 973 1577 SfcI C'TryA_G Cuts at: 0 62 871 1505 1541 2103 2225 2237 2317 Size: 62 809 634 36 562 122 12 80 Cuts at: 2317 2550 Size: 233 Fragments arranged by size: 809 634 562 233 122 80 62 36 12 SfoI GGC'GCC Cuts at: 0 14 44 2550 Size: 14 30 2506 Fragments arranged by size: 2506 30 14 SmlI C'TyrA_G Cuts at: 0 1844 2311 2550 Size: 1844 467 239 Fragments arranged by size: 1844 467 239 SphI G_CATG'C Cuts at: 0 539 2550 Size: 539 2011 StyI C'CwwG_G Cuts at: 0 540 804 2550 Size: 540 264 1746 Fragments arranged by size: 1746 540 264 TatI w'GTAC_w Cuts at: 0 522 971 2221 2550 Size: 522 449 1250 329 Fragments arranged by size: 1250 522 449 329 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2039 2071 2550 Size: 2039 32 479 Fragments arranged by size: 2039 479 32 XmnI GAAnn'nnTTC Cuts at: 0 843 2286 2550 Size: 843 1443 264 Fragments arranged by size: 1443 843 264 Enzymes that do cut: AarI AccI AcuI AflII AhdI AleI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII Bme1580I BmrI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MfeI MscI MslI MspA1I NarI NcoI NruI NspI PflMI PpiI PspOMI PstI PvuII SacI ScaI SfcI SfoI SmlI SphI StyI TatI TstI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AlfI AloI AscI AseI AsiSI BaeI BamHI BclI BfrBI BlpI BmgBI BmtI BplI BpmI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KpnI MluI MmeI NaeI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI