(Circular) (Six-base) MAPSORT of: pFN21AE0985.seq Check: 7608 from: 1 to: 6493 pFN21AE0985 6493 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2443 2443 Size: 6493 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 2195 3814 2195 Size: 1619 4874 AclI AA'CG_TT Cuts at: 1563 3483 4620 4993 1563 Size: 1920 1137 373 3063 Fragments arranged by size: 3063 1920 1137 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 3341 4556 5940 6208 1632 Size: 542 1167 1215 1384 268 1917 Fragments arranged by size: 1917 1384 1215 1167 542 268 AfeI AGC'GCT Cuts at: 3736 3736 Size: 6493 AflII C'TTAA_G Cuts at: 820 1017 3665 3683 820 Size: 197 2648 18 3630 Fragments arranged by size: 3630 2648 197 18 AflIII A'CryG_T Cuts at: 1203 3396 5392 6369 1203 Size: 2193 1996 977 1327 Fragments arranged by size: 2193 1996 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 3155 1166 Size: 1989 4504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6493 AlwNI CAG_nnn'CTG Cuts at: 4236 5808 4236 Size: 1572 4921 ApaI G_GGCC'C Cuts at: 2262 2283 3586 2262 Size: 21 1303 5169 Fragments arranged by size: 5169 1303 21 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 2037 2849 3802 4028 4180 6289 1493 Size: 544 812 953 226 152 2109 1697 Fragments arranged by size: 2109 1697 953 812 544 226 152 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2677 3766 3818 1870 Size: 73 734 1089 52 4545 Fragments arranged by size: 4545 1089 734 73 52 BamHI G'GATC_C Cuts at: 2140 2368 2563 3183 3823 4190 2140 Size: 228 195 620 640 367 4443 Fragments arranged by size: 4443 640 620 367 228 195 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2195 2539 3814 5269 Size: 325 217 627 275 133 344 1275 1455 Cuts at: 5269 618 Size: 1842 Fragments arranged by size: 1842 1455 1275 627 344 325 275 217 133 BanII G_rGCy'C Cuts at: 1391 2262 2283 2432 2526 3105 3586 3812 1391 Size: 871 21 149 94 579 481 226 4072 Fragments arranged by size: 4072 871 579 481 226 149 94 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5522 BbvCI CC'TCA_GC Cuts at: 2825 2825 Size: 6493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4684 4718 1903 Size: 34 2747 34 3678 Fragments arranged by size: 3678 2747 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 3414 1548 Size: 1866 4627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2649 5104 136 Size: 107 122 71 631 605 977 2455 1525 Fragments arranged by size: 2455 1525 977 631 605 122 107 71 BglII A'GATC_T Cuts at: 3171 3675 6488 3171 Size: 504 2813 3176 Fragments arranged by size: 3176 2813 504 BlpI GC'TnA_GC Cuts at: 2343 3431 3910 2343 Size: 1088 479 4926 Fragments arranged by size: 4926 1088 479 Bme1580I G_kGCm'C Cuts at: 1790 2262 2283 2544 3586 3775 4554 5710 1790 Size: 472 21 261 1042 189 779 1156 2573 Fragments arranged by size: 2573 1156 1042 779 472 261 189 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3595 4224 5182 Size: 416 53 83 399 24 2178 629 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 2178 1753 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2458 3011 5153 1239 Size: 1219 553 2142 2579 Fragments arranged by size: 2579 2142 1219 553 Bpu10I CC'TnA_GC Cuts at: 2825 2825 Size: 6493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 3209 4618 5483 5781 2915 Size: 294 1409 865 298 3627 Fragments arranged by size: 3627 1409 865 298 294 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2312 3464 3509 5156 882 Size: 617 813 1152 45 1647 2219 Fragments arranged by size: 2219 1647 1152 813 617 45 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3661 4300 4682 5317 Size: 53 83 186 936 2128 639 382 635 Cuts at: 5317 275 Size: 1451 Fragments arranged by size: 2128 1451 936 639 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2137 4926 5324 5598 5745 6156 2137 Size: 2789 398 274 147 411 2474 Fragments arranged by size: 2789 2474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3365 3395 3539 3569 1178 Size: 30 165 30 1962 30 144 30 4102 Fragments arranged by size: 4102 1962 165 144 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 1186 Size: 1582 4911 BseYI C'CCAG_C Cuts at: 2032 2276 2298 2453 5696 6192 2032 Size: 244 22 155 3243 496 2333 Fragments arranged by size: 3243 2333 496 244 155 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5920 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3943 4704 5732 1540 Size: 275 186 1942 761 1028 2301 Fragments arranged by size: 2301 1942 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2526 3105 4554 4639 5710 2526 Size: 579 1449 85 1071 3309 Fragments arranged by size: 3309 1449 1071 579 85 BsmI GAATG_Cn' Cuts at: 4004 4097 4004 Size: 93 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2283 2432 2526 2544 3105 3586 Size: 399 472 21 149 94 18 561 481 Cuts at: 3586 3775 3812 4554 4639 5710 1391 Size: 189 37 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 481 472 399 189 149 94 85 37 21 18 BspEI T'CCGG_A Cuts at: 2137 6156 2137 Size: 4019 2474 BspHI T'CATG_A Cuts at: 3003 3696 4382 6112 3003 Size: 693 686 1730 3384 Fragments arranged by size: 3384 1730 693 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2443 2915 3848 844 Size: 1599 472 933 3489 Fragments arranged by size: 3489 1599 933 472 BsrBI CCG'CTC Cuts at: 1394 1823 3435 4380 1394 Size: 429 1612 945 3507 Fragments arranged by size: 3507 1612 945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3723 4988 5162 65 Size: 1183 2475 1265 174 1396 Fragments arranged by size: 2475 1396 1265 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5137 5324 1700 Size: 110 3327 187 2869 Fragments arranged by size: 3327 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6493 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstXI CCAn_nnnn'nTGG Cuts at: 2372 2372 Size: 6493 BstYI r'GATC_y Cuts at: 1972 2140 2368 2563 3171 3183 3675 3823 4190 Size: 168 228 195 608 12 492 148 367 Cuts at: 4190 4575 4592 6044 6130 6142 6488 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 608 492 385 367 346 228 195 168 148 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2356 3357 2356 Size: 1001 5492 BtgI C'CryG_G Cuts at: 513 1065 2004 2262 2559 3153 513 Size: 552 939 258 297 594 3853 Fragments arranged by size: 3853 939 594 552 297 258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 2278 2851 4009 4803 4823 2278 Size: 573 1158 794 20 3948 Fragments arranged by size: 3948 1158 794 573 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 2650 3940 Size: 54 1425 50 275 18 663 157 1290 Cuts at: 3940 4829 6458 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1290 889 663 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3940 1537 Size: 275 2128 4090 Fragments arranged by size: 4090 2128 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3110 3194 3507 3695 4423 1353 Size: 1757 84 313 188 728 3423 Fragments arranged by size: 3423 1757 728 313 188 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3111 3650 5080 5454 5600 387 Size: 1064 138 1522 539 1430 374 146 1280 Fragments arranged by size: 1522 1430 1280 1064 539 374 146 138 EcoICRI GAG'CTC Cuts at: 2524 3103 2524 Size: 579 5914 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2458 3011 3341 4556 5153 5940 Size: 393 542 284 553 330 1215 597 787 Cuts at: 5940 6208 1239 Size: 268 1524 Fragments arranged by size: 1524 1215 787 597 553 542 393 330 284 268 EcoO109I rG'GnC_Cy Cuts at: 2072 2228 2258 2280 2731 3336 3582 2072 Size: 156 30 22 451 605 246 4983 Fragments arranged by size: 4983 605 451 246 156 30 22 EcoRI G'AATT_C Cuts at: 2037 2849 3802 4180 2037 Size: 812 953 378 4350 Fragments arranged by size: 4350 953 812 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3346 3736 3346 Size: 390 6103 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 2876 3709 3738 5640 1678 Size: 1198 833 29 1902 2531 Fragments arranged by size: 2531 1902 1198 833 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3335 3365 3367 3397 3515 Size: 32 135 32 1763 30 2 30 118 Cuts at: 3515 3539 3547 3571 5134 5166 5208 5240 1373 Size: 24 8 24 1563 32 42 32 2626 Fragments arranged by size: 2626 1763 1563 135 118 42 32 32 32 32 30 30 24 24 8 2 HincII GTy'rAC Cuts at: 669 1657 1938 3250 3837 4083 669 Size: 988 281 1312 587 246 3079 Fragments arranged by size: 3079 1312 988 587 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 2199 3818 2199 Size: 1619 4874 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3582 5607 5791 1305 Size: 2277 2025 184 2007 Fragments arranged by size: 2277 2025 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 2652 10 Size: 54 1425 343 663 157 3851 Fragments arranged by size: 3851 1425 663 343 157 54 MslI CAynn'nnrTG Cuts at: 518 1166 3155 3422 4451 4810 4969 518 Size: 648 1989 267 1029 359 159 2042 Fragments arranged by size: 2042 1989 1029 648 359 267 159 MspA1I CmG'CkG Cuts at: 1606 2264 2457 3013 3037 3909 4586 5734 5979 Size: 658 193 556 24 872 677 1148 245 Cuts at: 5979 1606 Size: 2120 Fragments arranged by size: 2120 1148 872 677 658 556 245 193 24 NcoI C'CATG_G Cuts at: 513 1065 2004 3153 513 Size: 552 939 1149 3853 Fragments arranged by size: 3853 1149 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 3400 3427 3851 5396 1132 Size: 97 2171 27 424 1545 2229 Fragments arranged by size: 2229 2171 1545 424 97 27 PasI CC'CwG_GG Cuts at: 1348 2382 1348 Size: 1034 5459 PciI A'CATG_T Cuts at: 3396 5392 3396 Size: 1996 4497 PflMI CCAn_nnn'nTGG Cuts at: 1108 2773 1108 Size: 1665 4828 PfoI T'CCnGG_A Cuts at: 2371 2371 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2072 3336 2072 Size: 1264 5229 PshAI GACnn'nnGTC Cuts at: 1613 2324 1613 Size: 711 5782 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 2258 2279 3582 2258 Size: 21 1303 5169 Fragments arranged by size: 5169 1303 21 PspXI vC'TCGA_Gb Cuts at: 2677 2677 Size: 6493 PstI C_TGCA'G Cuts at: 830 2101 3845 830 Size: 1271 1744 3478 Fragments arranged by size: 3478 1744 1271 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2457 3013 3037 2457 Size: 556 24 5913 Fragments arranged by size: 5913 556 24 SacI G_AGCT'C Cuts at: 2526 3105 2526 Size: 579 5914 SacII CC_GC'GG Cuts at: 2265 2265 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SanDI GG'GwC_CC Cuts at: 3336 3336 Size: 6493 SapI GCTCTTCn'nnn_ Cuts at: 3110 3110 Size: 6493 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SfcI C'TryA_G Cuts at: 826 1045 2097 3841 4976 5657 5848 826 Size: 219 1052 1744 1135 681 191 1471 Fragments arranged by size: 1744 1471 1135 1052 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2649 2649 Size: 6493 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2677 2930 3224 3665 3683 4597 Size: 197 926 734 253 294 441 18 914 Cuts at: 4597 5498 5760 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 914 901 734 441 294 262 253 197 18 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3851 1132 Size: 2719 3774 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 2928 1517 Size: 1411 5082 StyI C'CwwG_G Cuts at: 513 1065 2004 2224 2337 2577 2865 2943 3153 Size: 552 939 220 113 240 288 78 210 Cuts at: 3153 3932 513 Size: 779 3074 Fragments arranged by size: 3074 939 779 552 288 240 220 210 113 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2161 3155 4520 4537 4690 4875 5320 Size: 1255 581 994 1365 17 153 185 445 Cuts at: 5320 325 Size: 1498 Fragments arranged by size: 1498 1365 1255 994 581 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3084 4739 6451 Size: 274 80 33 51 1443 1107 1655 1712 Cuts at: 6451 96 Size: 138 Fragments arranged by size: 1712 1655 1443 1107 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6453 6485 6453 Size: 32 6461 Tth111I GACn'n_nGTC Cuts at: 1316 3551 1316 Size: 2235 4258 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3262 3262 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 2677 1943 Size: 734 5759 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 3719 4622 3719 Size: 903 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SexAI SfoI SrfI SwaI