Restriction map for
pFN21AE0985
(Linear) (Six-base) MAPSORT of: KIEE0985.seq Check: 2944 from: 1 to: 1788
KIEE0985.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 438 1788
Size: 438 1350
Acc65I G'GTAC_C
Cuts at: 0 190 1788
Size: 190 1598
AclI AA'CG_TT
Cuts at: 0 1478 1788
Size: 1478 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1336 1788
Size: 169 1167 452
Fragments arranged by size:
1167 452 169
AfeI AGC'GCT
Cuts at: 0 1731 1788
Size: 1731 57
AflII C'TTAA_G
Cuts at: 0 1660 1678 1788
Size: 1660 18 110
Fragments arranged by size:
1660 110 18
AflIII A'CryG_T
Cuts at: 0 1391 1788
Size: 1391 397
AleI CACnn'nnGTG
Cuts at: 0 1150 1788
Size: 1150 638
ApaI G_GGCC'C
Cuts at: 0 257 278 1581 1788
Size: 257 21 1303 207
Fragments arranged by size:
1303 257 207 21
ApoI r'AATT_y
Cuts at: 0 32 844 1788
Size: 32 812 944
Fragments arranged by size:
944 812 32
AvaI C'yCGr_G
Cuts at: 0 672 1761 1788
Size: 672 1089 27
Fragments arranged by size:
1089 672 27
BamHI G'GATC_C
Cuts at: 0 135 363 558 1178 1788
Size: 135 228 195 620 610
Fragments arranged by size:
620 610 228 195 135
BanI G'GyrC_C
Cuts at: 0 57 190 534 1788
Size: 57 133 344 1254
Fragments arranged by size:
1254 344 133 57
BanII G_rGCy'C
Cuts at: 0 257 278 427 521 1100 1581 1788
Size: 257 21 149 94 579 481 207
Fragments arranged by size:
579 481 257 207 149 94 21
BbvCI CC'TCA_GC
Cuts at: 0 820 1788
Size: 820 968
BclI T'GATC_A
Cuts at: 0 1409 1788
Size: 1409 379
BglI GCCn_nnn'nGGC
Cuts at: 0 644 1788
Size: 644 1144
BglII A'GATC_T
Cuts at: 0 1166 1670 1788
Size: 1166 504 118
Fragments arranged by size:
1166 504 118
BlpI GC'TnA_GC
Cuts at: 0 338 1426 1788
Size: 338 1088 362
Fragments arranged by size:
1088 362 338
Bme1580I G_kGCm'C
Cuts at: 0 257 278 539 1581 1770 1788
Size: 257 21 261 1042 189 18
Fragments arranged by size:
1042 261 257 189 21 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 1590 1788
Size: 1590 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1006 1788
Size: 453 553 782
Fragments arranged by size:
782 553 453
Bpu10I CC'TnA_GC
Cuts at: 0 820 1788
Size: 820 968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1204 1788
Size: 910 294 584
Fragments arranged by size:
910 584 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 1459 1504 1788
Size: 307 1152 45 284
Fragments arranged by size:
1152 307 284 45
BsaHI Gr'CG_yC
Cuts at: 0 1656 1788
Size: 1656 132
BsaWI w'CCGG_w
Cuts at: 0 132 1788
Size: 132 1656
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1360 1390 1534 1564 1788
Size: 1360 30 144 30 224
Fragments arranged by size:
1360 224 144 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 1788
Size: 763 1025
BseYI C'CCAG_C
Cuts at: 0 27 271 293 448 1788
Size: 27 244 22 155 1340
Fragments arranged by size:
1340 244 155 27 22
BsiHKAI G_wGCw'C
Cuts at: 0 521 1100 1788
Size: 521 579 688
Fragments arranged by size:
688 579 521
Bsp1286I G_dGCh'C
Cuts at: 0 257 278 427 521 539 1100 1581 1770
Size: 257 21 149 94 18 561 481 189
Cuts at: 1770 1788
Size: 18
Fragments arranged by size:
561 481 257 189 149 94 21 18
18
BspEI T'CCGG_A
Cuts at: 0 132 1788
Size: 132 1656
BspHI T'CATG_A
Cuts at: 0 998 1691 1788
Size: 998 693 97
Fragments arranged by size:
998 693 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 438 910 1788
Size: 438 472 878
Fragments arranged by size:
878 472 438
BsrBI CCG'CTC
Cuts at: 0 1430 1788
Size: 1430 358
BsrDI GCAATG_nn'
Cuts at: 0 1718 1788
Size: 1718 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 367 1788
Size: 367 1421
BstYI r'GATC_y
Cuts at: 0 135 363 558 1166 1178 1670 1788
Size: 135 228 195 608 12 492 118
Fragments arranged by size:
608 492 228 195 135 118 12
Bsu36I CC'TnA_GG
Cuts at: 0 351 1352 1788
Size: 351 1001 436
Fragments arranged by size:
1001 436 351
BtgI C'CryG_G
Cuts at: 0 257 554 1148 1788
Size: 257 297 594 640
Fragments arranged by size:
640 594 297 257
BtsI GCAGTG_nn'
Cuts at: 0 273 846 1788
Size: 273 573 942
Fragments arranged by size:
942 573 273
EaeI y'GGCC_r
Cuts at: 0 488 645 1788
Size: 488 157 1143
Fragments arranged by size:
1143 488 157
EarI CTCTTCn'nnn_
Cuts at: 0 1105 1189 1502 1690 1788
Size: 1105 84 313 188 98
Fragments arranged by size:
1105 313 188 98 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1106 1645 1788
Size: 1106 539 143
Fragments arranged by size:
1106 539 143
EcoICRI GAG'CTC
Cuts at: 0 519 1098 1788
Size: 519 579 690
Fragments arranged by size:
690 579 519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 453 1006 1336 1788
Size: 169 284 553 330 452
Fragments arranged by size:
553 452 330 284 169
EcoO109I rG'GnC_Cy
Cuts at: 0 67 223 253 275 726 1331 1577 1788
Size: 67 156 30 22 451 605 246 211
Fragments arranged by size:
605 451 246 211 156 67 30 22
EcoRI G'AATT_C
Cuts at: 0 32 844 1788
Size: 32 812 944
Fragments arranged by size:
944 812 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 1731 1788
Size: 1341 390 57
Fragments arranged by size:
1341 390 57
HaeII r_GCGC'y
Cuts at: 0 871 1704 1733 1788
Size: 871 833 29 55
Fragments arranged by size:
871 833 55 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1330 1360 1362 1392 1510 1534 1542 1566
Size: 1330 30 2 30 118 24 8 24
Cuts at: 1566 1788
Size: 222
Fragments arranged by size:
1330 222 118 30 30 24 24 8
2
HincII GTy'rAC
Cuts at: 0 1245 1788
Size: 1245 543
KpnI G_GTAC'C
Cuts at: 0 194 1788
Size: 194 1594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1577 1788
Size: 1577 211
MscI TGG'CCA
Cuts at: 0 490 647 1788
Size: 490 157 1141
Fragments arranged by size:
1141 490 157
MslI CAynn'nnrTG
Cuts at: 0 1150 1417 1788
Size: 1150 267 371
Fragments arranged by size:
1150 371 267
MspA1I CmG'CkG
Cuts at: 0 259 452 1008 1032 1788
Size: 259 193 556 24 756
Fragments arranged by size:
756 556 259 193 24
NcoI C'CATG_G
Cuts at: 0 1148 1788
Size: 1148 640
NspI r_CATG'y
Cuts at: 0 1395 1422 1788
Size: 1395 27 366
Fragments arranged by size:
1395 366 27
PasI CC'CwG_GG
Cuts at: 0 377 1788
Size: 377 1411
PciI A'CATG_T
Cuts at: 0 1391 1788
Size: 1391 397
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1788
Size: 768 1020
PfoI T'CCnGG_A
Cuts at: 0 366 1788
Size: 366 1422
PpuMI rG'GwC_Cy
Cuts at: 0 67 1331 1788
Size: 67 1264 457
Fragments arranged by size:
1264 457 67
PshAI GACnn'nnGTC
Cuts at: 0 319 1788
Size: 319 1469
PspOMI G'GGCC_C
Cuts at: 0 253 274 1577 1788
Size: 253 21 1303 211
Fragments arranged by size:
1303 253 211 21
PspXI vC'TCGA_Gb
Cuts at: 0 672 1788
Size: 672 1116
PstI C_TGCA'G
Cuts at: 0 96 1788
Size: 96 1692
PvuII CAG'CTG
Cuts at: 0 452 1008 1032 1788
Size: 452 556 24 756
Fragments arranged by size:
756 556 452 24
SacI G_AGCT'C
Cuts at: 0 521 1100 1788
Size: 521 579 688
Fragments arranged by size:
688 579 521
SacII CC_GC'GG
Cuts at: 0 260 1788
Size: 260 1528
SanDI GG'GwC_CC
Cuts at: 0 1331 1788
Size: 1331 457
SapI GCTCTTCn'nnn_
Cuts at: 0 1105 1788
Size: 1105 683
SfcI C'TryA_G
Cuts at: 0 92 1788
Size: 92 1696
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 644 1788
Size: 644 1144
SmlI C'TyrA_G
Cuts at: 0 672 925 1219 1660 1678 1788
Size: 672 253 294 441 18 110
Fragments arranged by size:
672 441 294 253 110 18
StuI AGG'CCT
Cuts at: 0 923 1788
Size: 923 865
StyI C'CwwG_G
Cuts at: 0 219 332 572 860 938 1148 1788
Size: 219 113 240 288 78 210 640
Fragments arranged by size:
640 288 240 219 210 113 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 1150 1788
Size: 156 994 638
Fragments arranged by size:
994 638 156
TatI w'GTAC_w
Cuts at: 0 1079 1788
Size: 1079 709
Tth111I GACn'n_nGTC
Cuts at: 0 1546 1788
Size: 1546 242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1257 1788
Size: 1257 531
XhoI C'TCGA_G
Cuts at: 0 672 1788
Size: 672 1116
XmnI GAAnn'nnTTC
Cuts at: 0 1714 1788
Size: 1714 74
Enzymes that do cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AleI
ApaI ApoI AvaI BamHI BanI BanII BbvCI BclI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII KpnI MmeI MscI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SanDI SapI SfcI SfiI SmlI StuI StyI TaqII
TatI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AgeI AhdI AlfI AloI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI
BciVI BfrBI BmgBI BmtI BplI BsaAI BsaBI BsgI
BsiEI BsiWI BsmI BsmBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PsiI PsrI PvuI RsrII SalI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XmaI ZraI