(Circular) (Six-base) MAPSORT of: pFN21AE0967.seq Check: 8743 from: 1 to: 6244 pFN21AE0967 6244 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4054 278 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2709 3587 1937 Size: 772 878 4594 Fragments arranged by size: 4594 878 772 Acc65I G'GTAC_C Cuts at: 3086 3565 3086 Size: 479 5765 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 2319 4307 5691 5959 1632 Size: 408 279 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 408 279 268 AflII C'TTAA_G Cuts at: 820 1017 2840 820 Size: 197 1823 4224 Fragments arranged by size: 4224 1823 197 AflIII A'CryG_T Cuts at: 1203 3237 5143 6120 1203 Size: 2034 1906 977 1327 Fragments arranged by size: 2034 1906 1327 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 2683 4973 2683 Size: 2290 3954 AleI CACnn'nnGTG Cuts at: 1166 3503 1166 Size: 2337 3907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2202 3363 1184 Size: 1018 1161 4065 Fragments arranged by size: 4065 1161 1018 AlwNI CAG_nnn'CTG Cuts at: 3987 5559 3987 Size: 1572 4672 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 3041 3553 3779 3931 6040 1493 Size: 1548 512 226 152 2109 1697 Fragments arranged by size: 2109 1697 1548 512 226 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 3569 1870 Size: 73 1626 4545 Fragments arranged by size: 4545 1626 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2437 2771 3086 3565 5020 Size: 325 217 627 650 334 315 479 1455 Cuts at: 5020 618 Size: 1842 Fragments arranged by size: 1842 1455 650 627 479 334 325 315 217 BanII G_rGCy'C Cuts at: 1391 3563 1391 Size: 2172 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3288 928 Size: 971 1389 3884 Fragments arranged by size: 3884 1389 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 2799 1548 Size: 1251 4993 BfrBI ATG'CAT Cuts at: 3146 3146 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4855 136 Size: 107 122 71 631 605 3183 1525 Fragments arranged by size: 3183 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 3661 3661 Size: 6244 Bme1580I G_kGCm'C Cuts at: 1790 4305 5461 1790 Size: 2515 1156 2573 Fragments arranged by size: 2573 2515 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6244 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2626 3975 4933 Size: 416 53 83 399 24 1209 1349 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1753 1349 1209 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3090 4904 1239 Size: 1851 1814 2579 Fragments arranged by size: 2579 1851 1814 Bpu10I CC'TnA_GC Cuts at: 2400 2400 Size: 6244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2514 4369 5234 5532 2514 Size: 1855 865 298 3226 Fragments arranged by size: 3226 1855 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2860 4907 882 Size: 617 1361 2047 2219 Fragments arranged by size: 2219 2047 1361 617 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 2787 6238 2787 Size: 3451 2793 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4051 4433 5068 275 Size: 53 83 186 936 2518 382 635 1451 Fragments arranged by size: 2518 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2356 4677 5075 5349 5496 5907 2356 Size: 2321 398 274 147 411 2693 Fragments arranged by size: 2693 2321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6019 Fragments arranged by size: 6019 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3266 1186 Size: 2080 4164 BseYI C'CCAG_C Cuts at: 5447 5943 5447 Size: 496 5748 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2778 1163 Size: 573 1042 4629 Fragments arranged by size: 4629 1042 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3694 4455 5483 1540 Size: 275 186 1693 761 1028 2301 Fragments arranged by size: 2301 1693 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4305 4390 5461 4305 Size: 85 1071 5088 Fragments arranged by size: 5088 1071 85 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3563 4305 4390 5461 1391 Size: 399 1773 742 85 1071 2174 Fragments arranged by size: 2174 1773 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2356 5907 2356 Size: 3551 2693 BspHI T'CATG_A Cuts at: 2812 4133 5863 2812 Size: 1321 1730 3193 Fragments arranged by size: 3193 1730 1321 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2959 3599 844 Size: 2115 640 3489 Fragments arranged by size: 3489 2115 640 BsrBI CCG'CTC Cuts at: 1394 1823 4131 1394 Size: 429 2308 3507 Fragments arranged by size: 3507 2308 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3015 3366 4739 4913 65 Size: 1183 1767 351 1373 174 1396 Fragments arranged by size: 1767 1396 1373 1183 351 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4888 5075 1700 Size: 110 3078 187 2869 Fragments arranged by size: 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2484 96 Size: 2388 3856 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6244 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstYI r'GATC_y Cuts at: 1972 2788 3574 3941 4326 4343 5795 5881 5893 Size: 816 786 367 385 17 1452 86 12 Cuts at: 5893 6239 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 816 786 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2889 3073 3171 513 Size: 552 939 885 184 98 3586 Fragments arranged by size: 3586 939 885 552 184 98 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 2576 3760 4554 4574 2576 Size: 1184 794 20 4246 Fragments arranged by size: 4246 1184 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 3548 3548 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5251 809 Size: 507 3935 1802 Fragments arranged by size: 3935 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2941 3262 3691 Size: 54 1425 50 275 18 1111 321 429 Cuts at: 3691 4580 6209 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1111 889 429 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4174 1353 Size: 2821 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4831 5205 5351 387 Size: 1064 138 3242 374 146 1280 Fragments arranged by size: 3242 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2319 3090 4307 4904 5691 5959 Size: 393 408 279 771 1217 597 787 268 Cuts at: 5959 1239 Size: 1524 Fragments arranged by size: 1524 1217 787 771 597 408 393 279 268 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 EcoRV GAT'ATC Cuts at: 2251 2251 Size: 6244 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 5391 1678 Size: 3713 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 4885 4917 4959 Size: 32 135 32 1097 32 2184 32 42 Cuts at: 4959 4991 1373 Size: 32 2626 Fragments arranged by size: 2626 2184 1097 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3517 3588 3834 669 Size: 988 281 1579 71 246 3079 Fragments arranged by size: 3079 1579 988 281 246 71 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KpnI G_GTAC'C Cuts at: 3090 3569 3090 Size: 479 5765 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2549 5358 5542 1305 Size: 1244 2809 184 2007 Fragments arranged by size: 2809 2007 1244 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2943 3264 10 Size: 54 1425 343 1111 321 2990 Fragments arranged by size: 2990 1425 1111 343 321 54 MslI CAynn'nnrTG Cuts at: 518 1166 3503 4202 4561 4720 518 Size: 648 2337 699 359 159 2042 Fragments arranged by size: 2337 2042 699 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3419 3660 4337 5485 5730 1606 Size: 1813 241 677 1148 245 2120 Fragments arranged by size: 2120 1813 1148 677 245 241 NcoI C'CATG_G Cuts at: 513 1065 2004 2889 3073 3171 513 Size: 552 939 885 184 98 3586 Fragments arranged by size: 3586 939 885 552 184 98 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NsiI A_TGCA'T Cuts at: 3148 3148 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 3241 3289 3350 3602 5147 1132 Size: 97 2012 48 61 252 1545 2229 Fragments arranged by size: 2229 2012 1545 252 97 61 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6244 PciI A'CATG_T Cuts at: 3237 5143 3237 Size: 1906 4338 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6244 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PstI C_TGCA'G Cuts at: 830 2583 3064 3217 3596 830 Size: 1753 481 153 379 3478 Fragments arranged by size: 3478 1753 481 379 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 3419 3419 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SfcI C'TryA_G Cuts at: 826 1045 2579 2710 3060 3213 3225 3592 4727 Size: 219 1534 131 350 153 12 367 1135 Cuts at: 4727 5408 5599 826 Size: 681 191 1471 Fragments arranged by size: 1534 1471 1135 681 367 350 219 191 153 131 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2529 2840 4348 5249 5511 820 Size: 197 926 586 311 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 586 311 262 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 2203 2664 3466 4168 5 Size: 47 2151 461 802 702 2081 Fragments arranged by size: 2151 2081 802 702 461 47 StuI AGG'CCT Cuts at: 1517 3319 1517 Size: 1802 4442 StyI C'CwwG_G Cuts at: 513 1065 2004 2433 2889 3073 3171 3683 513 Size: 552 939 429 456 184 98 512 3074 Fragments arranged by size: 3074 939 552 512 456 429 184 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2470 4271 4288 4441 4626 5071 325 Size: 1255 890 1801 17 153 185 445 1498 Fragments arranged by size: 1801 1498 1255 890 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 2515 3206 Size: 274 80 33 51 1443 507 31 691 Cuts at: 3206 4490 6202 96 Size: 1284 1712 138 Fragments arranged by size: 1712 1443 1284 691 507 274 138 80 51 33 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2260 2292 2371 2403 6204 6236 2260 Size: 32 79 32 3801 32 2268 Fragments arranged by size: 3801 2268 79 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2782 3080 2782 Size: 298 5946 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4052 276 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI