Restriction map for
pFN21AE0967
(Linear) (Six-base) MAPSORT of: KIEE0967.seq Check: 8796 from: 1 to: 1539
KIEE0967.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 704 1539
Size: 704 835
Acc65I G'GTAC_C
Cuts at: 0 1081 1539
Size: 1081 458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 314 1539
Size: 35 279 1225
Fragments arranged by size:
1225 279 35
AflII C'TTAA_G
Cuts at: 0 835 1539
Size: 835 704
AflIII A'CryG_T
Cuts at: 0 1232 1539
Size: 1232 307
AhdI GACnn_n'nnGTC
Cuts at: 0 678 1539
Size: 678 861
AleI CACnn'nnGTG
Cuts at: 0 1498 1539
Size: 1498 41
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 197 1358 1539
Size: 197 1161 181
Fragments arranged by size:
1161 197 181
ApoI r'AATT_y
Cuts at: 0 1036 1539
Size: 1036 503
BanI G'GyrC_C
Cuts at: 0 432 766 1081 1539
Size: 432 334 315 458
Fragments arranged by size:
458 432 334 315
BbsI GAAGACnn'nnnn_
Cuts at: 0 1283 1539
Size: 1283 256
BclI T'GATC_A
Cuts at: 0 794 1539
Size: 794 745
BfrBI ATG'CAT
Cuts at: 0 1141 1539
Size: 1141 398
BmrI ACTGGGnnnn_n'
Cuts at: 0 621 1539
Size: 621 918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1539
Size: 1085 454
Bpu10I CC'TnA_GC
Cuts at: 0 395 1539
Size: 395 1144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 1539
Size: 509 1030
BsaI GGTCTCn'nnnn_
Cuts at: 0 855 1539
Size: 855 684
BsaBI GATnn'nnATC
Cuts at: 0 782 1539
Size: 782 757
BsaWI w'CCGG_w
Cuts at: 0 351 1539
Size: 351 1188
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1261 1539
Size: 1261 278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1539
Size: 773 766
BspEI T'CCGG_A
Cuts at: 0 351 1539
Size: 351 1188
BspHI T'CATG_A
Cuts at: 0 807 1539
Size: 807 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 954 1539
Size: 954 585
BsrDI GCAATG_nn'
Cuts at: 0 1010 1361 1539
Size: 1010 351 178
Fragments arranged by size:
1010 351 178
BsrGI T'GTAC_A
Cuts at: 0 479 1539
Size: 479 1060
BstYI r'GATC_y
Cuts at: 0 783 1539
Size: 783 756
BtgI C'CryG_G
Cuts at: 0 884 1068 1166 1539
Size: 884 184 98 373
Fragments arranged by size:
884 373 184 98
BtsI GCAGTG_nn'
Cuts at: 0 571 1539
Size: 571 968
EaeI y'GGCC_r
Cuts at: 0 936 1257 1539
Size: 936 321 282
Fragments arranged by size:
936 321 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 314 1085 1539
Size: 35 279 771 454
Fragments arranged by size:
771 454 279 35
EcoRV GAT'ATC
Cuts at: 0 246 1539
Size: 246 1293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 1539
Size: 664 32 843
Fragments arranged by size:
843 664 32
HincII GTy'rAC
Cuts at: 0 1512 1539
Size: 1512 27
KpnI G_GTAC'C
Cuts at: 0 1085 1539
Size: 1085 454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1539
Size: 544 995
MscI TGG'CCA
Cuts at: 0 938 1259 1539
Size: 938 321 280
Fragments arranged by size:
938 321 280
MslI CAynn'nnrTG
Cuts at: 0 1498 1539
Size: 1498 41
MspA1I CmG'CkG
Cuts at: 0 1414 1539
Size: 1414 125
NcoI C'CATG_G
Cuts at: 0 884 1068 1166 1539
Size: 884 184 98 373
Fragments arranged by size:
884 373 184 98
NsiI A_TGCA'T
Cuts at: 0 1143 1539
Size: 1143 396
NspI r_CATG'y
Cuts at: 0 1236 1284 1345 1539
Size: 1236 48 61 194
Fragments arranged by size:
1236 194 61 48
PciI A'CATG_T
Cuts at: 0 1232 1539
Size: 1232 307
PstI C_TGCA'G
Cuts at: 0 578 1059 1212 1539
Size: 578 481 153 327
Fragments arranged by size:
578 481 327 153
PvuII CAG'CTG
Cuts at: 0 1414 1539
Size: 1414 125
SfcI C'TryA_G
Cuts at: 0 574 705 1055 1208 1220 1539
Size: 574 131 350 153 12 319
Fragments arranged by size:
574 350 319 153 131 12
SmlI C'TyrA_G
Cuts at: 0 524 835 1539
Size: 524 311 704
Fragments arranged by size:
704 524 311
SspI AAT'ATT
Cuts at: 0 198 659 1461 1539
Size: 198 461 802 78
Fragments arranged by size:
802 461 198 78
StuI AGG'CCT
Cuts at: 0 1314 1539
Size: 1314 225
StyI C'CwwG_G
Cuts at: 0 428 884 1068 1166 1539
Size: 428 456 184 98 373
Fragments arranged by size:
456 428 373 184 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 1539
Size: 465 1074
TatI w'GTAC_w
Cuts at: 0 479 510 1201 1539
Size: 479 31 691 338
Fragments arranged by size:
691 479 338 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 255 287 366 398 1539
Size: 255 32 79 32 1141
Fragments arranged by size:
1141 255 79 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 777 1075 1539
Size: 777 298 464
Fragments arranged by size:
777 464 298
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AleI AlfI
ApoI BanI BbsI BclI BfrBI BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BseRI BsgI BspEI BspHI
BspMI BsrDI BsrGI BstYI BtgI BtsI EaeI Eco57MI
EcoRV Hin4I HincII KpnI MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PstI PvuII SfcI SmlI
SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BsaAI BsaHI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI