Restriction map for
pFN21AE0959
(Linear) (Six-base) MAPSORT of: KIEE0959.seq Check: 5922 from: 1 to: 1497
KIEE0959.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1364 1497
Size: 1364 133
AlwNI CAG_nnn'CTG
Cuts at: 0 109 684 787 942 1155 1218 1289 1497
Size: 109 575 103 155 213 63 71 208
Fragments arranged by size:
575 213 208 155 109 103 71 63
ApaI G_GGCC'C
Cuts at: 0 442 456 575 728 1143 1225 1497
Size: 442 14 119 153 415 82 272
Fragments arranged by size:
442 415 272 153 119 82 14
ApaLI G'TGCA_C
Cuts at: 0 688 1377 1497
Size: 688 689 120
Fragments arranged by size:
689 688 120
AvaI C'yCGr_G
Cuts at: 0 728 826 1427 1497
Size: 728 98 601 70
Fragments arranged by size:
728 601 98 70
AvrII C'CTAG_G
Cuts at: 0 1305 1497
Size: 1305 192
BanI G'GyrC_C
Cuts at: 0 46 67 221 604 1497
Size: 46 21 154 383 893
Fragments arranged by size:
893 383 154 46 21
BanII G_rGCy'C
Cuts at: 0 140 283 442 456 575 728 844 1143
Size: 140 143 159 14 119 153 116 299
Cuts at: 1143 1225 1435 1497
Size: 82 210 62
Fragments arranged by size:
299 210 159 153 143 140 119 116
82 62 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 363 977 1497
Size: 363 614 520
Fragments arranged by size:
614 520 363
BbvCI CC'TCA_GC
Cuts at: 0 1465 1497
Size: 1465 32
BglI GCCn_nnn'nGGC
Cuts at: 0 135 347 1060 1497
Size: 135 212 713 437
Fragments arranged by size:
713 437 212 135
BlpI GC'TnA_GC
Cuts at: 0 139 337 1497
Size: 139 198 1160
Fragments arranged by size:
1160 198 139
Bme1580I G_kGCm'C
Cuts at: 0 49 442 456 575 692 728 1143 1225
Size: 49 393 14 119 117 36 415 82
Cuts at: 1225 1381 1497
Size: 156 116
Fragments arranged by size:
415 393 156 119 117 116 82 49
36 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 1299 1497
Size: 73 1226 198
Fragments arranged by size:
1226 198 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1039 1266 1497
Size: 538 501 227 231
Fragments arranged by size:
538 501 231 227
Bpu10I CC'TnA_GC
Cuts at: 0 1126 1465 1497
Size: 1126 339 32
Fragments arranged by size:
1126 339 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 1497
Size: 106 1391
BsaI GGTCTCn'nnnn_
Cuts at: 0 800 1497
Size: 800 697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 547 1415 1453 1497
Size: 194 353 868 38 44
Fragments arranged by size:
868 353 194 44 38
BseYI C'CCAG_C
Cuts at: 0 84 574 744 771 1497
Size: 84 490 170 27 726
Fragments arranged by size:
726 490 170 84 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 655 1497
Size: 2 653 842
Fragments arranged by size:
842 653 2
BsiEI CG_ry'CG
Cuts at: 0 399 1049 1497
Size: 399 650 448
Fragments arranged by size:
650 448 399
BsiHKAI G_wGCw'C
Cuts at: 0 692 1357 1381 1497
Size: 692 665 24 116
Fragments arranged by size:
692 665 116 24
BsmI GAATG_Cn'
Cuts at: 0 179 1497
Size: 179 1318
Bsp1286I G_dGCh'C
Cuts at: 0 49 140 283 442 456 575 692 728
Size: 49 91 143 159 14 119 117 36
Cuts at: 728 844 1143 1225 1357 1381 1435 1497
Size: 116 299 82 132 24 54 62
Fragments arranged by size:
299 159 143 132 119 117 116 91
82 62 54 49 36 24 14
BspHI T'CATG_A
Cuts at: 0 1010 1497
Size: 1010 487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 560 647 965 1364 1497
Size: 560 87 318 399 133
Fragments arranged by size:
560 399 318 133 87
BsrFI r'CCGG_y
Cuts at: 0 299 398 976 1497
Size: 299 99 578 521
Fragments arranged by size:
578 521 299 99
BssSI C'ACGA_G
Cuts at: 0 481 1497
Size: 481 1016
BstAPI GCAn_nnn'nTGC
Cuts at: 0 228 942 1155 1497
Size: 228 714 213 342
Fragments arranged by size:
714 342 228 213
Bsu36I CC'TnA_GG
Cuts at: 0 617 1325 1497
Size: 617 708 172
Fragments arranged by size:
708 617 172
BtgI C'CryG_G
Cuts at: 0 38 351 918 1043 1084 1497
Size: 38 313 567 125 41 413
Fragments arranged by size:
567 413 313 125 41 38
BtsI GCAGTG_nn'
Cuts at: 0 971 1063 1497
Size: 971 92 434
Fragments arranged by size:
971 434 92
EaeI y'GGCC_r
Cuts at: 0 23 301 396 464 1046 1497
Size: 23 278 95 68 582 451
Fragments arranged by size:
582 451 278 95 68 23
EagI C'GGCC_G
Cuts at: 0 396 1046 1497
Size: 396 650 451
Fragments arranged by size:
650 451 396
EarI CTCTTCn'nnn_
Cuts at: 0 202 1497
Size: 202 1295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 149 1497
Size: 85 64 1348
Fragments arranged by size:
1348 85 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1039 1266 1497
Size: 538 501 227 231
Fragments arranged by size:
538 501 231 227
EcoNI CCTnn'n_nnAGG
Cuts at: 0 616 1147 1474 1497
Size: 616 531 327 23
Fragments arranged by size:
616 531 327 23
EcoO109I rG'GnC_Cy
Cuts at: 0 438 439 681 724 725 1053 1122 1140
Size: 438 1 242 43 1 328 69 18
Cuts at: 1140 1152 1309 1497
Size: 12 157 188
Fragments arranged by size:
438 328 242 188 157 69 43 18
12 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 1399 1431 1497
Size: 104 32 1263 32 66
Fragments arranged by size:
1263 104 66 32 32
MscI TGG'CCA
Cuts at: 0 25 1497
Size: 25 1472
MslI CAynn'nnrTG
Cuts at: 0 31 695 1497
Size: 31 664 802
Fragments arranged by size:
802 664 31
MspA1I CmG'CkG
Cuts at: 0 228 737 859 942 1086 1117 1205 1497
Size: 228 509 122 83 144 31 88 292
Fragments arranged by size:
509 292 228 144 122 88 83 31
NaeI GCC'GGC
Cuts at: 0 301 400 1497
Size: 301 99 1097
Fragments arranged by size:
1097 301 99
NcoI C'CATG_G
Cuts at: 0 351 1497
Size: 351 1146
NgoMIV G'CCGG_C
Cuts at: 0 299 398 1497
Size: 299 99 1099
Fragments arranged by size:
1099 299 99
NspI r_CATG'y
Cuts at: 0 902 1497
Size: 902 595
PasI CC'CwG_GG
Cuts at: 0 131 283 1218 1385 1497
Size: 131 152 935 167 112
Fragments arranged by size:
935 167 152 131 112
PflMI CCAn_nnn'nTGG
Cuts at: 0 684 1289 1497
Size: 684 605 208
Fragments arranged by size:
684 605 208
PfoI T'CCnGG_A
Cuts at: 0 797 887 1265 1497
Size: 797 90 378 232
Fragments arranged by size:
797 378 232 90
PspOMI G'GGCC_C
Cuts at: 0 438 452 571 724 1139 1221 1497
Size: 438 14 119 153 415 82 276
Fragments arranged by size:
438 415 276 153 119 82 14
PstI C_TGCA'G
Cuts at: 0 233 278 557 584 839 1258 1497
Size: 233 45 279 27 255 419 239
Fragments arranged by size:
419 279 255 239 233 45 27
PvuII CAG'CTG
Cuts at: 0 228 942 1205 1497
Size: 228 714 263 292
Fragments arranged by size:
714 292 263 228
SacII CC_GC'GG
Cuts at: 0 1087 1497
Size: 1087 410
SfcI C'TryA_G
Cuts at: 0 229 274 553 580 835 1210 1254 1497
Size: 229 45 279 27 255 375 44 243
Fragments arranged by size:
375 279 255 243 229 45 44 27
SmaI CCC'GGG
Cuts at: 0 730 1497
Size: 730 767
SmlI C'TyrA_G
Cuts at: 0 121 1497
Size: 121 1376
SphI G_CATG'C
Cuts at: 0 902 1497
Size: 902 595
StuI AGG'CCT
Cuts at: 0 262 526 598 1497
Size: 262 264 72 899
Fragments arranged by size:
899 264 262 72
StyI C'CwwG_G
Cuts at: 0 170 351 386 443 1305 1497
Size: 170 181 35 57 862 192
Fragments arranged by size:
862 192 181 170 57 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 515 736 1398 1497
Size: 515 221 662 99
Fragments arranged by size:
662 515 221 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 749 781 1497
Size: 749 32 716
Fragments arranged by size:
749 716 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 717 891 1392 1497
Size: 106 611 174 501 105
Fragments arranged by size:
611 501 174 106 105
XmaI C'CCGG_G
Cuts at: 0 728 1497
Size: 728 769
Enzymes that do cut:
AarI AlwNI ApaI ApaLI AvaI AvrII BanI BanII
BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BssSI BstAPI Bsu36I BtgI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PspOMI PstI PvuII SacII SfcI
SmaI SmlI SphI StuI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI