Restriction map for pFN21AE0954
 (Linear) (Six-base) MAPSORT of: KIEE0954.seq  Check: 1784  from: 1  to: 1446



KIEE0954.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AclI AA'CG_TT

Cuts at:      0     167    1446 
   Size:        167    1279
 
AleI CACnn'nnGTG

Cuts at:      0    1165    1446 
   Size:       1165     281
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1040    1446 
   Size:       1040     406
 
ApaI G_GGCC'C

Cuts at:      0     191     771    1446 
   Size:        191     580     675

  Fragments arranged by size: 

                 675     580     191
 
AvaI C'yCGr_G

Cuts at:      0     589     887    1253    1446 
   Size:        589     298     366     193

  Fragments arranged by size: 

                 589     366     298     193
 
BanI G'GyrC_C

Cuts at:      0     265     667     691    1170    1235    1446 
   Size:        265     402      24     479      65     211

  Fragments arranged by size: 

                 479     402     265     211      65      24
 
BanII G_rGCy'C

Cuts at:      0      68     191     467     771    1325    1446 
   Size:         68     123     276     304     554     121

  Fragments arranged by size: 

                 554     304     276     123     121      68
 
BbeI G_GCGC'C

Cuts at:      0     671    1174    1239    1446 
   Size:        671     503      65     207

  Fragments arranged by size: 

                 671     503     207      65
 
BbvCI CC'TCA_GC

Cuts at:      0    1126    1446 
   Size:       1126     320
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1144    1446 
   Size:       1144     302
 
BlpI GC'TnA_GC

Cuts at:      0      67     353     604    1446 
   Size:         67     286     251     842

  Fragments arranged by size: 

                 842     286     251      67
 
Bme1580I G_kGCm'C

Cuts at:      0     191     771    1446 
   Size:        191     580     675

  Fragments arranged by size: 

                 675     580     191
 
BmgBI CAC'GTC

Cuts at:      0    1062    1446 
   Size:       1062     384
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1221    1446 
   Size:       1221     225
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1446 
   Size:        499     947
 
Bpu10I CC'TnA_GC

Cuts at:      0    1126    1446 
   Size:       1126     320
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     568    1258    1446 
   Size:        463     105     690     188

  Fragments arranged by size: 

                 690     463     188     105
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     148    1446 
   Size:        148    1298
 
BsaAI yAC'GTr

Cuts at:      0    1167    1446 
   Size:       1167     279
 
BsaHI Gr'CG_yC

Cuts at:      0     668    1096    1171    1236    1434    1446 
   Size:        668     428      75      65     198      12

  Fragments arranged by size: 

                 668     428     198      75      65      12
 
BsaWI w'CCGG_w

Cuts at:      0     818    1446 
   Size:        818     628
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1331    1361    1446 
   Size:       1331      30      85

  Fragments arranged by size: 

                1331      85      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1069    1446 
   Size:       1069     377
 
BseYI C'CCAG_C

Cuts at:      0     216     922    1303    1309    1446 
   Size:        216     706     381       6     137

  Fragments arranged by size: 

                 706     381     216     137       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1446 
   Size:        985     461
 
BsiEI CG_ry'CG

Cuts at:      0       8    1446 
   Size:          8    1438
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     467    1446 
   Size:         68     399     979

  Fragments arranged by size: 

                 979     399      68
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      22    1446 
   Size:         22    1424
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     191     467     771    1325    1446 
   Size:         68     123     276     304     554     121

  Fragments arranged by size: 

                 554     304     276     123     121      68
 
BsrBI CCG'CTC

Cuts at:      0     357    1446 
   Size:        357    1089
 
BsrDI GCAATG_nn'

Cuts at:      0     330    1446 
   Size:        330    1116
 
BsrFI r'CCGG_y

Cuts at:      0    1098    1446 
   Size:       1098     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     421     934    1446 
   Size:        421     513     512

  Fragments arranged by size: 

                 513     512     421
 
Bsu36I CC'TnA_GG

Cuts at:      0     301    1401    1446 
   Size:        301    1100      45

  Fragments arranged by size: 

                1100     301      45
 
BtgI C'CryG_G

Cuts at:      0      74     830    1325    1428    1446 
   Size:         74     756     495     103      18

  Fragments arranged by size: 

                 756     495     103      74      18
 
BtsI GCAGTG_nn'

Cuts at:      0     192     735     859    1446 
   Size:        192     543     124     587

  Fragments arranged by size: 

                 587     543     192     124
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1413    1446 
   Size:       1413      33
 
EaeI y'GGCC_r

Cuts at:      0       5     732    1446 
   Size:          5     727     714

  Fragments arranged by size: 

                 727     714       5
 
EagI C'GGCC_G

Cuts at:      0       5    1446 
   Size:          5    1441
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     472    1446 
   Size:        131     341     974

  Fragments arranged by size: 

                 974     341     131
 
EcoICRI GAG'CTC

Cuts at:      0      66     465    1446 
   Size:         66     399     981

  Fragments arranged by size: 

                 981     399      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1446 
   Size:        499     947
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     304    1446 
   Size:        304    1142
 
EcoO109I rG'GnC_Cy

Cuts at:      0     188     579     767     768     891    1037    1257    1446 
   Size:        188     391     188       1     123     146     220     189

  Fragments arranged by size: 

                 391     220     189     188     188     146     123       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     402    1218    1446 
   Size:        402     816     228

  Fragments arranged by size: 

                 816     402     228
 
FspI TGC'GCA

Cuts at:      0     322     727    1446 
   Size:        322     405     719

  Fragments arranged by size: 

                 719     405     322
 
HaeII r_GCGC'y

Cuts at:      0     671    1174    1239    1446 
   Size:        671     503      65     207

  Fragments arranged by size: 

                 671     503     207      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     476     508    1446 
   Size:        476      32     938

  Fragments arranged by size: 

                 938     476      32
 
KasI G'GCGC_C

Cuts at:      0     667    1170    1235    1446 
   Size:        667     503      65     211

  Fragments arranged by size: 

                 667     503     211      65
 
MscI TGG'CCA

Cuts at:      0     734    1446 
   Size:        734     712
 
MslI CAynn'nnrTG

Cuts at:      0    1165    1446 
   Size:       1165     281
 
MspA1I CmG'CkG

Cuts at:      0      76     109     930    1446 
   Size:         76      33     821     516

  Fragments arranged by size: 

                 821     516      76      33
 
NarI GG'CG_CC

Cuts at:      0     668    1171    1236    1446 
   Size:        668     503      65     210

  Fragments arranged by size: 

                 668     503     210      65
 
NcoI C'CATG_G

Cuts at:      0     830    1446 
   Size:        830     616
 
NspI r_CATG'y

Cuts at:      0      86     786    1446 
   Size:         86     700     660

  Fragments arranged by size: 

                 700     660      86
 
PasI CC'CwG_GG

Cuts at:      0     543     561     946    1446 
   Size:        543      18     385     500

  Fragments arranged by size: 

                 543     500     385      18
 
PfoI T'CCnGG_A

Cuts at:      0     497    1181    1446 
   Size:        497     684     265

  Fragments arranged by size: 

                 684     497     265
 
PpuMI rG'GwC_Cy

Cuts at:      0     579     891    1257    1446 
   Size:        579     312     366     189

  Fragments arranged by size: 

                 579     366     312     189
 
PshAI GACnn'nnGTC

Cuts at:      0      83    1446 
   Size:         83    1363
 
PspOMI G'GGCC_C

Cuts at:      0     187     767    1446 
   Size:        187     580     679

  Fragments arranged by size: 

                 679     580     187
 
PstI C_TGCA'G

Cuts at:      0    1049    1446 
   Size:       1049     397
 
PvuII CAG'CTG

Cuts at:      0     109    1446 
   Size:        109    1337
 
SacI G_AGCT'C

Cuts at:      0      68     467    1446 
   Size:         68     399     979

  Fragments arranged by size: 

                 979     399      68
 
SacII CC_GC'GG

Cuts at:      0      77    1446 
   Size:         77    1369
 
SanDI GG'GwC_CC

Cuts at:      0    1257    1446 
   Size:       1257     189
 
SapI GCTCTTCn'nnn_

Cuts at:      0     472    1446 
   Size:        472     974
 
SfcI C'TryA_G

Cuts at:      0    1045    1446 
   Size:       1045     401
 
SfoI GGC'GCC

Cuts at:      0     669    1172    1237    1446 
   Size:        669     503      65     209

  Fragments arranged by size: 

                 669     503     209      65
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1098    1446 
   Size:       1098     348
 
SmaI CCC'GGG

Cuts at:      0     889    1255    1446 
   Size:        889     366     191

  Fragments arranged by size: 

                 889     366     191
 
SmlI C'TyrA_G

Cuts at:      0     442     583    1273    1446 
   Size:        442     141     690     173

  Fragments arranged by size: 

                 690     442     173     141
 
StuI AGG'CCT

Cuts at:      0    1147    1446 
   Size:       1147     299
 
StyI C'CwwG_G

Cuts at:      0     329     830    1446 
   Size:        329     501     616

  Fragments arranged by size: 

                 616     501     329
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     646    1245    1446 
   Size:        646     599     201

  Fragments arranged by size: 

                 646     599     201
 
TatI w'GTAC_w

Cuts at:      0     701    1446 
   Size:        701     745
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     924     956    1446 
   Size:        924      32     490

  Fragments arranged by size: 

                 924     490      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     895    1446 
   Size:        895     551
 
XbaI T'CTAG_A

Cuts at:      0     366    1446 
   Size:        366    1080
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     702    1446 
   Size:        702     744
 
XmaI C'CCGG_G

Cuts at:      0     887    1253    1446 
   Size:        887     366     193

  Fragments arranged by size: 

                 887     366     193
 

 Enzymes that do cut:

     AclI     AleI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
    BbvCI     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI
    BstXI   Bsu36I     BtgI     BtsI     DrdI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
     KasI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PasI
     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII
    SanDI     SapI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII     BmrI     BmtI    BsaBI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EciI
    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SbfI     ScaI
    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XhoI     XmnI     ZraI
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