Restriction map for pFN21AE0951
 (Linear) (Six-base) MAPSORT of: KIEE0951.seq  Check: 9522  from: 1  to: 1419



KIEE0951.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1250    1342    1419 
   Size:       1250      92      77

  Fragments arranged by size: 

                1250      92      77
 
AflII C'TTAA_G

Cuts at:      0     976    1419 
   Size:        976     443
 
AflIII A'CryG_T

Cuts at:      0     668    1419 
   Size:        668     751
 
AhdI GACnn_n'nnGTC

Cuts at:      0     672    1419 
   Size:        672     747
 
ApaI G_GGCC'C

Cuts at:      0    1149    1419 
   Size:       1149     270
 
ApoI r'AATT_y

Cuts at:      0     632     758     955    1130    1419 
   Size:        632     126     197     175     289

  Fragments arranged by size: 

                 632     289     197     175     126
 
AseI AT'TA_AT

Cuts at:      0     838    1419 
   Size:        838     581
 
AvaI C'yCGr_G

Cuts at:      0      66    1419 
   Size:         66    1353
 
BamHI G'GATC_C

Cuts at:      0     849    1419 
   Size:        849     570
 
BanI G'GyrC_C

Cuts at:      0      15    1248    1419 
   Size:         15    1233     171

  Fragments arranged by size: 

                1233     171      15
 
BanII G_rGCy'C

Cuts at:      0    1149    1419 
   Size:       1149     270
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     601    1330    1419 
   Size:        601     729      89

  Fragments arranged by size: 

                 729     601      89
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     924    1419 
   Size:        924     495
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61    1419 
   Size:         61    1358
 
BlpI GC'TnA_GC

Cuts at:      0     124    1175    1419 
   Size:        124    1051     244

  Fragments arranged by size: 

                1051     244     124
 
Bme1580I G_kGCm'C

Cuts at:      0      20    1149    1170    1253    1419 
   Size:         20    1129      21      83     166

  Fragments arranged by size: 

                1129     166      83      21      20
 
BmtI G_CTAG'C

Cuts at:      0    1067    1419 
   Size:       1067     352
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     506     868    1419 
   Size:        466      40     362     551

  Fragments arranged by size: 

                 551     466     362      40
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1064    1419 
   Size:       1064     355
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     769     799    1419 
   Size:        769      30     620

  Fragments arranged by size: 

                 769     620      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     383    1312    1327    1348    1419 
   Size:        383     929      15      21      71

  Fragments arranged by size: 

                 929     383      71      21      15
 
BseYI C'CCAG_C

Cuts at:      0     153    1419 
   Size:        153    1266
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1235    1419 
   Size:       1235     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     590    1419 
   Size:        590     829
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     590    1149    1170    1253    1419 
   Size:         20     570     559      21      83     166

  Fragments arranged by size: 

                 570     559     166      83      21      20
 
BspHI T'CATG_A

Cuts at:      0     590    1419 
   Size:        590     829
 
BsrBI CCG'CTC

Cuts at:      0     937    1419 
   Size:        937     482
 
BsrGI T'GTAC_A

Cuts at:      0     179     921    1419 
   Size:        179     742     498

  Fragments arranged by size: 

                 742     498     179
 
BstEII G'GTnAC_C

Cuts at:      0      48    1419 
   Size:         48    1371
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825    1260    1419 
   Size:        825     435     159

  Fragments arranged by size: 

                 825     435     159
 
BstYI r'GATC_y

Cuts at:      0     849    1419 
   Size:        849     570
 
Bsu36I CC'TnA_GG

Cuts at:      0     659    1419 
   Size:        659     760
 
BtgI C'CryG_G

Cuts at:      0     892    1035    1419 
   Size:        892     143     384

  Fragments arranged by size: 

                 892     384     143
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1154    1419 
   Size:       1154     265
 
BtsI GCAGTG_nn'

Cuts at:      0    1265    1419 
   Size:       1265     154
 
EarI CTCTTCn'nnn_

Cuts at:      0      48    1011    1226    1419 
   Size:         48     963     215     193

  Fragments arranged by size: 

                 963     215     193      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      18    1419 
   Size:         18    1401
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     506     868    1250    1342    1419 
   Size:        466      40     362     382      92      77

  Fragments arranged by size: 

                 466     382     362      92      77      40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1309    1419 
   Size:       1309     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200    1145    1236    1302    1419 
   Size:        200     945      91      66     117

  Fragments arranged by size: 

                 945     200     117      91      66
 
EcoRI G'AATT_C

Cuts at:      0     758     955    1419 
   Size:        758     197     464

  Fragments arranged by size: 

                 758     464     197
 
FspI TGC'GCA

Cuts at:      0     624    1419 
   Size:        624     795
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     468     500     658     690     790     822 
   Size:        130      32     306      32     158      32     100      32

Cuts at:    822    1419 
   Size:        597

  Fragments arranged by size: 

                 597     306     158     130     100      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     973    1419 
   Size:        973     446
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     134    1419 
   Size:        128       6    1285

  Fragments arranged by size: 

                1285     128       6
 
MslI CAynn'nnrTG

Cuts at:      0     470    1419 
   Size:        470     949
 
MspA1I CmG'CkG

Cuts at:      0     985    1419 
   Size:        985     434
 
NcoI C'CATG_G

Cuts at:      0    1035    1419 
   Size:       1035     384
 
NheI G'CTAG_C

Cuts at:      0    1063    1419 
   Size:       1063     356
 
NspI r_CATG'y

Cuts at:      0     672    1419 
   Size:        672     747
 
PasI CC'CwG_GG

Cuts at:      0     613    1419 
   Size:        613     806
 
PciI A'CATG_T

Cuts at:      0     668    1419 
   Size:        668     751
 
PfoI T'CCnGG_A

Cuts at:      0      86     443    1046    1419 
   Size:         86     357     603     373

  Fragments arranged by size: 

                 603     373     357      86
 
PpuMI rG'GwC_Cy

Cuts at:      0     200    1419 
   Size:        200    1219
 
PsiI TTA'TAA

Cuts at:      0     304    1419 
   Size:        304    1115
 
PspOMI G'GGCC_C

Cuts at:      0    1145    1419 
   Size:       1145     274
 
PstI C_TGCA'G

Cuts at:      0     426    1419 
   Size:        426     993
 
SanDI GG'GwC_CC

Cuts at:      0     200    1419 
   Size:        200    1219
 
SapI GCTCTTCn'nnn_

Cuts at:      0      48    1419 
   Size:         48    1371
 
SfcI C'TryA_G

Cuts at:      0     332     422     677    1419 
   Size:        332      90     255     742

  Fragments arranged by size: 

                 742     332     255      90
 
SmaI CCC'GGG

Cuts at:      0      68    1419 
   Size:         68    1351
 
SmlI C'TyrA_G

Cuts at:      0     976    1419 
   Size:        976     443
 
SspI AAT'ATT

Cuts at:      0     775    1419 
   Size:        775     644
 
StyI C'CwwG_G

Cuts at:      0     818    1035    1253    1419 
   Size:        818     217     218     166

  Fragments arranged by size: 

                 818     218     217     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      36    1419 
   Size:         36    1383
 
TatI w'GTAC_w

Cuts at:      0     179     921    1419 
   Size:        179     742     498

  Fragments arranged by size: 

                 742     498     179
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     647     679    1419 
   Size:        647      32     740

  Fragments arranged by size: 

                 740     647      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     826    1419 
   Size:        826     593
 
XmaI C'CCGG_G

Cuts at:      0      66    1419 
   Size:         66    1353
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AhdI     ApaI     ApoI     AseI     AvaI
    BamHI     BanI    BanII     BbsI    BciVI     BglI     BlpI Bme1580I
     BmtI     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BsrBI    BsrGI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     FspI    Hin4I  HindIII     MmeI     MslI   MspA1I     NcoI
     NheI     NspI     PasI     PciI     PfoI    PpuMI     PsiI   PspOMI
     PstI    SanDI     SapI     SfcI     SmaI     SmlI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII    BmgBI     BmrI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoRV     FalI     FseI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
    PflMI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     XbaI     XcmI     XhoI     XmnI     ZraI
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