(Circular) (Six-base) MAPSORT of: pFN21AE0937.seq Check: 6924 from: 1 to: 5989 pFN21AE0937 5989 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2250 3332 1937 Size: 313 1082 4594 Fragments arranged by size: 4594 1082 313 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2655 4052 5436 5704 1632 Size: 1023 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1384 1023 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5989 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AlwNI CAG_nnn'CTG Cuts at: 2035 2047 2503 2561 3732 5304 2035 Size: 12 456 58 1171 1572 2720 Fragments arranged by size: 2720 1572 1171 456 58 12 ApaI G_GGCC'C Cuts at: 2763 3279 2763 Size: 516 5473 ApaLI G'TGCA_C Cuts at: 3218 4046 5202 3218 Size: 828 1156 4005 Fragments arranged by size: 4005 1156 828 ApoI r'AATT_y Cuts at: 1493 2102 3298 3524 3676 5785 1493 Size: 609 1196 226 152 2109 1697 Fragments arranged by size: 2109 1697 1196 609 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2470 2584 2675 2952 2977 3314 1870 Size: 73 527 114 91 277 25 337 4545 Fragments arranged by size: 4545 527 337 277 114 91 73 25 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3310 4765 618 Size: 325 217 627 1523 1455 1842 Fragments arranged by size: 1842 1523 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2763 3279 3308 1391 Size: 1372 516 29 4072 Fragments arranged by size: 4072 1372 516 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5018 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 1903 Size: 34 2243 34 3678 Fragments arranged by size: 3678 2243 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2857 4600 136 Size: 107 122 71 631 605 1185 1743 1525 Fragments arranged by size: 1743 1525 1185 631 605 122 107 71 BglII A'GATC_T Cuts at: 2922 5984 2922 Size: 3062 2927 BlpI GC'TnA_GC Cuts at: 2183 3406 2183 Size: 1223 4766 Bme1580I G_kGCm'C Cuts at: 1790 2763 3222 3279 4050 5206 1790 Size: 973 459 57 771 1156 2573 Fragments arranged by size: 2573 1156 973 771 459 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2544 3720 4678 Size: 416 53 83 399 24 1127 1176 958 Cuts at: 4678 442 Size: 1753 Fragments arranged by size: 1753 1176 1127 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2648 3023 3160 4649 1239 Size: 1409 375 137 1489 2579 Fragments arranged by size: 2579 1489 1409 375 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4114 4979 5277 4114 Size: 865 298 4826 Fragments arranged by size: 4826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2587 2617 2780 Size: 30 165 30 657 30 497 30 163 Cuts at: 2780 2810 1178 Size: 30 4357 Fragments arranged by size: 4357 657 497 165 163 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 1186 Size: 952 5037 BseYI C'CCAG_C Cuts at: 2307 2928 3110 5192 5688 2307 Size: 621 182 2082 496 2608 Fragments arranged by size: 2608 2082 621 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2433 2574 1163 Size: 573 697 141 4578 Fragments arranged by size: 4578 697 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2288 2547 2600 3146 3222 3267 4050 4135 5206 Size: 259 53 546 76 45 783 85 1071 Cuts at: 5206 2288 Size: 3071 Fragments arranged by size: 3071 1071 783 546 259 85 76 53 45 BsmI GAATG_Cn' Cuts at: 3129 3500 3593 3129 Size: 371 93 5525 Fragments arranged by size: 5525 371 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2288 2547 2600 2763 3146 3222 3267 Size: 399 498 259 53 163 383 76 45 Cuts at: 3267 3279 3308 4050 4135 5206 1391 Size: 12 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 383 259 163 85 76 53 45 29 12 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 3878 5608 3878 Size: 1730 4259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2647 3344 844 Size: 1803 697 3489 Fragments arranged by size: 3489 1803 697 BsrBI CCG'CTC Cuts at: 1394 1823 2235 3876 1394 Size: 429 412 1641 3507 Fragments arranged by size: 3507 1641 429 412 BsrDI GCAATG_nn' Cuts at: 65 1248 2068 3156 4484 4658 65 Size: 1183 820 1088 1328 174 1396 Fragments arranged by size: 1396 1328 1183 1088 820 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2642 3052 4633 4820 1700 Size: 110 832 410 1581 187 2869 Fragments arranged by size: 2869 1581 832 410 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 4049 5061 4049 Size: 1012 4977 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 2561 794 Size: 1241 526 4222 Fragments arranged by size: 4222 1241 526 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstYI r'GATC_y Cuts at: 1972 2922 3319 3686 4071 4088 5540 5626 5638 Size: 950 397 367 385 17 1452 86 12 Cuts at: 5638 5984 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 950 397 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2271 2271 Size: 5989 BtgI C'CryG_G Cuts at: 513 1065 2004 2865 3258 513 Size: 552 939 861 393 3244 Fragments arranged by size: 3244 939 861 552 393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5250 Fragments arranged by size: 5250 683 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 2236 2899 Size: 54 1425 50 275 18 235 171 663 Cuts at: 2899 3174 3436 4325 5954 8 Size: 275 262 889 1629 43 Fragments arranged by size: 1629 1425 889 663 275 275 262 235 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 2694 2763 3919 1353 Size: 1278 63 69 1156 3423 Fragments arranged by size: 3423 1278 1156 69 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2648 2655 3023 3160 4052 4649 5436 Size: 393 1016 7 368 137 892 597 787 Cuts at: 5436 5704 1239 Size: 268 1524 Fragments arranged by size: 1524 1016 892 787 597 393 368 268 137 7 EcoO109I rG'GnC_Cy Cuts at: 2680 2759 2956 2999 3275 3276 2680 Size: 79 197 43 276 1 5393 Fragments arranged by size: 5393 276 197 79 43 1 EcoRI G'AATT_C Cuts at: 2102 3298 3676 2102 Size: 1196 378 4415 Fragments arranged by size: 4415 1196 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3202 3202 Size: 5989 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 2579 3059 3213 5136 1678 Size: 901 480 154 1923 2531 Fragments arranged by size: 2531 1923 901 480 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2587 2619 3233 Size: 32 135 32 797 32 186 32 614 Cuts at: 3233 3265 4630 4662 4704 4736 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 797 614 186 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3579 669 Size: 988 281 1395 246 3079 Fragments arranged by size: 3079 1395 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2754 5103 5287 1305 Size: 1449 2349 184 2007 Fragments arranged by size: 2349 2007 1449 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2067 10 Size: 54 1425 343 235 3932 Fragments arranged by size: 3932 1425 343 235 54 MslI CAynn'nnrTG Cuts at: 518 1166 3947 4306 4465 518 Size: 648 2781 359 159 2042 Fragments arranged by size: 2781 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2867 3405 4082 5230 5475 1606 Size: 438 823 538 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 677 538 438 245 NaeI GCC'GGC Cuts at: 2644 3054 2644 Size: 410 5579 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NgoMIV G'CCGG_C Cuts at: 2642 3052 2642 Size: 410 5579 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 2366 2467 3347 4892 1132 Size: 97 1137 101 880 1545 2229 Fragments arranged by size: 2229 1545 1137 880 101 97 PasI CC'CwG_GG Cuts at: 1348 2372 3272 1348 Size: 1024 900 4065 Fragments arranged by size: 4065 1024 900 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 3203 1108 Size: 2095 3894 PfoI T'CCnGG_A Cuts at: 2078 2078 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2956 2999 2956 Size: 43 5946 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PspOMI G'GGCC_C Cuts at: 2759 3275 2759 Size: 516 5473 PstI C_TGCA'G Cuts at: 830 2043 2508 2566 2658 3341 830 Size: 1213 465 58 92 683 3478 Fragments arranged by size: 3478 1213 683 465 92 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2044 2044 Size: 5989 SacII CC_GC'GG Cuts at: 2868 2868 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SanDI GG'GwC_CC Cuts at: 2999 2999 Size: 5989 SapI GCTCTTCn'nnn_ Cuts at: 2694 2694 Size: 5989 SbfI CC_TGCA'GG Cuts at: 2566 3341 2566 Size: 775 5214 ScaI AGT'ACT Cuts at: 4237 5949 4237 Size: 1712 4277 SfcI C'TryA_G Cuts at: 826 1045 2039 2504 2562 2654 3337 4472 5153 Size: 219 994 465 58 92 683 1135 681 Cuts at: 5153 5344 826 Size: 191 1471 Fragments arranged by size: 1471 1135 994 683 681 465 219 191 92 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 2954 3316 1872 Size: 1082 362 4545 Fragments arranged by size: 4545 1082 362 SmlI C'TyrA_G Cuts at: 820 1017 1943 4093 4994 5256 820 Size: 197 926 2150 901 262 1553 Fragments arranged by size: 2150 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 2621 1517 Size: 1104 4885 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 2613 2763 2814 3428 513 Size: 552 939 402 207 150 51 614 3074 Fragments arranged by size: 3074 939 614 552 402 207 150 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 3124 4016 4033 4186 4371 4816 Size: 1255 839 705 892 17 153 185 445 Cuts at: 4816 325 Size: 1498 Fragments arranged by size: 1498 1255 892 839 705 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2460 4235 5947 Size: 274 80 33 51 1443 483 1775 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 1775 1712 1443 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 3136 3168 5949 5981 2060 Size: 32 1044 32 2781 32 2068 Fragments arranged by size: 2781 2068 1044 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2381 1316 Size: 1065 4924 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2770 2341 Size: 429 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 2952 3314 1870 Size: 1082 362 4545 Fragments arranged by size: 4545 1082 362 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI