Restriction map for
pFN21AE0937
(Linear) (Six-base) MAPSORT of: KIEE0937.seq Check: 6381 from: 1 to: 1284
KIEE0937.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 245 1284
Size: 245 1039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1284
Size: 650 634
AlwNI CAG_nnn'CTG
Cuts at: 0 30 42 498 556 1284
Size: 30 12 456 58 728
Fragments arranged by size:
728 456 58 30 12
ApaI G_GGCC'C
Cuts at: 0 758 1274 1284
Size: 758 516 10
Fragments arranged by size:
758 516 10
ApaLI G'TGCA_C
Cuts at: 0 1213 1284
Size: 1213 71
ApoI r'AATT_y
Cuts at: 0 97 1284
Size: 97 1187
AvaI C'yCGr_G
Cuts at: 0 465 579 670 947 972 1284
Size: 465 114 91 277 25 312
Fragments arranged by size:
465 312 277 114 91 25
BanII G_rGCy'C
Cuts at: 0 758 1274 1284
Size: 758 516 10
Fragments arranged by size:
758 516 10
BglI GCCn_nnn'nGGC
Cuts at: 0 852 1284
Size: 852 432
BglII A'GATC_T
Cuts at: 0 917 1284
Size: 917 367
BlpI GC'TnA_GC
Cuts at: 0 178 1284
Size: 178 1106
Bme1580I G_kGCm'C
Cuts at: 0 758 1217 1274 1284
Size: 758 459 57 10
Fragments arranged by size:
758 459 57 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 539 1284
Size: 539 745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1018 1155 1284
Size: 643 375 137 129
Fragments arranged by size:
643 375 137 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 582 612 775 805 1284
Size: 55 30 497 30 163 30 479
Fragments arranged by size:
497 479 163 55 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 1284
Size: 133 1151
BseYI C'CCAG_C
Cuts at: 0 302 923 1105 1284
Size: 302 621 182 179
Fragments arranged by size:
621 302 182 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 569 1284
Size: 428 141 715
Fragments arranged by size:
715 428 141
BsiHKAI G_wGCw'C
Cuts at: 0 283 542 595 1141 1217 1262 1284
Size: 283 259 53 546 76 45 22
Fragments arranged by size:
546 283 259 76 53 45 22
BsmI GAATG_Cn'
Cuts at: 0 1124 1284
Size: 1124 160
Bsp1286I G_dGCh'C
Cuts at: 0 283 542 595 758 1141 1217 1262 1274
Size: 283 259 53 163 383 76 45 12
Cuts at: 1274 1284
Size: 10
Fragments arranged by size:
383 283 259 163 76 53 45 12
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 1284
Size: 642 642
BsrBI CCG'CTC
Cuts at: 0 230 1284
Size: 230 1054
BsrDI GCAATG_nn'
Cuts at: 0 63 1151 1284
Size: 63 1088 133
Fragments arranged by size:
1088 133 63
BsrFI r'CCGG_y
Cuts at: 0 637 1047 1284
Size: 637 410 237
Fragments arranged by size:
637 410 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 556 1284
Size: 30 526 728
Fragments arranged by size:
728 526 30
BstYI r'GATC_y
Cuts at: 0 917 1284
Size: 917 367
Bsu36I CC'TnA_GG
Cuts at: 0 266 1284
Size: 266 1018
BtgI C'CryG_G
Cuts at: 0 860 1253 1284
Size: 860 393 31
Fragments arranged by size:
860 393 31
EaeI y'GGCC_r
Cuts at: 0 60 231 894 1169 1284
Size: 60 171 663 275 115
Fragments arranged by size:
663 275 171 115 60
EarI CTCTTCn'nnn_
Cuts at: 0 626 689 758 1284
Size: 626 63 69 526
Fragments arranged by size:
626 526 69 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 650 1018 1155 1284
Size: 643 7 368 137 129
Fragments arranged by size:
643 368 137 129 7
EcoO109I rG'GnC_Cy
Cuts at: 0 675 754 951 994 1270 1271 1284
Size: 675 79 197 43 276 1 13
Fragments arranged by size:
675 276 197 79 43 13 1
EcoRI G'AATT_C
Cuts at: 0 97 1284
Size: 97 1187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1197 1284
Size: 1197 87
HaeII r_GCGC'y
Cuts at: 0 574 1054 1208 1284
Size: 574 480 154 76
Fragments arranged by size:
574 480 154 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 582 614 1228 1260 1284
Size: 364 32 186 32 614 32 24
Fragments arranged by size:
614 364 186 32 32 32 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1284
Size: 749 535
MscI TGG'CCA
Cuts at: 0 62 1284
Size: 62 1222
MspA1I CmG'CkG
Cuts at: 0 39 862 1284
Size: 39 823 422
Fragments arranged by size:
823 422 39
NaeI GCC'GGC
Cuts at: 0 639 1049 1284
Size: 639 410 235
Fragments arranged by size:
639 410 235
NgoMIV G'CCGG_C
Cuts at: 0 637 1047 1284
Size: 637 410 237
Fragments arranged by size:
637 410 237
NspI r_CATG'y
Cuts at: 0 361 462 1284
Size: 361 101 822
Fragments arranged by size:
822 361 101
PasI CC'CwG_GG
Cuts at: 0 367 1267 1284
Size: 367 900 17
Fragments arranged by size:
900 367 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 1198 1284
Size: 1198 86
PfoI T'CCnGG_A
Cuts at: 0 73 1284
Size: 73 1211
PpuMI rG'GwC_Cy
Cuts at: 0 951 994 1284
Size: 951 43 290
Fragments arranged by size:
951 290 43
PspOMI G'GGCC_C
Cuts at: 0 754 1270 1284
Size: 754 516 14
Fragments arranged by size:
754 516 14
PstI C_TGCA'G
Cuts at: 0 38 503 561 653 1284
Size: 38 465 58 92 631
Fragments arranged by size:
631 465 92 58 38
PvuII CAG'CTG
Cuts at: 0 39 1284
Size: 39 1245
SacII CC_GC'GG
Cuts at: 0 863 1284
Size: 863 421
SanDI GG'GwC_CC
Cuts at: 0 994 1284
Size: 994 290
SapI GCTCTTCn'nnn_
Cuts at: 0 689 1284
Size: 689 595
SbfI CC_TGCA'GG
Cuts at: 0 561 1284
Size: 561 723
SfcI C'TryA_G
Cuts at: 0 34 499 557 649 1284
Size: 34 465 58 92 635
Fragments arranged by size:
635 465 92 58 34
SmaI CCC'GGG
Cuts at: 0 949 1284
Size: 949 335
StuI AGG'CCT
Cuts at: 0 616 1284
Size: 616 668
StyI C'CwwG_G
Cuts at: 0 401 608 758 809 1284
Size: 401 207 150 51 475
Fragments arranged by size:
475 401 207 150 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 1119 1284
Size: 414 705 165
Fragments arranged by size:
705 414 165
TatI w'GTAC_w
Cuts at: 0 455 1284
Size: 455 829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 1131 1163 1284
Size: 55 32 1044 32 121
Fragments arranged by size:
1044 121 55 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 376 1284
Size: 376 908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 765 1284
Size: 336 429 519
Fragments arranged by size:
519 429 336
XmaI C'CCGG_G
Cuts at: 0 947 1284
Size: 947 337
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApaLI ApoI AvaI BanII
BglI BglII BlpI Bme1580I BmrI BpmI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstYI Bsu36I BtgI EaeI EarI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I MmeI MscI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacII SanDI SapI SbfI SfcI SmaI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmnI ZraI