(Circular) (Six-base) MAPSORT of: pFN21AE0931.seq Check: 2740 from: 1 to: 5875 pFN21AE0931 5875 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 2396 2799 4002 4375 1563 Size: 833 403 1203 373 3063 Fragments arranged by size: 3063 1203 833 403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2198 2481 2705 3048 3938 5322 5590 Size: 514 52 283 224 343 890 1384 268 Cuts at: 5590 1632 Size: 1917 Fragments arranged by size: 1917 1384 890 514 343 283 268 224 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 2147 2194 2814 2827 3618 5190 2147 Size: 47 620 13 791 1572 2832 Fragments arranged by size: 2832 1572 791 620 47 13 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2130 3200 1870 Size: 73 187 1070 4545 Fragments arranged by size: 4545 1070 187 73 BamHI G'GATC_C Cuts at: 2321 2452 2566 2733 3205 3572 2321 Size: 131 114 167 472 367 4624 Fragments arranged by size: 4624 472 367 167 131 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3196 4651 618 Size: 325 217 627 1409 1455 1842 Fragments arranged by size: 1842 1455 1409 627 325 217 BanII G_rGCy'C Cuts at: 1391 2721 3194 1391 Size: 1330 473 4072 Fragments arranged by size: 4072 1330 473 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2627 2661 4066 4100 1903 Size: 34 690 34 1405 34 3678 Fragments arranged by size: 3678 1405 690 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 3936 5092 1790 Size: 2146 1156 2573 Fragments arranged by size: 2573 2146 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2262 4535 1239 Size: 1023 2273 2579 Fragments arranged by size: 2579 2273 1023 Bpu10I CC'TnA_GC Cuts at: 2608 2608 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2366 2713 3123 4000 4865 5163 2366 Size: 347 410 877 865 298 3078 Fragments arranged by size: 3078 877 865 410 347 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3039 3069 1178 Size: 30 165 30 1636 30 3984 Fragments arranged by size: 3984 1636 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2067 2079 3131 1186 Size: 881 12 1052 3930 Fragments arranged by size: 3930 1052 881 12 BseYI C'CCAG_C Cuts at: 5078 5574 5078 Size: 496 5379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2449 3936 4021 5092 2449 Size: 1487 85 1071 3232 Fragments arranged by size: 3232 1487 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2449 2721 3194 3936 4021 5092 1391 Size: 399 659 272 473 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 659 473 399 272 85 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2907 3764 5494 2907 Size: 857 1730 3288 Fragments arranged by size: 3288 1730 857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 2557 4679 2557 Size: 2122 3753 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2321 2452 2566 2733 2988 3205 3572 3957 Size: 349 131 114 167 255 217 367 385 Cuts at: 3957 3974 5426 5512 5524 5870 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 349 346 255 217 167 131 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2023 513 Size: 552 958 4365 Fragments arranged by size: 4365 958 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 3391 4185 4205 3391 Size: 794 20 5061 Fragments arranged by size: 5061 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2359 2975 2359 Size: 616 5259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2995 3322 4211 Size: 54 1425 50 275 18 1165 327 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1165 889 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 3108 3805 1353 Size: 821 934 697 3423 Fragments arranged by size: 3423 934 821 697 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2198 2262 2481 2705 3048 3938 Size: 393 514 52 64 219 224 343 890 Cuts at: 3938 4535 5322 5590 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 514 393 343 268 224 219 64 52 EcoNI CCTnn'n_nnAGG Cuts at: 2996 2996 Size: 5875 EcoO109I rG'GnC_Cy Cuts at: 2076 2191 2076 Size: 115 5760 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 EcoRV GAT'ATC Cuts at: 2587 2587 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2041 2815 5022 1678 Size: 363 774 2207 2531 Fragments arranged by size: 2531 2207 774 363 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2248 2260 2280 2292 4516 Size: 32 135 32 676 12 20 12 2224 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2224 676 135 42 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 2644 4989 5173 1305 Size: 1074 265 2345 184 2007 Fragments arranged by size: 2345 2007 1074 265 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2997 10 Size: 54 1425 343 1165 2888 Fragments arranged by size: 2888 1425 1165 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2906 3833 4192 4351 518 Size: 648 1740 927 359 159 2042 Fragments arranged by size: 2042 1740 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2631 3291 3968 5116 5361 1606 Size: 1025 660 677 1148 245 2120 Fragments arranged by size: 2120 1148 1025 677 660 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5323 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2627 3233 4778 1132 Size: 97 1398 606 1545 2229 Fragments arranged by size: 2229 1545 1398 606 97 PasI CC'CwG_GG Cuts at: 1348 2416 1348 Size: 1068 4807 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2191 Size: 5875 PshAI GACnn'nnGTC Cuts at: 1613 2954 1613 Size: 1341 4534 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 2836 3227 830 Size: 2006 391 3478 Fragments arranged by size: 3478 2006 391 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SexAI A'CCwGG_T Cuts at: 2367 2367 Size: 5875 SfcI C'TryA_G Cuts at: 826 1045 2346 2517 2832 3223 4358 5039 5230 Size: 219 1301 171 315 391 1135 681 191 Cuts at: 5230 826 Size: 1471 Fragments arranged by size: 1471 1301 1135 681 391 315 219 191 171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 2132 3202 1872 Size: 260 1070 4545 Fragments arranged by size: 4545 1070 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2381 2692 3138 3979 4880 5142 Size: 197 926 438 311 446 841 901 262 Cuts at: 5142 820 Size: 1553 Fragments arranged by size: 1553 926 901 841 446 438 311 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 2171 1517 Size: 654 5221 StyI C'CwwG_G Cuts at: 513 1065 2992 3314 513 Size: 552 1927 322 3074 Fragments arranged by size: 3074 1927 552 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 3902 3919 4072 4257 4702 325 Size: 1255 983 1339 17 153 185 445 1498 Fragments arranged by size: 1498 1339 1255 983 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2896 4121 5833 Size: 274 80 33 51 1443 919 1225 1712 Cuts at: 5833 96 Size: 138 Fragments arranged by size: 1712 1443 1225 919 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2343 2375 2718 2750 5835 5867 2343 Size: 32 343 32 3085 32 2351 Fragments arranged by size: 3085 2351 343 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2930 1316 Size: 1614 4261 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2785 2785 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 2130 3200 1870 Size: 260 1070 4545 Fragments arranged by size: 4545 1070 260 XmnI GAAnn'nnTTC Cuts at: 2307 4004 2307 Size: 1697 4178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI