Restriction map for
pFN21AE0930
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AvaI 1082 CCCGAG C^[CT]CG[GA]G
BclI 342 TGATCA T^GATCA
BfaI 140 CTAG C^TAG
BsaI 1090 GAGACC GGTCTC(1/5)
BsgI 68 CTGCAC GTGCAG(16/14)
BsoBI 1082 CCCGAG C^[CT]CG[GA]G
BsrFI 612 GCCGGC [GA]^CCGG[CT]
Cfr10I 612 GCCGGC [GA]^CCGG[CT]
DraI 378 TTTAAA TTT^AAA
EarI 362 GAAGAG CTCTTC(1/4)
EcoRV 868 GATATC GAT^ATC
EcoT14I 981 CCAAGG C^C[AT][AT]GG
FbaI 342 TGATCA T^GATCA
HapII 613 CCGG C^CGG
HpaII 613 CCGG C^CGG
Ksp632I 362 GAAGAG CTCTTC(1/4)
MaeI 140 CTAG C^TAG
MspI 613 CCGG C^CGG
NaeI 612 GCCGGC GCC^GGC
NgoMIV 612 GCCGGC G^CCGGC
NlaIII 742 CATG CATG^
ScaI 756 AGTACT AGT^ACT
SexAI 566 ACCTGGT A^CC[AT]GGT
SfcI 544 CTACAG C^T[GA][CT]AG
SmlI 210 CTTGAG C^T[CT][GA]AG
StyI 981 CCAAGG C^C[AT][AT]GG
TaqI 582 TCGA T^CGA
TthHB8I 582 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 145 AAATTT [GA]^AATT[CT]
933 AAATTT
AlwI 738 GATCC GGATC(4/5)
813 GGATC
AlwNI 469 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
1024 CAGTTCCTG
ApaI 521 GGGCCC GGGCC^C
606 GGGCCC
ApoI 145 AAATTT [GA]^AATT[CT]
933 AAATTT
AvaII 117 GGTCC G^G[AT]CC
880 GGACC
BanI 837 GGTGCC G^G[CT][GA]CC
952 GGTGCC
BanII 521 GGGCCC G[GA]GC[CT]^C
606 GGGCCC
BmyI 521 GGGCCC G[AGT]GC[ACT]^C
606 GGGCCC
BseRI 1143 CTCCTC GAGGAG(10/8)
1132 GAGGAG
Bsp1286I 521 GGGCCC G[AGT]GC[ACT]^C
606 GGGCCC
BsrDI 76 GCAATG GCAATG(2/0)
394 GCAATG
BstF5I 767 GGATG GGATG(2/0)
996 GGATG
BstYI 737 AGATCC [GA]^GATC[CT]
813 GGATCT
FauI 609 CCCGC CCCGC(4/6)
519 GCGGG
FokI 767 GGATG GGATG(9/13)
996 GGATG
MflI 737 AGATCC [GA]^GATC[CT]
813 GGATCT
MseI 379 TTAA T^TAA
892 TTAA
MspA1I 526 CAGCTG C[AC]G^C[GT]G
634 CAGCTG
PpuMI 116 GGGTCCC [GA]G^G[AT]CC[CT]
879 AGGACCT
PvuII 526 CAGCTG CAG^CTG
634 CAGCTG
Tru9I 379 TTAA T^TAA
892 TTAA
Tsp45I 695 GTGAC ^GT[GC]AC
1043 GTCAC
XhoII 737 AGATCC [GA]^GATC[CT]
813 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BsaJI BsmAI BsmFI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp509I
TspRI