Restriction map for
pFN21AE0927
(Circular) (Six-base) MAPSORT of: pFN21AE0927.seq Check: 6739 from: 1 to: 5794
pFN21AE0927 5794 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 2328
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 2979 3054 3857 5241 5509 1632
Size: 753 594 75 803 1384 268 1917
Fragments arranged by size:
1917 1384 803 753 594 268 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 2593 4693 5670 1203
Size: 1390 2100 977 1327
Fragments arranged by size:
2100 1390 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2600 1184
Size: 1416 4378
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2034 2066 2034
Size: 32 5762
AlwNI CAG_nnn'CTG
Cuts at: 2413 3077 3537 5109 2413
Size: 664 460 1572 3098
Fragments arranged by size:
3098 1572 664 460
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 3103 3329 3481 5590 1493
Size: 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
BamHI G'GATC_C
Cuts at: 2063 3124 3491 2063
Size: 1061 367 4366
Fragments arranged by size:
4366 1061 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2813 3115 4570 618
Size: 325 217 627 1026 302 1455 1842
Fragments arranged by size:
1842 1455 1026 627 325 302 217
BanII G_rGCy'C
Cuts at: 1391 2663 3113 1391
Size: 1272 450 4072
Fragments arranged by size:
4072 1272 450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2742 928
Size: 971 843 3980
Fragments arranged by size:
3980 971 843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2784 5789 2784
Size: 3005 2789
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 3855 5011 1790
Size: 2065 1156 2573
Fragments arranged by size:
2573 2065 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2574 4454 1239
Size: 1335 1880 2579
Fragments arranged by size:
2579 1880 1335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2681 3919 4784 5082 2681
Size: 1238 865 298 3393
Fragments arranged by size:
3393 1238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 4457 882
Size: 617 1056 1902 2219
Fragments arranged by size:
2219 1902 1056 617
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3601 3983 4618
Size: 53 83 186 936 482 1586 382 635
Cuts at: 4618 275
Size: 1451
Fragments arranged by size:
1586 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4227 4625 4899 5046 5457 4227
Size: 398 274 147 411 4564
Fragments arranged by size:
4564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2291 2321 1178
Size: 30 165 30 888 30 4651
Fragments arranged by size:
4651 888 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2978 1186
Size: 930 862 4002
Fragments arranged by size:
4002 930 862
BseYI C'CCAG_C
Cuts at: 2119 2710 4997 5493 2119
Size: 591 2287 496 2420
Fragments arranged by size:
2420 2287 591 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2667 3244 4005 5033 1540
Size: 275 186 666 577 761 1028 2301
Fragments arranged by size:
2301 1028 761 666 577 275 186
BsiHKAI G_wGCw'C
Cuts at: 3855 3940 5011 3855
Size: 85 1071 4638
Fragments arranged by size:
4638 1071 85
BsmI GAATG_Cn'
Cuts at: 2628 3305 3398 2628
Size: 677 93 5024
Fragments arranged by size:
5024 677 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2663 3113 3855 3940 5011 1391
Size: 399 873 450 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 450 399 85
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 3048 3149 844
Size: 1484 720 101 3489
Fragments arranged by size:
3489 1484 720 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2170 2625 2707 2896 3681 1394
Size: 429 347 455 82 189 785 3507
Fragments arranged by size:
3507 785 455 429 347 189 82
BsrDI GCAATG_nn'
Cuts at: 65 1248 4289 4463 65
Size: 1183 3041 174 1396
Fragments arranged by size:
3041 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4438 4625 1700
Size: 110 2628 187 2869
Fragments arranged by size:
2869 2628 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2352 2500 2548 2352
Size: 148 48 5598
Fragments arranged by size:
5598 148 48
BstYI r'GATC_y
Cuts at: 1972 2063 2784 3124 3491 3876 3893 5345 5431
Size: 91 721 340 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 721 385 367 346 340 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2532 513
Size: 552 939 528 3775
Fragments arranged by size:
3775 939 552 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 4104 4124 3310
Size: 794 20 4980
Fragments arranged by size:
4980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 2971 3098 2971
Size: 127 5667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2438 4801 809
Size: 507 1122 2363 1802
Fragments arranged by size:
2363 1802 1122 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2325 2664
Size: 54 1425 50 275 18 360 135 339
Cuts at: 2664 2991 3241 4130 5759 8
Size: 327 250 889 1629 43
Fragments arranged by size:
1629 1425 889 360 339 327 275 250
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2664 3241 1537
Size: 275 852 577 4090
Fragments arranged by size:
4090 852 577 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2615 2955 3724 1353
Size: 1262 340 769 3423
Fragments arranged by size:
3423 1262 769 340
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 2574 2979 3054 3857 4454 5241
Size: 393 753 189 405 75 803 597 787
Cuts at: 5241 5509 1239
Size: 268 1524
Fragments arranged by size:
1524 803 787 753 597 405 393 268
189 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2417 2417
Size: 5794
EcoO109I rG'GnC_Cy
Cuts at: 2371 2410 2743 2371
Size: 39 333 5422
Fragments arranged by size:
5422 333 39
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 4941 1678
Size: 3263 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4435 4467 4509 4541 1373
Size: 32 135 32 2863 32 42 32 2626
Fragments arranged by size:
2863 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4908 5092 1305
Size: 3603 184 2007
Fragments arranged by size:
3603 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2327 10
Size: 54 1425 343 360 135 3477
Fragments arranged by size:
3477 1425 360 343 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 3752 4111 4270 518
Size: 648 990 1596 359 159 2042
Fragments arranged by size:
2042 1596 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3210 3887 5035 5280 1606
Size: 1604 677 1148 245 2120
Fragments arranged by size:
2120 1604 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 2051 2212 2470 2597 2776 2840 3152
Size: 97 822 161 258 127 179 64 312
Cuts at: 3152 4697 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 822 312 258 179 161 127
97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 2593 4693 2593
Size: 2100 3694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 1108
Size: 1623 4171
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2371
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 2751 3364 2751
Size: 613 5181
PstI C_TGCA'G
Cuts at: 830 2418 3082 3146 830
Size: 1588 664 64 3478
Fragments arranged by size:
3478 1588 664 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SapI GCTCTTCn'nnn_
Cuts at: 2615 2615
Size: 5794
SbfI CC_TGCA'GG
Cuts at: 2418 3146 2418
Size: 728 5066
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2414 3078 3142 4277 4958 5149 826
Size: 219 1369 664 64 1135 681 191 1471
Fragments arranged by size:
1471 1369 1135 681 664 219 191 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 3898 4799 5061 820
Size: 197 926 753 1202 901 262 1553
Fragments arranged by size:
1553 1202 926 901 753 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 2470 2840 3152 1132
Size: 1338 370 312 3774
Fragments arranged by size:
3774 1338 370 312
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 2603 1517
Size: 1086 4708
StyI C'CwwG_G
Cuts at: 513 1065 2004 2493 3233 513
Size: 552 939 489 740 3074
Fragments arranged by size:
3074 939 740 552 489
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3821 3838 3991 4176 4621 325
Size: 1255 2241 17 153 185 445 1498
Fragments arranged by size:
2241 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5754 5786 5754
Size: 32 5762
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2335 2944 2284
Size: 51 609 5134
Fragments arranged by size:
5134 609 51
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI