Restriction map for pFN21AE0927
 (Linear) (Six-base) MAPSORT of: KIEE0927.seq  Check: 606  from: 1  to: 1089



KIEE0927.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     323    1089 
   Size:        323     766
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     974    1049    1089 
   Size:        380     594      75      40

  Fragments arranged by size: 

                 594     380      75      40
 
AflIII A'CryG_T

Cuts at:      0     588    1089 
   Size:        588     501
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     595    1089 
   Size:        595     494
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29      61    1089 
   Size:         29      32    1028

  Fragments arranged by size: 

                1028      32      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     408    1072    1089 
   Size:        408     664      17

  Fragments arranged by size: 

                 664     408      17
 
BamHI G'GATC_C

Cuts at:      0      58    1089 
   Size:         58    1031
 
BanI G'GyrC_C

Cuts at:      0     808    1089 
   Size:        808     281
 
BanII G_rGCy'C

Cuts at:      0     658    1089 
   Size:        658     431
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     737    1089 
   Size:        737     352
 
BglII A'GATC_T

Cuts at:      0     779    1089 
   Size:        779     310
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     672    1089 
   Size:        672     417
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     569    1089 
   Size:        569     520
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1089 
   Size:        676     413
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     550    1089 
   Size:        550     539
 
BsaHI Gr'CG_yC

Cuts at:      0      10    1089 
   Size:         10    1079
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     286     316    1089 
   Size:        286      30     773

  Fragments arranged by size: 

                 773     286      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     111     973    1089 
   Size:        111     862     116

  Fragments arranged by size: 

                 862     116     111
 
BseYI C'CCAG_C

Cuts at:      0     114     705    1089 
   Size:        114     591     384

  Fragments arranged by size: 

                 591     384     114
 
BsiEI CG_ry'CG

Cuts at:      0     662    1089 
   Size:        662     427
 
BsmI GAATG_Cn'

Cuts at:      0     623    1089 
   Size:        623     466
 
Bsp1286I G_dGCh'C

Cuts at:      0     658    1089 
   Size:        658     431
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     323    1043    1089 
   Size:        323     720      46

  Fragments arranged by size: 

                 720     323      46
 
BsrBI CCG'CTC

Cuts at:      0     165     620     702     891    1089 
   Size:        165     455      82     189     198

  Fragments arranged by size: 

                 455     198     189     165      82
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     347     495     543    1089 
   Size:        347     148      48     546

  Fragments arranged by size: 

                 546     347     148      48
 
BstYI r'GATC_y

Cuts at:      0      58     779    1089 
   Size:         58     721     310

  Fragments arranged by size: 

                 721     310      58
 
BtgI C'CryG_G

Cuts at:      0     527    1089 
   Size:        527     562
 
DraI TTT'AAA

Cuts at:      0     966    1089 
   Size:        966     123
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     433    1089 
   Size:        433     656
 
EaeI y'GGCC_r

Cuts at:      0     185     320     659     986    1089 
   Size:        185     135     339     327     103

  Fragments arranged by size: 

                 339     327     185     135     103
 
EagI C'GGCC_G

Cuts at:      0     659    1089 
   Size:        659     430
 
EarI CTCTTCn'nnn_

Cuts at:      0     610     950    1089 
   Size:        610     340     139

  Fragments arranged by size: 

                 610     340     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     569     974    1049    1089 
   Size:        380     189     405      75      40

  Fragments arranged by size: 

                 405     380     189      75      40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     412    1089 
   Size:        412     677
 
EcoO109I rG'GnC_Cy

Cuts at:      0     366     405     738    1089 
   Size:        366      39     333     351

  Fragments arranged by size: 

                 366     351     333      39
 
MscI TGG'CCA

Cuts at:      0     187     322    1089 
   Size:        187     135     767

  Fragments arranged by size: 

                 767     187     135
 
MslI CAynn'nnrTG

Cuts at:      0     151    1089 
   Size:        151     938
 
NspI r_CATG'y

Cuts at:      0      46     207     465     592     771     835    1089 
   Size:         46     161     258     127     179      64     254

  Fragments arranged by size: 

                 258     254     179     161     127      64      46
 
PciI A'CATG_T

Cuts at:      0     588    1089 
   Size:        588     501
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     726    1089 
   Size:        726     363
 
PpuMI rG'GwC_Cy

Cuts at:      0     366    1089 
   Size:        366     723
 
PsiI TTA'TAA

Cuts at:      0     746    1089 
   Size:        746     343
 
PstI C_TGCA'G

Cuts at:      0     413    1077    1089 
   Size:        413     664      12

  Fragments arranged by size: 

                 664     413      12
 
SapI GCTCTTCn'nnn_

Cuts at:      0     610    1089 
   Size:        610     479
 
SbfI CC_TGCA'GG

Cuts at:      0     413    1089 
   Size:        413     676
 
SfcI C'TryA_G

Cuts at:      0     409    1073    1089 
   Size:        409     664      16

  Fragments arranged by size: 

                 664     409      16
 
SmlI C'TyrA_G

Cuts at:      0     691    1089 
   Size:        691     398
 
SphI G_CATG'C

Cuts at:      0     465     835    1089 
   Size:        465     370     254

  Fragments arranged by size: 

                 465     370     254
 
StuI AGG'CCT

Cuts at:      0     598    1089 
   Size:        598     491
 
StyI C'CwwG_G

Cuts at:      0     488    1089 
   Size:        488     601
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279     330     939    1089 
   Size:        279      51     609     150

  Fragments arranged by size: 

                 609     279     150      51
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     AloI    AlwNI    BamHI     BanI
    BanII     BbsI    BglII     BplI     BpmI    BpuEI     BsaI    BsaHI
    BsaXI    BseRI    BseYI    BsiEI     BsmI Bsp1286I    BspMI    BsrBI
    BstXI    BstYI     BtgI     DraI     DrdI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I     MscI     MslI     NspI     PciI    PflMI
    PpuMI     PsiI     PstI     SapI     SbfI     SfcI     SmlI     SphI
     StuI     StyI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BsaAI
    BsaBI    BsaWI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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