Restriction map for
pFN21AE0924
(Linear) (Six-base) MAPSORT of: KIEE0924.seq Check: 1987 from: 1 to: 1029
KIEE0924.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
Acc65I G'GTAC_C
Cuts at: 0 466 1029
Size: 466 563
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 323 1029
Size: 268 55 706
Fragments arranged by size:
706 268 55
AflII C'TTAA_G
Cuts at: 0 115 1029
Size: 115 914
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 523 555 1029
Size: 523 32 474
Fragments arranged by size:
523 474 32
AlwNI CAG_nnn'CTG
Cuts at: 0 705 990 1029
Size: 705 285 39
Fragments arranged by size:
705 285 39
ApaLI G'TGCA_C
Cuts at: 0 390 1029
Size: 390 639
ApoI r'AATT_y
Cuts at: 0 909 1029
Size: 909 120
AvaI C'yCGr_G
Cuts at: 0 461 1029
Size: 461 568
AvrII C'CTAG_G
Cuts at: 0 147 1029
Size: 147 882
BamHI G'GATC_C
Cuts at: 0 110 1029
Size: 110 919
BanI G'GyrC_C
Cuts at: 0 466 1029
Size: 466 563
BanII G_rGCy'C
Cuts at: 0 20 1029
Size: 20 1009
BciVI GTATCCnnnnn_n'
Cuts at: 0 42 566 1029
Size: 42 524 463
Fragments arranged by size:
524 463 42
Bme1580I G_kGCm'C
Cuts at: 0 394 767 1029
Size: 394 373 262
Fragments arranged by size:
394 373 262
BmrI ACTGGGnnnn_n'
Cuts at: 0 326 805 1029
Size: 326 479 224
Fragments arranged by size:
479 326 224
BmtI G_CTAG'C
Cuts at: 0 76 1029
Size: 76 953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 550 1029
Size: 407 143 479
Fragments arranged by size:
479 407 143
Bpu10I CC'TnA_GC
Cuts at: 0 400 1029
Size: 400 629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 859 1029
Size: 652 207 170
Fragments arranged by size:
652 207 170
BsaHI Gr'CG_yC
Cuts at: 0 651 1029
Size: 651 378
BsaWI w'CCGG_w
Cuts at: 0 488 1029
Size: 488 541
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 523 553 1029
Size: 523 30 476
Fragments arranged by size:
523 476 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 330 1029
Size: 330 699
BseYI C'CCAG_C
Cuts at: 0 242 821 884 1029
Size: 242 579 63 145
Fragments arranged by size:
579 242 145 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1029
Size: 725 304
BsiHKAI G_wGCw'C
Cuts at: 0 394 1029
Size: 394 635
BsmI GAATG_Cn'
Cuts at: 0 92 1029
Size: 92 937
Bsp1286I G_dGCh'C
Cuts at: 0 20 394 767 1029
Size: 20 374 373 262
Fragments arranged by size:
374 373 262 20
BsrFI r'CCGG_y
Cuts at: 0 32 481 1029
Size: 32 449 548
Fragments arranged by size:
548 449 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 66 1029
Size: 66 963
BstEII G'GTnAC_C
Cuts at: 0 404 1029
Size: 404 625
BstYI r'GATC_y
Cuts at: 0 110 143 1029
Size: 110 33 886
Fragments arranged by size:
886 110 33
Bsu36I CC'TnA_GG
Cuts at: 0 605 1029
Size: 605 424
BtgI C'CryG_G
Cuts at: 0 205 573 1029
Size: 205 368 456
Fragments arranged by size:
456 368 205
BtsI GCAGTG_nn'
Cuts at: 0 765 1029
Size: 765 264
EaeI y'GGCC_r
Cuts at: 0 30 483 965 1029
Size: 30 453 482 64
Fragments arranged by size:
482 453 64 30
EarI CTCTTCn'nnn_
Cuts at: 0 633 1029
Size: 633 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 323 407 550 1029
Size: 268 55 84 143 479
Fragments arranged by size:
479 268 143 84 55
EcoO109I rG'GnC_Cy
Cuts at: 0 799 987 1029
Size: 799 188 42
Fragments arranged by size:
799 188 42
EcoRI G'AATT_C
Cuts at: 0 909 1029
Size: 909 120
FseI GG_CCGG'CC
Cuts at: 0 36 1029
Size: 36 993
KpnI G_GTAC'C
Cuts at: 0 470 1029
Size: 470 559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 656 1029
Size: 309 347 373
Fragments arranged by size:
373 347 309
MscI TGG'CCA
Cuts at: 0 967 1029
Size: 967 62
MspA1I CmG'CkG
Cuts at: 0 246 412 427 684 816 1029
Size: 246 166 15 257 132 213
Fragments arranged by size:
257 246 213 166 132 15
NaeI GCC'GGC
Cuts at: 0 34 1029
Size: 34 995
NgoMIV G'CCGG_C
Cuts at: 0 32 1029
Size: 32 997
NheI G'CTAG_C
Cuts at: 0 72 1029
Size: 72 957
PasI CC'CwG_GG
Cuts at: 0 475 699 1029
Size: 475 224 330
Fragments arranged by size:
475 330 224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 1029
Size: 523 32 474
Fragments arranged by size:
523 474 32
PpuMI rG'GwC_Cy
Cuts at: 0 799 1029
Size: 799 230
PvuII CAG'CTG
Cuts at: 0 246 412 427 684 816 1029
Size: 246 166 15 257 132 213
Fragments arranged by size:
257 246 213 166 132 15
SanDI GG'GwC_CC
Cuts at: 0 799 1029
Size: 799 230
SapI GCTCTTCn'nnn_
Cuts at: 0 633 1029
Size: 633 396
SmaI CCC'GGG
Cuts at: 0 463 1029
Size: 463 566
SmlI C'TyrA_G
Cuts at: 0 115 667 874 1029
Size: 115 552 207 155
Fragments arranged by size:
552 207 155 115
StyI C'CwwG_G
Cuts at: 0 147 715 1029
Size: 147 568 314
Fragments arranged by size:
568 314 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 488 1029
Size: 331 157 541
Fragments arranged by size:
541 331 157
XmaI C'CCGG_G
Cuts at: 0 461 1029
Size: 461 568
XmnI GAAnn'nnTTC
Cuts at: 0 538 1029
Size: 538 491
Enzymes that do cut:
Acc65I AcuI AflII AloI AlwNI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BciVI Bme1580I BmrI BmtI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BsrFI BstAPI BstEII BstYI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRI
FseI KpnI MmeI MscI MspA1I NaeI NgoMIV NheI
PasI PpiI PpuMI PvuII SanDI SapI SmaI SmlI
StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI BaeI BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BplI BsaI BsaAI BsaBI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MslI NarI NcoI NdeI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI