Restriction map for
pFN21AE0915
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaHI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspI PacI PaeR7I PflFI PflMI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 444 GTAC GT^AC
AgeI 33 ACCGGT A^CCGGT
AlwNI 333 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 163 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 91 GGTCC G^G[AT]CC
BamHI 728 GGATCC G^GATCC
BbrPI 709 CACGTG CAC^GTG
BglII 75 AGATCT A^GATCT
BmyI 468 GGGCAC G[AGT]GC[ACT]^C
BsaBI 279 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 24 GAGACC GGTCTC(1/5)
BsmFI 142 GTCCC GGGAC(10/14)
Bsp1286I 468 GGGCAC G[AGT]GC[ACT]^C
BspMI 296 ACCTGC ACCTGC(4/8)
BsrFI 33 ACCGGT [GA]^CCGG[CT]
BsrI 740 CCAGT ACTGG(1/-1)
BstBI 380 TTCGAA TT^CGAA
BstF5I 720 GGATG GGATG(2/0)
BstXI 11 CCAGCGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 33 ACCGGT [GA]^CCGG[CT]
DraII 498 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 59 GACTTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 122 TGGCCG [CT]^GGCC[GA]
EarI 148 GAAGAG CTCTTC(1/4)
EcoO109I 498 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 578 ATGCAT ATGCA^T
FokI 720 GGATG GGATG(9/13)
HaeII 3 GGCGCT [GA]GCGC^[CT]
HincII 697 GTCAAC GT[CT]^[GA]AC
HindII 697 GTCAAC GT[CT]^[GA]AC
Ksp632I 148 GAAGAG CTCTTC(1/4)
MaeIII 743 GTGAC ^GT[ACGT]AC
MamI 279 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 581 CATATG CA^TATG
NsiI 578 ATGCAT ATGCA^T
NspV 380 TTCGAA TT^CGAA
PinAI 33 ACCGGT A^CCGGT
PmaCI 709 CACGTG CAC^GTG
PmlI 709 CACGTG CAC^GTG
RsaI 444 GTAC GT^AC
SapI 148 GAAGAGC GCTCTTC(1/4)
SfuI 380 TTCGAA TT^CGAA
StuI 629 AGGCCT AGG^CCT
Tsp45I 743 GTGAC ^GT[GC]AC
XcmI 740 CCAGTGACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 163 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 46 CGCG CG^CG
126 CGCG
BanI 304 GGCACC G^G[CT][GA]CC
469 GGCACC
BfaI 88 CTAG C^TAG
301 CTAG
BpmI 228 CTCCAG CTGGAG(16/14)
738 CTCCAG
BsaAI 514 TACGTG [CT]AC^GT[GA]
709 CACGTG
BsaWI 33 ACCGGT [AT]^CCGG[AT]
533 ACCGGA
BsgI 71 GTGCAG GTGCAG(16/14)
692 GTGCAG
BsrDI 252 CATTGC GCAATG(2/0)
360 GCAATG
BstUI 46 CGCG CG^CG
126 CGCG
HapII 34 CCGG C^CGG
534 CCGG
HpaII 34 CCGG C^CGG
534 CCGG
MaeI 88 CTAG C^TAG
301 CTAG
MaeII 515 ACGT A^CGT
710 ACGT
MboII 148 GAAGA GAAGA(8/7)
270 GAAGA
MspI 34 CCGG C^CGG
534 CCGG
MvnI 46 CGCG CG^CG
126 CGCG
PvuII 221 CAGCTG CAG^CTG
407 CAGCTG
SfaNI 676 GATGC GCATC(5/9)
395 GCATC
TfiI 158 GAATC G^A[AT]TC
368 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsmAI BssKI BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I FauI
Fnu4HI HaeIII HhaI HinP1I HinfI
HphI ItaI MboI MflI MnlI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
TspRI TthHB8I XhoII