(Circular) (Six-base) MAPSORT of: pFN21AE0915.seq Check: 1152 from: 1 to: 5494 pFN21AE0915 5494 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3557 4941 5209 1632 Size: 1925 1384 268 1917 Fragments arranged by size: 1925 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 2038 4325 2038 Size: 2287 3207 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2350 1184 Size: 1166 4328 AlwNI CAG_nnn'CTG Cuts at: 2343 3237 4809 2343 Size: 894 1572 3028 Fragments arranged by size: 3028 1572 894 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2803 3029 3181 5290 1493 Size: 1310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2819 1870 Size: 73 876 4545 Fragments arranged by size: 4545 876 73 BamHI G'GATC_C Cuts at: 2733 2824 3191 2733 Size: 91 367 5036 Fragments arranged by size: 5036 367 91 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2309 2474 2815 4270 618 Size: 325 217 627 522 165 341 1455 1842 Fragments arranged by size: 1842 1455 627 522 341 325 217 165 BanII G_rGCy'C Cuts at: 1391 2813 1391 Size: 1422 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BfrBI ATG'CAT Cuts at: 2585 2585 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4105 136 Size: 107 122 71 631 605 2433 1525 Fragments arranged by size: 2433 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2080 5489 2080 Size: 3409 2085 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2477 3555 4711 1790 Size: 687 1078 1156 2573 Fragments arranged by size: 2573 1156 1078 687 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4183 442 Size: 416 53 83 399 24 1808 958 1753 Fragments arranged by size: 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2218 2728 4154 1239 Size: 979 510 1426 2579 Fragments arranged by size: 2579 1426 979 510 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2023 4157 882 Size: 617 524 2134 2219 Fragments arranged by size: 2219 2134 617 524 BsaAI yAC'GTr Cuts at: 493 1204 2521 2716 3285 493 Size: 711 1317 195 569 2702 Fragments arranged by size: 2702 1317 711 569 195 BsaBI GATnn'nnATC Cuts at: 2288 5488 2288 Size: 3200 2294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3301 3683 4318 275 Size: 53 83 186 936 1768 382 635 1451 Fragments arranged by size: 1768 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2038 2538 3927 4325 4599 4746 5157 2038 Size: 500 1389 398 274 147 411 2375 Fragments arranged by size: 2375 1389 500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2095 2125 2205 2235 2246 Size: 30 165 30 692 30 80 30 11 Cuts at: 2246 2276 2685 2715 1178 Size: 30 409 30 3957 Fragments arranged by size: 3957 692 409 165 80 30 30 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5494 BseYI C'CCAG_C Cuts at: 2139 2318 2490 4697 5193 2139 Size: 179 172 2207 496 2440 Fragments arranged by size: 2440 2207 496 179 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2097 2718 1163 Size: 573 361 621 3939 Fragments arranged by size: 3939 621 573 361 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3555 3640 4711 3555 Size: 85 1071 4338 Fragments arranged by size: 4338 1071 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2477 2813 3555 3640 4711 1391 Size: 399 687 336 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 399 336 85 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 2849 844 Size: 1466 539 3489 Fragments arranged by size: 3489 1466 539 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2256 2372 3989 4163 65 Size: 1183 1008 116 1617 174 1396 Fragments arranged by size: 1617 1396 1183 1008 174 116 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 4138 4325 1700 Size: 110 228 2100 187 2869 Fragments arranged by size: 2869 2100 228 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5494 BstBI TT'CG_AA Cuts at: 2386 4298 2386 Size: 1912 3582 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstXI CCAn_nnnn'nTGG Cuts at: 2023 2023 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2080 2089 2733 2824 3191 3576 3593 5045 Size: 108 9 644 91 367 385 17 1452 Cuts at: 5045 5131 5143 5489 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 644 385 367 346 108 91 86 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2004 2209 2313 2469 513 Size: 552 939 205 104 156 3538 Fragments arranged by size: 3538 939 552 205 156 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 3010 3804 3824 3010 Size: 794 20 4680 Fragments arranged by size: 4680 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2070 4501 809 Size: 507 754 2431 1802 Fragments arranged by size: 2431 1802 754 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2941 3830 Size: 54 1425 50 275 18 297 814 889 Cuts at: 3830 5459 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 814 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 3424 1353 Size: 795 1276 3423 Fragments arranged by size: 3423 1276 795 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2195 4081 4455 4601 387 Size: 1064 138 606 1886 374 146 1280 Fragments arranged by size: 1886 1280 1064 606 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2218 2728 3557 4154 4941 5209 1239 Size: 393 586 510 829 597 787 268 1524 Fragments arranged by size: 1524 829 787 597 586 510 393 268 EcoO109I rG'GnC_Cy Cuts at: 2504 2504 Size: 5494 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 2012 4641 1678 Size: 334 2629 2531 Fragments arranged by size: 2629 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2685 2704 2717 4135 Size: 32 135 32 1100 13 19 13 1418 Cuts at: 4135 4167 4209 4241 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1418 1100 135 42 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2704 2838 3084 669 Size: 988 281 766 134 246 3079 Fragments arranged by size: 3079 988 766 281 246 134 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 4608 4792 1305 Size: 699 2604 184 2007 Fragments arranged by size: 2604 2007 699 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3672 Fragments arranged by size: 3672 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3452 3811 3970 518 Size: 648 2286 359 159 2042 Fragments arranged by size: 2286 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2228 2246 2330 2414 2910 3587 4735 4980 Size: 622 18 84 84 496 677 1148 245 Cuts at: 4980 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 622 496 245 84 84 18 NcoI C'CATG_G Cuts at: 513 1065 2004 2209 2313 2469 513 Size: 552 939 205 104 156 3538 Fragments arranged by size: 3538 939 552 205 156 104 NdeI CA'TA_TG Cuts at: 387 2587 387 Size: 2200 3294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NsiI A_TGCA'T Cuts at: 2587 2587 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PmlI CAC'GTG Cuts at: 2716 2716 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PstI C_TGCA'G Cuts at: 830 2846 830 Size: 2016 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2228 2414 2228 Size: 186 5308 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SapI GCTCTTCn'nnn_ Cuts at: 2148 2148 Size: 5494 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2241 2406 2562 2674 2842 3977 4658 Size: 219 1196 165 156 112 168 1135 681 Cuts at: 4658 4849 826 Size: 191 1471 Fragments arranged by size: 1471 1196 1135 681 219 191 168 165 156 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3598 4499 4761 820 Size: 197 926 1655 901 262 1553 Fragments arranged by size: 1655 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 2636 1517 Size: 1119 4375 StyI C'CwwG_G Cuts at: 513 1065 2004 2209 2313 2469 2933 513 Size: 552 939 205 104 156 464 3074 Fragments arranged by size: 3074 939 552 464 205 156 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3740 5452 Size: 274 80 33 51 1443 471 1292 1712 Cuts at: 5452 96 Size: 138 Fragments arranged by size: 1712 1443 1292 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 5454 5486 2095 Size: 32 3327 32 2103 Fragments arranged by size: 3327 2103 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2752 2752 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 2172 3623 2172 Size: 1451 4043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI