Restriction map for
pFN21AE0907
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BseAI
BsgI BsiHKAI BsiWI BsiYI BslI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeIII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TseI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 544 GTATAC GT^[AC][GT]AC
AfaI 225 GTAC GT^AC
BanII 218 GGGCTC G[GA]GC[CT]^C
BbsI 208 GAAGAC GAAGAC(2/6)
BpuAI 208 GAAGAC GAAGAC(2/6)
BsaWI 15 ACCGGA [AT]^CCGG[AT]
BseRI 55 GAGGAG GAGGAG(10/8)
BsiEI 378 CGATCG CG[GA][CT]^CG
BsmBI 363 GAGACG CGTCTC(1/5)
BsmFI 594 GTCCC GGGAC(10/14)
Bsp1407I 224 TGTACA T^GTACA
BspMI 176 ACCTGC ACCTGC(4/8)
BsrBI 618 GAGCGG CCGCTC(-3/-3)
BsrGI 224 TGTACA T^GTACA
BsrI 237 CCAGT ACTGG(1/-1)
BssKI 259 CCTGG ^CC[ACGT]GG
Bst1107I 544 GTATAC GTA^TAC
BstNI 259 CCTGG CC^[AT]GG
BstZ17I 544 GTATAC GTA^TAC
Cac8I 168 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 452 GCGC GCG^C
DdeI 614 CTAAG C^T[ACGT]AG
DraI 142 TTTAAA TTT^AAA
EarI 52 GAAGAG CTCTTC(1/4)
EcoRII 259 CCTGG ^CC[AT]GG
HaeII 451 GGCGCT [GA]GCGC^[CT]
HapII 16 CCGG C^CGG
HhaI 452 GCGC GCG^C
HinP1I 452 GCGC G^CGC
HpaII 16 CCGG C^CGG
HphI 478 GGTGA GGTGA(8/7)
Ksp632I 52 GAAGAG CTCTTC(1/4)
MslI 398 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 16 CCGG C^CGG
MvaI 259 CCTGG CC^[AT]GG
MwoI 601 GCAGACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 622 GGCTCC GG[ACGT]^[ACGT]CC
PvuI 378 CGATCG CGAT^CG
RsaI 225 GTAC GT^AC
ScrFI 259 CCTGG CC^[ACGT]GG
SspBI 224 TGTACA T^GTACA
TspRI 435 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 180 GCGG CCGC(-3/-1)
620 GCGG
BfaI 245 CTAG C^TAG
391 CTAG
BmyI 128 GGGCAC G[AGT]GC[ACT]^C
218 GGGCTC
BsmAI 363 GAGAC GTCTC(1/5)
394 GAGAC
Bsp1286I 128 GGGCAC G[AGT]GC[ACT]^C
218 GGGCTC
BstF5I 109 CATCC GGATG(2/0)
285 GGATG
DpnII 186 GATC ^GATC
379 GATC
Fnu4HI 180 GCGGC GC^[ACGT]GC
620 GCGGC
FokI 109 CATCC GGATG(9/13)
285 GGATG
HinfI 67 GAATC G^A[ACGT]TC
418 GAATC
ItaI 180 GCGGC GC^[ACGT]GC
620 GCGGC
MaeI 245 CTAG C^TAG
391 CTAG
MboI 186 GATC ^GATC
379 GATC
NdeII 186 GATC ^GATC
379 GATC
Sau3AI 186 GATC ^GATC
379 GATC
TaqI 11 TCGA T^CGA
377 TCGA
TfiI 67 GAATC G^A[AT]TC
418 GAATC
Tsp45I 266 GTGAC ^GT[GC]AC
371 GTGAC
Tsp509I 250 AATT ^AATT
342 AATT
TthHB8I 11 TCGA T^CGA
377 TCGA
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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AluI MaeIII MboII MnlI MseI
SfcI Tru9I