(Circular) (Six-base) MAPSORT of: pFN21AE0738.seq Check: 4539 from: 1 to: 5977 pFN21AE0738 5977 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2335 2335 Size: 5977 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2479 2881 3234 4040 5424 5692 1632 Size: 847 402 353 806 1384 268 1917 Fragments arranged by size: 1917 1384 847 806 402 353 268 AfeI AGC'GCT Cuts at: 2201 2201 Size: 5977 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 2954 1166 Size: 1788 4189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2269 2281 3000 3720 5292 2269 Size: 12 719 720 1572 2954 Fragments arranged by size: 2954 1572 720 719 12 ApaI G_GGCC'C Cuts at: 2312 2312 Size: 5977 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2781 2796 3121 3302 1870 Size: 73 100 738 15 325 181 4545 Fragments arranged by size: 4545 738 325 181 100 73 15 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 3254 3298 4753 618 Size: 325 217 627 261 1206 44 1455 1842 Fragments arranged by size: 1842 1455 1206 627 325 261 217 44 BanII G_rGCy'C Cuts at: 1391 2312 2546 2890 3296 1391 Size: 921 234 344 406 4072 Fragments arranged by size: 4072 921 406 344 234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2503 928 Size: 971 604 4402 Fragments arranged by size: 4402 971 604 BbvCI CC'TCA_GC Cuts at: 3170 3176 3182 3170 Size: 6 6 5965 Fragments arranged by size: 5965 6 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2190 4588 136 Size: 107 122 71 631 605 518 2398 1525 Fragments arranged by size: 2398 1525 631 605 518 122 107 71 BglII A'GATC_T Cuts at: 2679 5972 2679 Size: 3293 2684 BlpI GC'TnA_GC Cuts at: 2351 3394 2351 Size: 1043 4934 Bme1580I G_kGCm'C Cuts at: 1790 2051 2312 4038 5194 1790 Size: 261 261 1726 1156 2573 Fragments arranged by size: 2573 1726 1156 261 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2755 3136 4637 1239 Size: 1178 338 381 1501 2579 Fragments arranged by size: 2579 1501 1178 381 338 Bpu10I CC'TnA_GC Cuts at: 2545 2767 3170 3176 3182 2545 Size: 222 403 6 6 5340 Fragments arranged by size: 5340 403 222 6 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2490 4102 4967 5265 2490 Size: 1612 865 298 3202 Fragments arranged by size: 3202 1612 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3257 4640 882 Size: 617 1758 1383 2219 Fragments arranged by size: 2219 1758 1383 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2739 1186 Size: 925 628 4424 Fragments arranged by size: 4424 925 628 BseYI C'CCAG_C Cuts at: 2083 2188 2562 2883 3081 5180 5676 2083 Size: 105 374 321 198 2099 496 2384 Fragments arranged by size: 2384 2099 496 374 321 198 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 2344 1163 Size: 573 474 134 4796 Fragments arranged by size: 4796 573 474 134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2151 3427 4188 5216 1540 Size: 275 186 150 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 150 BsiHKAI G_wGCw'C Cuts at: 2352 4038 4123 5194 2352 Size: 1686 85 1071 3135 Fragments arranged by size: 3135 1686 1071 85 BsmI GAATG_Cn' Cuts at: 2935 3488 3581 2935 Size: 553 93 5331 Fragments arranged by size: 5331 553 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2312 2352 2546 2890 3296 4038 Size: 399 261 261 40 194 344 406 742 Cuts at: 4038 4123 5194 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 344 261 261 194 85 40 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2335 2349 2466 3332 844 Size: 1491 14 117 866 3489 Fragments arranged by size: 3489 1491 866 117 14 BsrBI CCG'CTC Cuts at: 1394 1823 3150 3864 1394 Size: 429 1327 714 3507 Fragments arranged by size: 3507 1327 714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2635 3096 4621 4808 1700 Size: 110 825 461 1525 187 2869 Fragments arranged by size: 2869 1525 825 461 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5977 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 2638 1184 Size: 1454 4523 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2392 2956 2137 Size: 255 564 5158 Fragments arranged by size: 5158 564 255 BstYI r'GATC_y Cuts at: 1972 2679 2832 3012 3307 3674 4059 4076 5528 Size: 707 153 180 295 367 385 17 1452 Cuts at: 5528 5614 5626 5972 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 707 385 367 346 295 180 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2250 2591 3044 513 Size: 552 939 246 341 453 3446 Fragments arranged by size: 3446 939 552 453 341 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2449 2860 487 Size: 683 56 1223 411 3604 Fragments arranged by size: 3604 1223 683 411 56 BtsI GCAGTG_nn' Cuts at: 3493 4287 4307 3493 Size: 794 20 5163 Fragments arranged by size: 5163 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2127 2247 Size: 54 1425 50 275 18 273 24 120 Cuts at: 2247 2382 2484 3162 3424 4313 5942 8 Size: 135 102 678 262 889 1629 43 Fragments arranged by size: 1629 1425 889 678 275 273 262 135 120 102 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 3210 3907 1353 Size: 911 946 697 3423 Fragments arranged by size: 3423 946 911 697 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2479 2755 2881 3136 3234 4040 Size: 393 785 62 276 126 255 98 806 Cuts at: 4040 4637 5424 5692 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 806 787 785 597 393 276 268 255 126 98 62 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 5977 EcoO109I rG'GnC_Cy Cuts at: 2308 2309 2751 3077 2308 Size: 1 442 326 5208 Fragments arranged by size: 5208 442 326 1 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2869 2869 Size: 5977 FspI TGC'GCA Cuts at: 2426 4483 2426 Size: 2057 3920 HaeII r_GCGC'y Cuts at: 1678 2203 2258 5124 1678 Size: 525 55 2866 2531 Fragments arranged by size: 2866 2531 525 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2738 2770 4618 4650 4692 Size: 32 135 32 1166 32 1848 32 42 Cuts at: 4692 4724 1373 Size: 32 2626 Fragments arranged by size: 2626 1848 1166 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 5091 5275 1305 Size: 1827 1959 184 2007 Fragments arranged by size: 2007 1959 1827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 2129 2384 10 Size: 54 1425 343 273 24 255 3603 Fragments arranged by size: 3603 1425 343 273 255 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2372 2954 2972 3935 4294 4453 518 Size: 648 1206 582 18 963 359 159 2042 Fragments arranged by size: 2042 1206 963 648 582 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2188 2278 2997 3046 3156 3260 3393 4070 Size: 582 90 719 49 110 104 133 677 Cuts at: 4070 5218 5463 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 719 677 582 245 133 110 104 90 49 NaeI GCC'GGC Cuts at: 3098 3098 Size: 5977 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4486 Fragments arranged by size: 4486 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NgoMIV G'CCGG_C Cuts at: 3096 3096 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2212 2359 2377 3335 4880 1132 Size: 97 983 147 18 958 1545 2229 Fragments arranged by size: 2229 1545 983 958 147 97 18 PasI CC'CwG_GG Cuts at: 1348 2179 1348 Size: 831 5146 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3077 3077 Size: 5977 PshAI GACnn'nnGTC Cuts at: 1613 2855 1613 Size: 1242 4735 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 2308 2308 Size: 5977 PstI C_TGCA'G Cuts at: 830 2346 3329 830 Size: 1516 983 3478 Fragments arranged by size: 3478 1516 983 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2188 2278 2997 3156 2188 Size: 90 719 159 5009 Fragments arranged by size: 5009 719 159 90 SacII CC_GC'GG Cuts at: 3047 3047 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 3077 3077 Size: 5977 SapI GCTCTTCn'nnn_ Cuts at: 2264 2264 Size: 5977 SbfI CC_TGCA'GG Cuts at: 2346 3329 2346 Size: 983 4994 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2342 2659 3325 4460 5141 5332 826 Size: 219 1297 317 666 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 681 666 317 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2190 2190 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 3096 1700 Size: 1396 4581 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2505 3121 4081 4982 5244 820 Size: 197 926 562 616 960 901 262 1553 Fragments arranged by size: 1553 960 926 901 616 562 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 2212 3335 1132 Size: 1080 1123 3774 Fragments arranged by size: 3774 1123 1080 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 2711 3211 1517 Size: 1194 500 4283 Fragments arranged by size: 4283 1194 500 StyI C'CwwG_G Cuts at: 513 1065 2004 2106 2582 3224 3416 513 Size: 552 939 102 476 642 192 3074 Fragments arranged by size: 3074 939 642 552 476 192 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2280 2280 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 3121 1943 Size: 1178 4799 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SexAI SfoI SrfI SwaI