Restriction map for
pFN21AB6634
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NcoI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AfaI 264 GTAC GT^AC
AflIII 176 ACACGT A^C[GA][CT]GT
ApaI 15 GGGCCC GGGCC^C
BamHI 629 GGATCC G^GATCC
BbeI 445 GGCGCC GGCGC^C
BpmI 450 CTGGAG CTGGAG(16/14)
BseRI 1164 CTCCTC GAGGAG(10/8)
BsmAI 703 GAGAC GTCTC(1/5)
BsmBI 703 GAGACG CGTCTC(1/5)
BsrBI 461 CCGCTC CCGCTC(-3/-3)
BssHII 283 GCGCGC G^CGCGC
BssSI 499 CACGAG CACGAG(-5/-1)
BstXI 677 CCACCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 629 GGATCC [GA]^GATC[CT]
DsaI 1274 CCGCGG C^C[GA][CT]GG
KasI 445 GGCGCC G^GCGCC
KspI 1274 CCGCGG CCGC^GG
MflI 629 GGATCC [GA]^GATC[CT]
MslI 678 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 243 GCCGGC GCC^GGC
NarI 445 GGCGCC GG^CGCC
NdeI 609 CATATG CA^TATG
NgoMIV 243 GCCGGC G^CCGGC
NheI 636 GCTAGC G^CTAGC
NspI 824 GCATGT [GA]CATG^[CT]
PvuII 1189 CAGCTG CAG^CTG
RsaI 264 GTAC GT^AC
SacII 1274 CCGCGG CCGC^GG
SapI 201 GCTCTTC GCTCTTC(1/4)
SexAI 838 ACCTGGT A^CC[AT]GGT
SfoI 445 GGCGCC GGC^GCC
TfiI 1135 GATTC G^A[AT]TC
Tsp45I 835 GTGAC ^GT[GC]AC
Tsp509I 1255 AATT ^AATT
XhoII 629 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 630 GATCC GGATC(4/5)
629 GGATC
BanII 15 GGGCCC G[GA]GC[CT]^C
1279 GAGCCC
BsgI 153 CTGCAC GTGCAG(16/14)
658 GTGCAG
BsiEI 89 CGGCCG CG[GA][CT]^CG
1291 CGGCCG
BsrFI 243 GCCGGC [GA]^CCGG[CT]
679 ACCGGC
BsrI 534 CCAGT ACTGG(1/-1)
689 CCAGT
BstAPI 651 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1101 GCAGGTGCTGC
Cfr10I 243 GCCGGC [GA]^CCGG[CT]
679 ACCGGC
DpnII 141 GATC ^GATC
630 GATC
EagI 89 CGGCCG C^GGCCG
1291 CGGCCG
EclXI 89 CGGCCG C^GGCCG
1291 CGGCCG
Eco52I 89 CGGCCG C^GGCCG
1291 CGGCCG
EcoNI 620 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1095 CCTACAGCAGG
MboI 141 GATC ^GATC
630 GATC
NdeII 141 GATC ^GATC
630 GATC
NotI 88 GCGGCCGC GC^GGCCGC
1290 GCGGCCGC
PpuMI 721 AGGACCC [GA]G^G[AT]CC[CT]
1217 AGGACCC
Sau3AI 141 GATC ^GATC
630 GATC
SmaI 66 CCCGGG CCC^GGG
1022 CCCGGG
StuI 695 AGGCCT AGG^CCT
897 AGGCCT
TspRI 535 CAGTG CA[GC]TG[ACGT][ACGT]^
1055 CACTG
XcmI 182 CCAACTTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
797 CCAGCCTGACGCTGG
XmaCI 66 CCCGGG C^CCGGG
1022 CCCGGG
XmaI 66 CCCGGG C^CCGGG
1022 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII AviII BalI BanI
BbvI BcnI BfaI BglI BmyI
BsaHI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI PstI Sau96I
ScrFI SfaNI SfcI SfiI TaqI
TseI TthHB8I