Restriction map for
pFN21AB6634
(Circular) (Six-base) MAPSORT of: pFN21AB6634.seq Check: 8954 from: 1 to: 6025
pFN21AB6634 6025 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2656 2707 3097 2656
Size: 51 390 5584
Fragments arranged by size:
5584 390 51
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 3324 4152 4525 1563
Size: 1761 828 373 3063
Fragments arranged by size:
3063 1761 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 4088 5472 5740 1632
Size: 1184 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 1184 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 2181 4924 5901 1203
Size: 978 2743 977 1327
Fragments arranged by size:
2743 1327 978 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2654 3023 1184
Size: 1470 369 4186
Fragments arranged by size:
4186 1470 369
AlwNI CAG_nnn'CTG
Cuts at: 2085 3112 3199 3768 5340 2085
Size: 1027 87 569 1572 2770
Fragments arranged by size:
2770 1572 1027 569 87
ApaI G_GGCC'C
Cuts at: 2024 2024
Size: 6025
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 3334 3560 3712 5821 1493
Size: 1841 226 152 2109 1697
Fragments arranged by size:
2109 1841 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2605 3027 3172 3287 3350 1870
Size: 73 128 534 422 145 115 63 4545
Fragments arranged by size:
4545 534 422 145 128 115 73 63
BamHI G'GATC_C
Cuts at: 2634 3355 3722 2634
Size: 721 367 4937
Fragments arranged by size:
4937 721 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 2609 2719 3346 4801
Size: 325 217 627 663 159 110 627 1455
Cuts at: 4801 618
Size: 1842
Fragments arranged by size:
1842 1455 663 627 627 325 217 159
110
BanII G_rGCy'C
Cuts at: 1391 2024 3288 3344 1391
Size: 633 1264 56 4072
Fragments arranged by size:
4072 1264 633 56
BbeI G_GCGC'C
Cuts at: 2454 2454
Size: 6025
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2699 2798
Size: 107 122 71 631 605 379 648 99
Cuts at: 2798 3047 4636 136
Size: 249 1589 1525
Fragments arranged by size:
1589 1525 648 631 605 379 249 122
107 99 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2434 2724 4086 5242 1790
Size: 234 410 290 1362 1156 2573
Fragments arranged by size:
2573 1362 1156 410 290 234
BmgBI CAC'GTC
Cuts at: 1318 2184 2214 2323 1318
Size: 866 30 109 5020
Fragments arranged by size:
5020 866 109 30
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2645 1056
Size: 1589 4436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 4685 1239
Size: 1237 2209 2579
Fragments arranged by size:
2579 2209 1237
Bpu10I CC'TnA_GC
Cuts at: 3095 3160 3095
Size: 65 5960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4688 882
Size: 617 3189 2219
Fragments arranged by size:
3189 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2420 2451 2579 2764
Size: 53 83 186 936 887 31 128 185
Cuts at: 2764 3832 4214 4849 275
Size: 1068 382 635 1451
Fragments arranged by size:
1451 1068 936 887 635 382 186 185
128 83 53 31
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2879 2909 1178
Size: 30 165 30 1476 30 4294
Fragments arranged by size:
4294 1476 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3160 1186
Size: 1974 4051
BseYI C'CCAG_C
Cuts at: 2085 2277 2494 2509 2812 2824 2926 2995 3192
Size: 192 217 15 303 12 102 69 197
Cuts at: 3192 5228 5724 2085
Size: 2036 496 2386
Fragments arranged by size:
2386 2036 496 303 217 197 192 102
69 15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2143 2684 1163
Size: 573 407 541 4504
Fragments arranged by size:
4504 573 541 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 3299 3475 4236 5264 1540
Size: 275 186 96 1202 176 761 1028 2301
Fragments arranged by size:
2301 1202 1028 761 275 186 176 96
BsiHKAI G_wGCw'C
Cuts at: 4086 4171 5242 4086
Size: 85 1071 4869
Fragments arranged by size:
4869 1071 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
BsmBI CGTCTCn'nnnn_
Cuts at: 2702 2702
Size: 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2434 2724 3288 3344 4086 4171
Size: 399 234 410 290 564 56 742 85
Cuts at: 4171 5242 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 564 410 399 290 234
85 56
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2656 2707 2991 3097 3380 844
Size: 1544 268 51 284 106 283 3489
Fragments arranged by size:
3489 1544 284 283 268 106 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2468 3912 1394
Size: 429 645 1444 3507
Fragments arranged by size:
3507 1444 645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4520 4694 65
Size: 1183 3272 174 1396
Fragments arranged by size:
3272 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2684 4669 4856 1700
Size: 110 438 436 1985 187 2869
Fragments arranged by size:
2869 1985 438 436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 2288 1925
Size: 363 5662
BssSI C'ACGA_G
Cuts at: 2504 4085 5097 2504
Size: 1581 1012 3432
Fragments arranged by size:
3432 1581 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2662 3112 794
Size: 1868 450 3707
Fragments arranged by size:
3707 1868 450
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2689
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2634 3355 3722 4107 4124 5576 5662 5674
Size: 662 721 367 385 17 1452 86 12
Cuts at: 5674 6020 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 721 662 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3279 513
Size: 552 939 1275 3259
Fragments arranged by size:
3259 1275 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2098 487
Size: 683 56 872 4414
Fragments arranged by size:
4414 872 683 56
BtsI GCAGTG_nn'
Cuts at: 3059 3541 4335 4355 3059
Size: 482 794 20 4729
Fragments arranged by size:
4729 794 482 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2130 2250
Size: 54 1425 50 275 18 264 36 120
Cuts at: 2250 2522 2536 2553 2691 2862 3296 3472 4361
Size: 272 14 17 138 171 434 176 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 434 275 272 264 176
171 138 120 54 50 43 36 18
17 14
EagI C'GGCC_G
Cuts at: 1537 1812 2094 3296 3472 1537
Size: 275 282 1202 176 4090
Fragments arranged by size:
4090 1202 282 275 176
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2912 2975 3955 1353
Size: 860 699 63 980 3423
Fragments arranged by size:
3423 980 860 699 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2476 2816 4088 4685 5472 5740 1239
Size: 393 844 340 1272 597 787 268 1524
Fragments arranged by size:
1524 1272 844 787 597 393 340 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2629 3104 2629
Size: 475 5550
EcoO109I rG'GnC_Cy
Cuts at: 2727 2791 3223 2727
Size: 64 432 5529
Fragments arranged by size:
5529 432 64
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 2177 2414 3065 4531 2177
Size: 237 651 1466 3671
Fragments arranged by size:
3671 1466 651 237
HaeII r_GCGC'y
Cuts at: 1678 2278 2340 2454 2996 3037 5172 1678
Size: 600 62 114 542 41 2135 2531
Fragments arranged by size:
2531 2135 600 542 114 62 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2879 2911 3131 3143 3163
Size: 32 135 32 1307 32 220 12 20
Cuts at: 3163 3175 4666 4698 4740 4772 1373
Size: 12 1491 32 42 32 2626
Fragments arranged by size:
2626 1491 1307 220 135 42 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KasI G'GCGC_C
Cuts at: 2450 2450
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 5139 5323 1305
Size: 906 2928 184 2007
Fragments arranged by size:
2928 2007 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2538 2555 2693 2864 10
Size: 54 1425 343 706 17 138 171 3171
Fragments arranged by size:
3171 1425 706 343 171 138 54 17
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 3983 4342 4501 518
Size: 648 1521 1296 359 159 2042
Fragments arranged by size:
2042 1521 1296 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2398 3078 3118 3196 3232 3248 3281
Size: 476 316 680 40 78 36 16 33
Cuts at: 3281 3441 4118 5266 5511 1606
Size: 160 677 1148 245 2120
Fragments arranged by size:
2120 1148 680 677 476 316 245 160
78 40 36 33 16
NaeI GCC'GGC
Cuts at: 2250 2250
Size: 6025
NarI GG'CG_CC
Cuts at: 2451 2451
Size: 6025
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 2615 387
Size: 2228 3797
NgoMIV G'CCGG_C
Cuts at: 2248 2248
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 2641 1052
Size: 1589 4436
NotI GC'GGCC_GC
Cuts at: 2094 3296 3472 2094
Size: 1202 176 4647
Fragments arranged by size:
4647 1202 176
NspI r_CATG'y
Cuts at: 1132 1229 2833 3383 4928 1132
Size: 97 1604 550 1545 2229
Fragments arranged by size:
2229 1604 1545 550 97
PasI CC'CwG_GG
Cuts at: 1348 2738 1348
Size: 1390 4635
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6025
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2727 3223 2727
Size: 496 5529
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PspOMI G'GGCC_C
Cuts at: 2020 2020
Size: 6025
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2252 2284 2252
Size: 32 5993
PstI C_TGCA'G
Cuts at: 830 2336 2633 2718 3377 830
Size: 1506 297 85 659 3478
Fragments arranged by size:
3478 1506 659 297 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 3196 3196
Size: 6025
SacII CC_GC'GG
Cuts at: 3282 3282
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SapI GCTCTTCn'nnn_
Cuts at: 2213 2213
Size: 6025
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SexAI A'CCwGG_T
Cuts at: 2843 2843
Size: 6025
SfcI C'TryA_G
Cuts at: 826 1045 2332 2629 2714 3101 3373 4508 5189
Size: 219 1287 297 85 387 272 1135 681
Cuts at: 5189 5380 826
Size: 191 1471
Fragments arranged by size:
1471 1287 1135 681 387 297 272 219
191 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 2699 2798 3047 2699
Size: 99 249 5677
Fragments arranged by size:
5677 249 99
SfoI GGC'GCC
Cuts at: 2452 2452
Size: 6025
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 2073 3029 3352 1872
Size: 201 956 323 4545
Fragments arranged by size:
4545 956 323 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2702 2904 1517
Size: 1185 202 4638
Fragments arranged by size:
4638 1185 202
StyI C'CwwG_G
Cuts at: 513 1065 2004 3464 513
Size: 552 939 1460 3074
Fragments arranged by size:
3074 1460 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2077 2269 3233 4052 4069 4222 4407
Size: 1255 497 192 964 819 17 153 185
Cuts at: 4407 4852 325
Size: 445 1498
Fragments arranged by size:
1498 1255 964 819 497 445 192 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3297 3329 5985 6017 3297
Size: 32 2656 32 3305
Fragments arranged by size:
3305 2656 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2809 2194
Size: 615 5410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 2071 3027 3350 1870
Size: 201 956 323 4545
Fragments arranged by size:
4545 956 323 201
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI RsrII
SacI SanDI SrfI SwaI