Restriction map for
pFN21AB6634
(Linear) (Six-base) MAPSORT of: KIBB6634.seq Check: 1693 from: 1 to: 1320
KIBB6634.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 651 702 1092 1320
Size: 651 51 390 228
Fragments arranged by size:
651 390 228 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1320
Size: 811 509
AflIII A'CryG_T
Cuts at: 0 176 1320
Size: 176 1144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 1018 1320
Size: 649 369 302
Fragments arranged by size:
649 369 302
AlwNI CAG_nnn'CTG
Cuts at: 0 80 1107 1194 1320
Size: 80 1027 87 126
Fragments arranged by size:
1027 126 87 80
ApaI G_GGCC'C
Cuts at: 0 19 1320
Size: 19 1301
AvaI C'yCGr_G
Cuts at: 0 66 600 1022 1167 1282 1320
Size: 66 534 422 145 115 38
Fragments arranged by size:
534 422 145 115 66 38
BamHI G'GATC_C
Cuts at: 0 629 1320
Size: 629 691
BanI G'GyrC_C
Cuts at: 0 445 604 714 1320
Size: 445 159 110 606
Fragments arranged by size:
606 445 159 110
BanII G_rGCy'C
Cuts at: 0 19 1283 1320
Size: 19 1264 37
Fragments arranged by size:
1264 37 19
BbeI G_GCGC'C
Cuts at: 0 449 1320
Size: 449 871
BglI GCCn_nnn'nGGC
Cuts at: 0 46 694 793 1042 1320
Size: 46 648 99 249 278
Fragments arranged by size:
648 278 249 99 46
Bme1580I G_kGCm'C
Cuts at: 0 19 429 719 1320
Size: 19 410 290 601
Fragments arranged by size:
601 410 290 19
BmgBI CAC'GTC
Cuts at: 0 179 209 318 1320
Size: 179 30 109 1002
Fragments arranged by size:
1002 179 109 30
BmtI G_CTAG'C
Cuts at: 0 640 1320
Size: 640 680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1320
Size: 471 849
Bpu10I CC'TnA_GC
Cuts at: 0 1090 1155 1320
Size: 1090 65 165
Fragments arranged by size:
1090 165 65
BsaHI Gr'CG_yC
Cuts at: 0 415 446 574 759 1320
Size: 415 31 128 185 561
Fragments arranged by size:
561 415 185 128 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 874 904 1320
Size: 874 30 416
Fragments arranged by size:
874 416 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1155 1320
Size: 1155 165
BseYI C'CCAG_C
Cuts at: 0 80 272 489 504 807 819 921 990
Size: 80 192 217 15 303 12 102 69
Cuts at: 990 1187 1320
Size: 197 133
Fragments arranged by size:
303 217 197 192 133 102 80 69
15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 679 1320
Size: 138 541 641
Fragments arranged by size:
641 541 138
BsiEI CG_ry'CG
Cuts at: 0 92 1294 1320
Size: 92 1202 26
Fragments arranged by size:
1202 92 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 697 1320
Size: 697 623
Bsp1286I G_dGCh'C
Cuts at: 0 19 429 719 1283 1320
Size: 19 410 290 564 37
Fragments arranged by size:
564 410 290 37 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 651 702 986 1092 1320
Size: 383 268 51 284 106 228
Fragments arranged by size:
383 284 268 228 106 51
BsrBI CCG'CTC
Cuts at: 0 463 1320
Size: 463 857
BsrFI r'CCGG_y
Cuts at: 0 243 679 1320
Size: 243 436 641
Fragments arranged by size:
641 436 243
BssHII G'CGCG_C
Cuts at: 0 283 1320
Size: 283 1037
BssSI C'ACGA_G
Cuts at: 0 499 1320
Size: 499 821
BstAPI GCAn_nnn'nTGC
Cuts at: 0 657 1107 1320
Size: 657 450 213
Fragments arranged by size:
657 450 213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1320
Size: 684 636
BstYI r'GATC_y
Cuts at: 0 629 1320
Size: 629 691
BtgI C'CryG_G
Cuts at: 0 1274 1320
Size: 1274 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 93 1320
Size: 93 1227
BtsI GCAGTG_nn'
Cuts at: 0 1054 1320
Size: 1054 266
EaeI y'GGCC_r
Cuts at: 0 89 125 245 517 531 548 686 857
Size: 89 36 120 272 14 17 138 171
Cuts at: 857 1291 1320
Size: 434 29
Fragments arranged by size:
434 272 171 138 120 89 36 29
17 14
EagI C'GGCC_G
Cuts at: 0 89 1291 1320
Size: 89 1202 29
Fragments arranged by size:
1202 89 29
EarI CTCTTCn'nnn_
Cuts at: 0 208 907 970 1320
Size: 208 699 63 350
Fragments arranged by size:
699 350 208 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 811 1320
Size: 471 340 509
Fragments arranged by size:
509 471 340
EcoNI CCTnn'n_nnAGG
Cuts at: 0 624 1099 1320
Size: 624 475 221
Fragments arranged by size:
624 475 221
EcoO109I rG'GnC_Cy
Cuts at: 0 722 786 1218 1320
Size: 722 64 432 102
Fragments arranged by size:
722 432 102 64
FspI TGC'GCA
Cuts at: 0 172 409 1060 1320
Size: 172 237 651 260
Fragments arranged by size:
651 260 237 172
HaeII r_GCGC'y
Cuts at: 0 273 335 449 991 1032 1320
Size: 273 62 114 542 41 288
Fragments arranged by size:
542 288 273 114 62 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 1126 1138 1158 1170 1320
Size: 874 32 220 12 20 12 150
Fragments arranged by size:
874 220 150 32 20 12 12
KasI G'GCGC_C
Cuts at: 0 445 1320
Size: 445 875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1320
Size: 206 1114
MscI TGG'CCA
Cuts at: 0 533 550 688 859 1320
Size: 533 17 138 171 461
Fragments arranged by size:
533 461 171 138 17
MslI CAynn'nnrTG
Cuts at: 0 682 1320
Size: 682 638
MspA1I CmG'CkG
Cuts at: 0 77 393 1073 1113 1191 1227 1243 1276
Size: 77 316 680 40 78 36 16 33
Cuts at: 1276 1320
Size: 44
Fragments arranged by size:
680 316 78 77 44 40 36 33
16
NaeI GCC'GGC
Cuts at: 0 245 1320
Size: 245 1075
NarI GG'CG_CC
Cuts at: 0 446 1320
Size: 446 874
NdeI CA'TA_TG
Cuts at: 0 610 1320
Size: 610 710
NgoMIV G'CCGG_C
Cuts at: 0 243 1320
Size: 243 1077
NheI G'CTAG_C
Cuts at: 0 636 1320
Size: 636 684
NotI GC'GGCC_GC
Cuts at: 0 89 1291 1320
Size: 89 1202 29
Fragments arranged by size:
1202 89 29
NspI r_CATG'y
Cuts at: 0 828 1320
Size: 828 492
PasI CC'CwG_GG
Cuts at: 0 733 1320
Size: 733 587
PpuMI rG'GwC_Cy
Cuts at: 0 722 1218 1320
Size: 722 496 102
Fragments arranged by size:
722 496 102
PspOMI G'GGCC_C
Cuts at: 0 15 1320
Size: 15 1305
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 247 279 1320
Size: 247 32 1041
Fragments arranged by size:
1041 247 32
PstI C_TGCA'G
Cuts at: 0 331 628 713 1320
Size: 331 297 85 607
Fragments arranged by size:
607 331 297 85
PvuII CAG'CTG
Cuts at: 0 1191 1320
Size: 1191 129
SacII CC_GC'GG
Cuts at: 0 1277 1320
Size: 1277 43
SapI GCTCTTCn'nnn_
Cuts at: 0 208 1320
Size: 208 1112
SexAI A'CCwGG_T
Cuts at: 0 838 1320
Size: 838 482
SfcI C'TryA_G
Cuts at: 0 327 624 709 1096 1320
Size: 327 297 85 387 224
Fragments arranged by size:
387 327 297 224 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 694 793 1042 1320
Size: 694 99 249 278
Fragments arranged by size:
694 278 249 99
SfoI GGC'GCC
Cuts at: 0 447 1320
Size: 447 873
SmaI CCC'GGG
Cuts at: 0 68 1024 1320
Size: 68 956 296
Fragments arranged by size:
956 296 68
StuI AGG'CCT
Cuts at: 0 697 899 1320
Size: 697 202 421
Fragments arranged by size:
697 421 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 72 264 1228 1320
Size: 72 192 964 92
Fragments arranged by size:
964 192 92 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1292 1320
Size: 1292 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 804 1320
Size: 189 615 516
Fragments arranged by size:
615 516 189
XmaI C'CCGG_G
Cuts at: 0 66 1022 1320
Size: 66 956 298
Fragments arranged by size:
956 298 66
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BglI Bme1580I BmgBI BmtI BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI EaeI EagI EarI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NspI PasI PpuMI PspOMI PsrI PstI PvuII SacII
SapI SexAI SfcI SfiI SfoI SmaI StuI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaWI
BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NcoI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacI SalI SanDI SbfI
ScaI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI