(Circular) (Six-base) MAPSORT of: pFN21AB6715.seq Check: 8003 from: 1 to: 4948 pFN21AB6715 4948 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2758 278 Size: 53 83 186 936 1222 2468 Fragments arranged by size: 2468 1222 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2291 1937 Size: 354 4594 Acc65I G'GTAC_C Cuts at: 2269 2269 Size: 4948 AclI AA'CG_TT Cuts at: 1563 3075 3448 1563 Size: 1512 373 3063 Fragments arranged by size: 3063 1512 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 4395 4663 1632 Size: 1379 1384 268 1917 Fragments arranged by size: 1917 1384 1379 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4751 AflIII A'CryG_T Cuts at: 1203 3847 4824 1203 Size: 2644 977 1327 Fragments arranged by size: 2644 1327 977 AgeI A'CCGG_T Cuts at: 3779 3779 Size: 4948 AhdI GACnn_n'nnGTC Cuts at: 3677 3677 Size: 4948 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4948 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4948 AlwNI CAG_nnn'CTG Cuts at: 2174 2691 4263 2174 Size: 517 1572 2859 Fragments arranged by size: 2859 1572 517 ApaLI G'TGCA_C Cuts at: 3005 4161 3005 Size: 1156 3792 ApoI r'AATT_y Cuts at: 1493 2257 2483 2635 4744 1493 Size: 764 226 152 2109 1697 Fragments arranged by size: 2109 1697 764 226 152 AseI AT'TA_AT Cuts at: 160 1032 3502 160 Size: 872 2470 1606 Fragments arranged by size: 2470 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4948 AvaI C'yCGr_G Cuts at: 1870 1943 2273 1870 Size: 73 330 4545 Fragments arranged by size: 4545 330 73 BamHI G'GATC_C Cuts at: 2278 2645 2278 Size: 367 4581 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2269 3724 618 Size: 325 217 627 482 1455 1842 Fragments arranged by size: 1842 1455 627 482 325 217 BanII G_rGCy'C Cuts at: 1391 2267 1391 Size: 876 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2027 2171 928 Size: 971 128 144 3705 Fragments arranged by size: 3705 971 144 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3139 3173 1903 Size: 34 50 34 1118 34 3678 Fragments arranged by size: 3678 1118 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2840 4050 2840 Size: 1210 3738 BclI T'GATC_A Cuts at: 1548 1548 Size: 4948 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3559 136 Size: 107 122 71 631 605 1887 1525 Fragments arranged by size: 1887 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4943 4943 Size: 4948 BlpI GC'TnA_GC Cuts at: 2365 2365 Size: 4948 Bme1580I G_kGCm'C Cuts at: 1790 3009 4165 1790 Size: 1219 1156 2573 Fragments arranged by size: 2573 1219 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2679 3637 442 Size: 416 53 83 399 24 1262 958 1753 Fragments arranged by size: 1753 1262 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4948 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3608 1239 Size: 2369 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3073 3938 4236 3073 Size: 865 298 3785 Fragments arranged by size: 3785 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3611 882 Size: 617 2112 2219 Fragments arranged by size: 2219 2112 617 BsaAI yAC'GTr Cuts at: 493 1204 2739 493 Size: 711 1535 2702 Fragments arranged by size: 2702 1535 711 BsaBI GATnn'nnATC Cuts at: 4942 4942 Size: 4948 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2755 3137 3772 275 Size: 53 83 186 936 1222 382 635 1451 Fragments arranged by size: 1451 1222 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2030 3381 3779 4053 4200 4611 2030 Size: 1351 398 274 147 411 2367 Fragments arranged by size: 2367 1351 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4723 Fragments arranged by size: 4723 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2241 2244 1186 Size: 925 130 3 3890 Fragments arranged by size: 3890 925 130 3 BseYI C'CCAG_C Cuts at: 4151 4647 4151 Size: 496 4452 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2398 3159 4187 1540 Size: 275 186 397 761 1028 2301 Fragments arranged by size: 2301 1028 761 397 275 186 BsiHKAI G_wGCw'C Cuts at: 3009 3094 4165 3009 Size: 85 1071 3792 Fragments arranged by size: 3792 1071 85 BsmI GAATG_Cn' Cuts at: 2459 2552 2459 Size: 93 4855 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2267 3009 3094 4165 1391 Size: 399 477 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 477 399 85 BspEI T'CCGG_A Cuts at: 4611 4611 Size: 4948 BspHI T'CATG_A Cuts at: 2837 4567 2837 Size: 1730 3218 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 2303 844 Size: 1392 67 3489 Fragments arranged by size: 3489 1392 67 BsrBI CCG'CTC Cuts at: 1394 1823 2835 1394 Size: 429 1012 3507 Fragments arranged by size: 3507 1012 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3443 3617 65 Size: 1183 2195 174 1396 Fragments arranged by size: 2195 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3592 3779 1700 Size: 110 1782 187 2869 Fragments arranged by size: 2869 1782 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4948 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4948 BssSI C'ACGA_G Cuts at: 3008 4020 3008 Size: 1012 3936 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4948 BstBI TT'CG_AA Cuts at: 3752 3752 Size: 4948 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4948 BstYI r'GATC_y Cuts at: 1972 2278 2645 3030 3047 4499 4585 4597 4943 Size: 306 367 385 17 1452 86 12 346 Cuts at: 4943 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 306 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4396 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4209 Fragments arranged by size: 4209 683 56 BtsI GCAGTG_nn' Cuts at: 2464 3258 3278 2464 Size: 794 20 4134 Fragments arranged by size: 4134 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4913 DraI TTT'AAA Cuts at: 2252 2252 Size: 4948 DraIII CAC_nnn'GTG Cuts at: 2212 2212 Size: 4948 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3955 809 Size: 507 2639 1802 Fragments arranged by size: 2639 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2395 3284 4913 Size: 54 1425 50 275 18 565 889 1629 Cuts at: 4913 8 Size: 43 Fragments arranged by size: 1629 1425 889 565 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2395 1537 Size: 275 583 4090 Fragments arranged by size: 4090 583 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2878 1353 Size: 1525 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3535 3909 4055 387 Size: 1064 138 1946 374 146 1280 Fragments arranged by size: 1946 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3011 3608 4395 4663 1239 Size: 393 1379 597 787 268 1524 Fragments arranged by size: 1524 1379 787 597 393 268 EcoRI G'AATT_C Cuts at: 2257 2635 2257 Size: 378 4570 FspI TGC'GCA Cuts at: 3454 3454 Size: 4948 HaeII r_GCGC'y Cuts at: 1678 4095 1678 Size: 2417 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3589 3621 3663 3695 1373 Size: 32 135 32 2017 32 42 32 2626 Fragments arranged by size: 2626 2017 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2292 2538 669 Size: 988 281 354 246 3079 Fragments arranged by size: 3079 988 354 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4948 HpaI GTT'AAC Cuts at: 2538 2538 Size: 4948 KpnI G_GTAC'C Cuts at: 2273 2273 Size: 4948 MfeI C'AATT_G Cuts at: 2547 2547 Size: 4948 MluI A'CGCG_T Cuts at: 4824 4824 Size: 4948 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4062 4246 1305 Size: 2757 184 2007 Fragments arranged by size: 2757 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3126 Fragments arranged by size: 3126 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2906 3265 3424 518 Size: 648 1740 359 159 2042 Fragments arranged by size: 2042 1740 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2174 2364 3041 4189 4434 1606 Size: 568 190 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 568 245 190 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4396 NdeI CA'TA_TG Cuts at: 387 387 Size: 4948 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4948 NotI GC'GGCC_GC Cuts at: 2395 2395 Size: 4948 NspI r_CATG'y Cuts at: 1132 1229 2306 3851 1132 Size: 97 1077 1545 2229 Fragments arranged by size: 2229 1545 1077 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4948 PciI A'CATG_T Cuts at: 3847 3847 Size: 4948 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4948 PmeI GTTT'AAAC Cuts at: 2252 2252 Size: 4948 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3017 3049 4530 4562 3017 Size: 32 1481 32 3403 Fragments arranged by size: 3403 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4948 PsiI TTA'TAA Cuts at: 2518 2518 Size: 4948 PstI C_TGCA'G Cuts at: 830 2300 830 Size: 1470 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4948 PvuII CAG'CTG Cuts at: 2174 2174 Size: 4948 SalI G'TCGA_C Cuts at: 1936 2290 1936 Size: 354 4594 SbfI CC_TGCA'GG Cuts at: 2300 2300 Size: 4948 ScaI AGT'ACT Cuts at: 3196 4908 3196 Size: 1712 3236 SexAI A'CCwGG_T Cuts at: 2208 2208 Size: 4948 SfcI C'TryA_G Cuts at: 826 1045 2061 2137 2296 3431 4112 4303 826 Size: 219 1016 76 159 1135 681 191 1471 Fragments arranged by size: 1471 1135 1016 681 219 191 159 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4948 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4948 SmaI CCC'GGG Cuts at: 1872 2275 1872 Size: 403 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3052 3953 4215 820 Size: 197 926 1109 901 262 1553 Fragments arranged by size: 1553 1109 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2739 493 Size: 2246 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4948 SphI G_CATG'C Cuts at: 1132 2306 1132 Size: 1174 3774 SspI AAT'ATT Cuts at: 5 52 2872 5 Size: 47 2820 2081 Fragments arranged by size: 2820 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4948 StyI C'CwwG_G Cuts at: 513 1065 2387 513 Size: 552 1322 3074 Fragments arranged by size: 3074 1322 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2975 2992 3145 3330 3775 325 Size: 1255 1395 17 153 185 445 1498 Fragments arranged by size: 1498 1395 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3194 4906 96 Size: 274 80 33 51 1443 1217 1712 138 Fragments arranged by size: 1712 1443 1217 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4908 4940 4908 Size: 32 4916 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4948 XbaI T'CTAG_A Cuts at: 2284 2284 Size: 4948 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4948 XmaI C'CCGG_G Cuts at: 1870 2273 1870 Size: 403 4545 XmnI GAAnn'nnTTC Cuts at: 3077 3077 Size: 4948 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2756 276 Size: 53 83 186 936 1222 2468 Fragments arranged by size: 2468 1222 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI