Restriction map for pFN21AB6715
 (Circular) (Six-base) MAPSORT of: pFN21AB6715.seq  Check: 8003  from: 1  to: 4948



pFN21AB6715  4948 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:37  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2758     278 
   Size:         53      83     186     936    1222    2468

  Fragments arranged by size: 

                2468    1222     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2291    1937 
   Size:        354    4594
 
Acc65I G'GTAC_C

Cuts at:   2269    2269 
   Size:       4948
 
AclI AA'CG_TT

Cuts at:   1563    3075    3448    1563 
   Size:       1512     373    3063

  Fragments arranged by size: 

                3063    1512     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3011    4395    4663    1632 
   Size:       1379    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1379     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4751
 
AflIII A'CryG_T

Cuts at:   1203    3847    4824    1203 
   Size:       2644     977    1327

  Fragments arranged by size: 

                2644    1327     977
 
AgeI A'CCGG_T

Cuts at:   3779    3779 
   Size:       4948
 
AhdI GACnn_n'nnGTC

Cuts at:   3677    3677 
   Size:       4948
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       4948
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       4948
 
AlwNI CAG_nnn'CTG

Cuts at:   2174    2691    4263    2174 
   Size:        517    1572    2859

  Fragments arranged by size: 

                2859    1572     517
 
ApaLI G'TGCA_C

Cuts at:   3005    4161    3005 
   Size:       1156    3792
 
ApoI r'AATT_y

Cuts at:   1493    2257    2483    2635    4744    1493 
   Size:        764     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     764     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3502     160 
   Size:        872    2470    1606

  Fragments arranged by size: 

                2470    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4948
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2273    1870 
   Size:         73     330    4545

  Fragments arranged by size: 

                4545     330      73
 
BamHI G'GATC_C

Cuts at:   2278    2645    2278 
   Size:        367    4581
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2269    3724     618 
   Size:        325     217     627     482    1455    1842

  Fragments arranged by size: 

                1842    1455     627     482     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2267    1391 
   Size:        876    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2027    2171     928 
   Size:        971     128     144    3705

  Fragments arranged by size: 

                3705     971     144     128
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3139    3173    1903 
   Size:         34      50      34    1118      34    3678

  Fragments arranged by size: 

                3678    1118      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2840    4050    2840 
   Size:       1210    3738
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4948
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3559     136 
   Size:        107     122      71     631     605    1887    1525

  Fragments arranged by size: 

                1887    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4943    4943 
   Size:       4948
 
BlpI GC'TnA_GC

Cuts at:   2365    2365 
   Size:       4948
 
Bme1580I G_kGCm'C

Cuts at:   1790    3009    4165    1790 
   Size:       1219    1156    2573

  Fragments arranged by size: 

                2573    1219    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       4948
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2679    3637     442 
   Size:        416      53      83     399      24    1262     958    1753

  Fragments arranged by size: 

                1753    1262     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       4948
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3608    1239 
   Size:       2369    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3073    3938    4236    3073 
   Size:        865     298    3785

  Fragments arranged by size: 

                3785     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3611     882 
   Size:        617    2112    2219

  Fragments arranged by size: 

                2219    2112     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2739     493 
   Size:        711    1535    2702

  Fragments arranged by size: 

                2702    1535     711
 
BsaBI GATnn'nnATC

Cuts at:   4942    4942 
   Size:       4948
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2755    3137    3772     275 
   Size:         53      83     186     936    1222     382     635    1451

  Fragments arranged by size: 

                1451    1222     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2030    3381    3779    4053    4200    4611    2030 
   Size:       1351     398     274     147     411    2367

  Fragments arranged by size: 

                2367    1351     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4723

  Fragments arranged by size: 

                4723     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2111    2241    2244    1186 
   Size:        925     130       3    3890

  Fragments arranged by size: 

                3890     925     130       3
 
BseYI C'CCAG_C

Cuts at:   4151    4647    4151 
   Size:        496    4452
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4375
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2398    3159    4187    1540 
   Size:        275     186     397     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     397     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3009    3094    4165    3009 
   Size:         85    1071    3792

  Fragments arranged by size: 

                3792    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2459    2552    2459 
   Size:         93    4855
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2267    3009    3094    4165    1391 
   Size:        399     477     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     477     399      85
 
BspEI T'CCGG_A

Cuts at:   4611    4611 
   Size:       4948
 
BspHI T'CATG_A

Cuts at:   2837    4567    2837 
   Size:       1730    3218
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2236    2303     844 
   Size:       1392      67    3489

  Fragments arranged by size: 

                3489    1392      67
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2835    1394 
   Size:        429    1012    3507

  Fragments arranged by size: 

                3507    1012     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3443    3617      65 
   Size:       1183    2195     174    1396

  Fragments arranged by size: 

                2195    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3592    3779    1700 
   Size:        110    1782     187    2869

  Fragments arranged by size: 

                2869    1782     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       4948
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       4948
 
BssSI C'ACGA_G

Cuts at:   3008    4020    3008 
   Size:       1012    3936
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       4948
 
BstBI TT'CG_AA

Cuts at:   3752    3752 
   Size:       4948
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       4948
 
BstYI r'GATC_y

Cuts at:   1972    2278    2645    3030    3047    4499    4585    4597    4943 
   Size:        306     367     385      17    1452      86      12     346

Cuts at:   4943    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     367     346     306      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4396
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4209

  Fragments arranged by size: 

                4209     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2464    3258    3278    2464 
   Size:        794      20    4134

  Fragments arranged by size: 

                4134     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       4948
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4913
 
DraI TTT'AAA

Cuts at:   2252    2252 
   Size:       4948
 
DraIII CAC_nnn'GTG

Cuts at:   2212    2212 
   Size:       4948
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3955     809 
   Size:        507    2639    1802

  Fragments arranged by size: 

                2639    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2395    3284    4913 
   Size:         54    1425      50     275      18     565     889    1629

Cuts at:   4913       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     565     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2395    1537 
   Size:        275     583    4090

  Fragments arranged by size: 

                4090     583     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2878    1353 
   Size:       1525    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3535    3909    4055     387 
   Size:       1064     138    1946     374     146    1280

  Fragments arranged by size: 

                1946    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3011    3608    4395    4663    1239 
   Size:        393    1379     597     787     268    1524

  Fragments arranged by size: 

                1524    1379     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2257    2635    2257 
   Size:        378    4570
 
FspI TGC'GCA

Cuts at:   3454    3454 
   Size:       4948
 
HaeII r_GCGC'y

Cuts at:   1678    4095    1678 
   Size:       2417    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3589    3621    3663    3695    1373 
   Size:         32     135      32    2017      32      42      32    2626

  Fragments arranged by size: 

                2626    2017     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2292    2538     669 
   Size:        988     281     354     246    3079

  Fragments arranged by size: 

                3079     988     354     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       4948
 
HpaI GTT'AAC

Cuts at:   2538    2538 
   Size:       4948
 
KpnI G_GTAC'C

Cuts at:   2273    2273 
   Size:       4948
 
MfeI C'AATT_G

Cuts at:   2547    2547 
   Size:       4948
 
MluI A'CGCG_T

Cuts at:   4824    4824 
   Size:       4948
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4062    4246    1305 
   Size:       2757     184    2007

  Fragments arranged by size: 

                2757    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3126

  Fragments arranged by size: 

                3126    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2906    3265    3424     518 
   Size:        648    1740     359     159    2042

  Fragments arranged by size: 

                2042    1740     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2174    2364    3041    4189    4434    1606 
   Size:        568     190     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     568     245     190
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4396
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       4948
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       4948
 
NotI GC'GGCC_GC

Cuts at:   2395    2395 
   Size:       4948
 
NspI r_CATG'y

Cuts at:   1132    1229    2306    3851    1132 
   Size:         97    1077    1545    2229

  Fragments arranged by size: 

                2229    1545    1077      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       4948
 
PciI A'CATG_T

Cuts at:   3847    3847 
   Size:       4948
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       4948
 
PmeI GTTT'AAAC

Cuts at:   2252    2252 
   Size:       4948
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3017    3049    4530    4562    3017 
   Size:         32    1481      32    3403

  Fragments arranged by size: 

                3403    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       4948
 
PsiI TTA'TAA

Cuts at:   2518    2518 
   Size:       4948
 
PstI C_TGCA'G

Cuts at:    830    2300     830 
   Size:       1470    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       4948
 
PvuII CAG'CTG

Cuts at:   2174    2174 
   Size:       4948
 
SalI G'TCGA_C

Cuts at:   1936    2290    1936 
   Size:        354    4594
 
SbfI CC_TGCA'GG

Cuts at:   2300    2300 
   Size:       4948
 
ScaI AGT'ACT

Cuts at:   3196    4908    3196 
   Size:       1712    3236
 
SexAI A'CCwGG_T

Cuts at:   2208    2208 
   Size:       4948
 
SfcI C'TryA_G

Cuts at:    826    1045    2061    2137    2296    3431    4112    4303     826 
   Size:        219    1016      76     159    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1016     681     219     191     159      76
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4948
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       4948
 
SmaI CCC'GGG

Cuts at:   1872    2275    1872 
   Size:        403    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3052    3953    4215     820 
   Size:        197     926    1109     901     262    1553

  Fragments arranged by size: 

                1553    1109     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2739     493 
   Size:       2246    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       4948
 
SphI G_CATG'C

Cuts at:   1132    2306    1132 
   Size:       1174    3774
 
SspI AAT'ATT

Cuts at:      5      52    2872       5 
   Size:         47    2820    2081

  Fragments arranged by size: 

                2820    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       4948
 
StyI C'CwwG_G

Cuts at:    513    1065    2387     513 
   Size:        552    1322    3074

  Fragments arranged by size: 

                3074    1322     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2975    2992    3145    3330    3775     325 
   Size:       1255    1395      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1395    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3194    4906      96 
   Size:        274      80      33      51    1443    1217    1712     138

  Fragments arranged by size: 

                1712    1443    1217     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4908    4940    4908 
   Size:         32    4916
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       4948
 
XbaI T'CTAG_A

Cuts at:   2284    2284 
   Size:       4948
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       4948
 
XmaI C'CCGG_G

Cuts at:   1870    2273    1870 
   Size:        403    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3077    3077 
   Size:       4948
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2756     276 
   Size:         53      83     186     936    1222    2468

  Fragments arranged by size: 

                2468    1222     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI     SfiI     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com