################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BclI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 481 GGTACC G^GTACC AccI 381 GTCTAC GT^[AC][GT]AC Alw44I 1084 GTGCAC G^TGCAC AlwNI 718 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 860 AGCGCT AGC^GCT ApaLI 1084 GTGCAC G^TGCAC Asp718I 481 GGTACC G^GTACC AspHI 1084 GTGCAC G[AT]GC[AT]^C AvaI 344 CCCGAG C^[CT]CG[GA]G BbvCI 737 CCTCAGC CCTCAGC(-5/-2) BcgI 504 CGAGCGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 899 GTATCC GTATCC(6/5) BglI 181 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 128 AGATCT A^GATCT BsaWI 1256 TCCGGT [AT]^CCGG[AT] BseRI 1123 GAGGAG GAGGAG(10/8) BsiEI 228 CGGCCG CG[GA][CT]^CG BsiHKAI 1084 GTGCAC G[AT]GC[AT]^C BsoBI 344 CCCGAG C^[CT]CG[GA]G Bsp1407I 785 TGTACA T^GTACA BsrGI 785 TGTACA T^GTACA CfoI 861 GCGC GCG^C DsaI 485 CCATGG C^C[GA][CT]GG EaeI 228 CGGCCG [CT]^GGCC[GA] EagI 228 CGGCCG C^GGCCG EclXI 228 CGGCCG C^GGCCG Eco47III 860 AGCGCT AGC^GCT Eco52I 228 CGGCCG C^GGCCG EcoRV 1252 GATATC GAT^ATC FauI 879 GCGGG CCCGC(4/6) HaeII 860 AGCGCT [GA]GCGC^[CT] HhaI 861 GCGC GCG^C HinP1I 861 GCGC G^CGC HincII 111 GTTGAC GT[CT]^[GA]AC HindII 111 GTTGAC GT[CT]^[GA]AC HindIII 592 AAGCTT A^AGCTT KpnI 481 GGTACC GGTAC^C NcoI 485 CCATGG C^CATGG NotI 227 GCGGCCGC GC^GGCCGC PflMI 717 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1006 GACTC GAGTC(4/5) PstI 512 CTGCAG CTGCA^G SapI 331 GAAGAGC GCTCTTC(1/4) SphI 729 GCATGC GCATG^C SspBI 785 TGTACA T^GTACA TfiI 920 GATTC G^A[AT]TC Van91I 717 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1169 CCAGTGTGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwI 771 GGATC GGATC(4/5) 1266 GGATC ApaI 360 GGGCCC GGGCC^C 491 GGGCCC BbvI 733 GCAGC GCAGC(8/12) 831 GCTGC BfaI 49 CTAG C^TAG 1054 CTAG BpmI 1208 CTCCAG CTGGAG(16/14) 1180 CTGGAG BsrBI 225 GAGCGG CCGCTC(-3/-3) 505 GAGCGG BsrI 115 ACTGG ACTGG(1/-1) 1169 CCAGT BstEII 712 GGTTACC G^GT[ACGT]ACC 1164 GGTGACC BstPI 712 GGTTACC G^GT[ACGT]ACC 1164 GGTGACC BstXI 365 CCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 434 CCACTTATGTGG BstYI 128 AGATCT [GA]^GATC[CT] 1266 GGATCT EcoO65I 712 GGTTACC G^GT[ACGT]ACC 1164 GGTGACC HapII 876 CCGG C^CGG 1257 CCGG HinfI 920 GATTC G^A[ACGT]TC 1006 GACTC HpaII 876 CCGG C^CGG 1257 CCGG MaeI 49 CTAG C^TAG 1054 CTAG MflI 128 AGATCT [GA]^GATC[CT] 1266 GGATCT MseI 683 TTAA T^TAA 950 TTAA MspI 876 CCGG C^CGG 1257 CCGG MwoI 181 GCCAAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 733 GCAGCCTCAGC NspI 729 GCATGC [GA]CATG^[CT] 1025 GCATGT PpuMI 339 AGGTCCC [GA]G^G[AT]CC[CT] 803 GGGACCT SfcI 383 CTACAG C^T[GA][CT]AG 512 CTGCAG SmlI 1031 CTCAAG C^T[CT][GA]AG 1048 CTCAAG TaqI 503 TCGA T^CGA 521 TCGA Tru9I 683 TTAA T^TAA 950 TTAA TseI 733 GCAGC G^C[AT]GC 831 GCTGC Tsp45I 1063 GTCAC ^GT[GC]AC 1165 GTGAC TthHB8I 503 TCGA T^CGA 521 TCGA XhoII 128 AGATCT [GA]^GATC[CT] 1266 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BanI BanII BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tsp509I TspRI