(Circular) (Six-base) MAPSORT of: pFN21AE3185.seq Check: 6574 from: 1 to: 5980 pFN21AE3185 5980 bp With 182 enzymes: SgfI * March 28, 2012 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2387 3323 1937 Size: 450 936 4594 Fragments arranged by size: 4594 936 450 Acc65I G'GTAC_C Cuts at: 2486 3301 2486 Size: 815 5165 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3296 4043 5427 5695 1632 Size: 1664 747 1384 268 1917 Fragments arranged by size: 1917 1664 1384 747 268 AfeI AGC'GCT Cuts at: 2867 2867 Size: 5980 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 2243 2375 2444 1166 Size: 1077 132 69 4702 Fragments arranged by size: 4702 1077 132 69 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2852 1184 Size: 1668 4312 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3248 3280 3248 Size: 32 5948 AlwNI CAG_nnn'CTG Cuts at: 2728 3723 5295 2728 Size: 995 1572 3413 Fragments arranged by size: 3413 1572 995 ApaI G_GGCC'C Cuts at: 2369 2500 2369 Size: 131 5849 ApaLI G'TGCA_C Cuts at: 3089 4037 5193 3089 Size: 948 1156 3876 Fragments arranged by size: 3876 1156 948 ApoI r'AATT_y Cuts at: 1493 2045 2165 2963 3289 3515 3667 5776 1493 Size: 552 120 798 326 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 552 326 226 152 120 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2349 3305 1870 Size: 73 406 956 4545 Fragments arranged by size: 4545 956 406 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2486 2995 3301 4756 Size: 325 217 627 396 303 509 306 1455 Cuts at: 4756 618 Size: 1842 Fragments arranged by size: 1842 1455 627 509 396 325 306 303 217 BanII G_rGCy'C Cuts at: 1391 2369 2500 2714 3299 1391 Size: 978 131 214 585 4072 Fragments arranged by size: 4072 978 585 214 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5009 BbvCI CC'TCA_GC Cuts at: 2743 2743 Size: 5980 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2498 2532 4171 4205 1903 Size: 34 561 34 1639 34 3678 Fragments arranged by size: 3678 1639 561 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2915 3872 5082 2915 Size: 957 1210 3813 Fragments arranged by size: 3813 1210 957 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 4591 136 Size: 107 122 71 631 605 520 2399 1525 Fragments arranged by size: 2399 1525 631 605 520 122 107 71 BglII A'GATC_T Cuts at: 2133 5975 2133 Size: 3842 2138 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2369 2500 2998 3093 4041 5197 1790 Size: 579 131 498 95 948 1156 2573 Fragments arranged by size: 2573 1156 948 579 498 131 95 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2554 2554 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3198 3206 4640 1239 Size: 1959 8 1434 2579 Fragments arranged by size: 2579 1959 1434 8 Bpu10I CC'TnA_GC Cuts at: 2743 2743 Size: 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3021 3038 4105 4970 5268 3021 Size: 17 1067 865 298 3733 Fragments arranged by size: 3733 1067 865 298 17 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3261 4413 4811 5085 5232 5643 3261 Size: 1152 398 274 147 411 3598 Fragments arranged by size: 3598 1152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2796 2826 3122 3152 1178 Size: 30 165 30 1393 30 296 30 4006 Fragments arranged by size: 4006 1393 296 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3143 1186 Size: 1957 4023 BseYI C'CCAG_C Cuts at: 2700 5183 5679 2700 Size: 2483 496 3001 Fragments arranged by size: 3001 2483 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2236 3430 4191 5219 1540 Size: 275 186 235 1194 761 1028 2301 Fragments arranged by size: 2301 1194 1028 761 275 235 186 BsiHKAI G_wGCw'C Cuts at: 3093 4041 4126 5197 3093 Size: 948 85 1071 3876 Fragments arranged by size: 3876 1071 948 85 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2500 2714 2998 3093 3299 4041 Size: 399 579 131 214 284 95 206 742 Cuts at: 4041 4126 5197 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 399 284 214 206 131 95 85 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2232 2512 3867 1394 Size: 429 409 280 1355 3507 Fragments arranged by size: 3507 1355 429 409 280 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 2790 96 Size: 2694 3286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 2717 3169 1184 Size: 1533 452 3995 Fragments arranged by size: 3995 1533 452 BstXI CCAn_nnnn'nTGG Cuts at: 2377 2446 2377 Size: 69 5911 BstYI r'GATC_y Cuts at: 1972 2133 3271 3310 3677 4062 4079 5531 5617 Size: 161 1138 39 367 385 17 1452 86 Cuts at: 5617 5629 5975 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 161 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2490 513 Size: 552 939 486 4003 Fragments arranged by size: 4003 939 552 486 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 2424 3111 3227 3496 4290 4310 2424 Size: 687 116 269 794 20 4094 Fragments arranged by size: 4094 794 687 269 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2029 2064 529 Size: 35 1465 35 4445 Fragments arranged by size: 4445 1465 35 35 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 3427 4316 Size: 54 1425 50 275 18 403 1194 889 Cuts at: 4316 5945 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1194 889 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2233 3427 1537 Size: 275 421 1194 4090 Fragments arranged by size: 4090 1194 421 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 2331 2523 3910 1353 Size: 654 324 192 1387 3423 Fragments arranged by size: 3423 1387 654 324 192 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3198 3206 3296 4043 4640 5427 5695 Size: 393 1566 8 90 747 597 787 268 Cuts at: 5695 1239 Size: 1524 Fragments arranged by size: 1566 1524 787 747 597 393 268 90 8 EcoO109I rG'GnC_Cy Cuts at: 2345 2366 2496 2497 2548 2809 2345 Size: 21 130 1 51 261 5516 Fragments arranged by size: 5516 261 130 51 21 1 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 EcoRV GAT'ATC Cuts at: 3259 3259 Size: 5980 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2869 5127 1678 Size: 1191 2258 2531 Fragments arranged by size: 2531 2258 1191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2602 2634 3122 3154 4621 Size: 32 135 32 1030 32 488 32 1467 Cuts at: 4621 4653 4695 4727 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1467 1030 488 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2118 3324 3570 669 Size: 988 281 180 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 180 HindIII A'AGCT_T Cuts at: 748 2597 748 Size: 1849 4131 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 2490 3305 2490 Size: 815 5165 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2088 5094 5278 1305 Size: 783 3006 184 2007 Fragments arranged by size: 3006 2007 783 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2270 2375 2444 3167 3938 4297 Size: 648 1077 27 105 69 723 771 359 Cuts at: 4297 4456 518 Size: 159 2042 Fragments arranged by size: 2042 1077 771 723 648 359 159 105 69 27 MspA1I CmG'CkG Cuts at: 1606 3396 4073 5221 5466 1606 Size: 1790 677 1148 245 2120 Fragments arranged by size: 2120 1790 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2490 513 Size: 552 939 486 4003 Fragments arranged by size: 4003 939 552 486 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 2233 3427 2233 Size: 1194 4786 NspI r_CATG'y Cuts at: 1132 1229 2738 3034 3338 4883 1132 Size: 97 1509 296 304 1545 2229 Fragments arranged by size: 2229 1545 1509 304 296 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 2728 1108 Size: 1620 4360 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2345 2809 2345 Size: 464 5516 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 2604 3550 2604 Size: 946 5034 PspOMI G'GGCC_C Cuts at: 2365 2496 2365 Size: 131 5849 PstI C_TGCA'G Cuts at: 830 2521 3332 830 Size: 1691 811 3478 Fragments arranged by size: 3478 1691 811 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SapI GCTCTTCn'nnn_ Cuts at: 2331 2331 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 2388 2517 3328 4463 5144 5335 826 Size: 219 1343 129 811 1135 681 191 1471 Fragments arranged by size: 1471 1343 1135 811 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3036 3053 4084 4985 5247 820 Size: 197 926 1093 17 1031 901 262 1553 Fragments arranged by size: 1553 1093 1031 926 901 262 197 17 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 2738 3338 1132 Size: 1606 600 3774 Fragments arranged by size: 3774 1606 600 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 2259 2301 2490 2871 3419 Size: 552 939 183 72 42 189 381 548 Cuts at: 3419 513 Size: 3074 Fragments arranged by size: 3074 939 552 548 381 189 183 72 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3013 4007 4024 4177 4362 4807 325 Size: 1255 1433 994 17 153 185 445 1498 Fragments arranged by size: 1498 1433 1255 994 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2790 4226 5938 Size: 274 80 33 51 1443 813 1436 1712 Cuts at: 5938 96 Size: 138 Fragments arranged by size: 1712 1443 1436 813 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2988 3020 5940 5972 2988 Size: 32 2920 32 2996 Fragments arranged by size: 2996 2920 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3181 3181 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI