(Linear) (Six-base) MAPSORT of: KIEE3185.seq Check: 5649 from: 1 to: 1275 KIEE3185.orf With 182 enzymes: SgfI * March 22, 2012 16:19 .. AccI GT'mk_AC Cuts at: 0 382 1275 Size: 382 893 Acc65I G'GTAC_C Cuts at: 0 481 1275 Size: 481 794 AfeI AGC'GCT Cuts at: 0 862 1275 Size: 862 413 AleI CACnn'nnGTG Cuts at: 0 238 370 439 1275 Size: 238 132 69 836 Fragments arranged by size: 836 238 132 69 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 847 1275 Size: 847 428 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1243 1275 Size: 1243 32 AlwNI CAG_nnn'CTG Cuts at: 0 723 1275 Size: 723 552 ApaI G_GGCC'C Cuts at: 0 364 495 1275 Size: 364 131 780 Fragments arranged by size: 780 364 131 ApaLI G'TGCA_C Cuts at: 0 1084 1275 Size: 1084 191 ApoI r'AATT_y Cuts at: 0 40 160 958 1275 Size: 40 120 798 317 Fragments arranged by size: 798 317 120 40 AvaI C'yCGr_G Cuts at: 0 344 1275 Size: 344 931 BanI G'GyrC_C Cuts at: 0 178 481 990 1275 Size: 178 303 509 285 Fragments arranged by size: 509 303 285 178 BanII G_rGCy'C Cuts at: 0 364 495 709 1275 Size: 364 131 214 566 Fragments arranged by size: 566 364 214 131 BbvCI CC'TCA_GC Cuts at: 0 738 1275 Size: 738 537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 527 1275 Size: 493 34 748 Fragments arranged by size: 748 493 34 BciVI GTATCCnnnnn_n' Cuts at: 0 910 1275 Size: 910 365 BglI GCCn_nnn'nGGC Cuts at: 0 187 1275 Size: 187 1088 BglII A'GATC_T Cuts at: 0 128 1275 Size: 128 1147 Bme1580I G_kGCm'C Cuts at: 0 364 495 993 1088 1275 Size: 364 131 498 95 187 Fragments arranged by size: 498 364 187 131 95 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 549 1275 Size: 549 726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1193 1201 1275 Size: 1193 8 74 Fragments arranged by size: 1193 74 8 Bpu10I CC'TnA_GC Cuts at: 0 738 1275 Size: 738 537 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1016 1033 1275 Size: 1016 17 242 Fragments arranged by size: 1016 242 17 BsaWI w'CCGG_w Cuts at: 0 1256 1275 Size: 1256 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 791 821 1117 1147 1275 Size: 791 30 296 30 128 Fragments arranged by size: 791 296 128 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1138 1275 Size: 1138 137 BseYI C'CCAG_C Cuts at: 0 695 1275 Size: 695 580 BsiEI CG_ry'CG Cuts at: 0 231 1275 Size: 231 1044 BsiHKAI G_wGCw'C Cuts at: 0 1088 1275 Size: 1088 187 Bsp1286I G_dGCh'C Cuts at: 0 364 495 709 993 1088 1275 Size: 364 131 214 284 95 187 Fragments arranged by size: 364 284 214 187 131 95 BsrBI CCG'CTC Cuts at: 0 227 507 1275 Size: 227 280 768 Fragments arranged by size: 768 280 227 BsrGI T'GTAC_A Cuts at: 0 785 1275 Size: 785 490 BstEII G'GTnAC_C Cuts at: 0 712 1164 1275 Size: 712 452 111 Fragments arranged by size: 712 452 111 BstXI CCAn_nnnn'nTGG Cuts at: 0 372 441 1275 Size: 372 69 834 Fragments arranged by size: 834 372 69 BstYI r'GATC_y Cuts at: 0 128 1266 1275 Size: 128 1138 9 Fragments arranged by size: 1138 128 9 BtgI C'CryG_G Cuts at: 0 485 1275 Size: 485 790 BtsI GCAGTG_nn' Cuts at: 0 419 1106 1222 1275 Size: 419 687 116 53 Fragments arranged by size: 687 419 116 53 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 24 59 1275 Size: 24 35 1216 Fragments arranged by size: 1216 35 24 EaeI y'GGCC_r Cuts at: 0 228 1275 Size: 228 1047 EagI C'GGCC_G Cuts at: 0 228 1275 Size: 228 1047 EarI CTCTTCn'nnn_ Cuts at: 0 2 326 518 1275 Size: 2 324 192 757 Fragments arranged by size: 757 324 192 2 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1193 1201 1275 Size: 1193 8 74 Fragments arranged by size: 1193 74 8 EcoO109I rG'GnC_Cy Cuts at: 0 340 361 491 492 543 804 1275 Size: 340 21 130 1 51 261 471 Fragments arranged by size: 471 340 261 130 51 21 1 EcoRV GAT'ATC Cuts at: 0 1254 1275 Size: 1254 21 HaeII r_GCGC'y Cuts at: 0 864 1275 Size: 864 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 597 629 1117 1149 1275 Size: 597 32 488 32 126 Fragments arranged by size: 597 488 126 32 32 HincII GTy'rAC Cuts at: 0 113 1275 Size: 113 1162 HindIII A'AGCT_T Cuts at: 0 592 1275 Size: 592 683 KpnI G_GTAC'C Cuts at: 0 485 1275 Size: 485 790 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 83 1275 Size: 83 1192 MslI CAynn'nnrTG Cuts at: 0 238 265 370 439 1162 1275 Size: 238 27 105 69 723 113 Fragments arranged by size: 723 238 113 105 69 27 NcoI C'CATG_G Cuts at: 0 485 1275 Size: 485 790 NotI GC'GGCC_GC Cuts at: 0 228 1275 Size: 228 1047 NspI r_CATG'y Cuts at: 0 733 1029 1275 Size: 733 296 246 Fragments arranged by size: 733 296 246 PflMI CCAn_nnn'nTGG Cuts at: 0 723 1275 Size: 723 552 PpuMI rG'GwC_Cy Cuts at: 0 340 804 1275 Size: 340 464 471 Fragments arranged by size: 471 464 340 PsiI TTA'TAA Cuts at: 0 599 1275 Size: 599 676 PspOMI G'GGCC_C Cuts at: 0 360 491 1275 Size: 360 131 784 Fragments arranged by size: 784 360 131 PstI C_TGCA'G Cuts at: 0 516 1275 Size: 516 759 SapI GCTCTTCn'nnn_ Cuts at: 0 326 1275 Size: 326 949 SfcI C'TryA_G Cuts at: 0 383 512 1275 Size: 383 129 763 Fragments arranged by size: 763 383 129 SmlI C'TyrA_G Cuts at: 0 1031 1048 1275 Size: 1031 17 227 Fragments arranged by size: 1031 227 17 SphI G_CATG'C Cuts at: 0 733 1275 Size: 733 542 StyI C'CwwG_G Cuts at: 0 182 254 296 485 866 1275 Size: 182 72 42 189 381 409 Fragments arranged by size: 409 381 189 182 72 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1008 1275 Size: 1008 267 TatI w'GTAC_w Cuts at: 0 785 1275 Size: 785 490 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 983 1015 1275 Size: 983 32 260 Fragments arranged by size: 983 260 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1176 1275 Size: 1176 99 Enzymes that do cut: AccI Acc65I AfeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbvCI BcgI BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrGI BstEII BstXI BstYI BtgI BtsI CspCI EaeI EagI EarI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII KpnI MmeI MslI NcoI NotI NspI PflMI PpuMI PsiI PspOMI PstI SapI SfcI SmlI SphI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AclI AcuI AflII AflIII AgeI AhdI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BclI BfrBI BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HpaI KasI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI