Restriction map for
pFN21AB7668
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI ApoI AscI AseI
AsnI Asp718I AspEI BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BpuAI BsaAI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI DraI
DraII DraIII DrdI Eam1105I EarI
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MfeI MflI MluI MluNI MscI
MseI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SfiI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 755 TCCGGA T^CCGGA
Alw44I 7 GTGCAC G^TGCAC
Aor51HI 972 AGCGCT AGC^GCT
ApaI 1114 GGGCCC GGGCC^C
ApaLI 7 GTGCAC G^TGCAC
AspI 477 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 230 TGCGCA TGC^GCA
AvrII 729 CCTAGG C^CTAGG
BbeI 733 GGCGCC GGCGC^C
BfaI 730 CTAG C^TAG
BglI 1067 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 729 CCTAGG C^CTAGG
BlpI 720 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 720 GCTGAGC GC^T[ACGT]AGC
BsaI 646 GAGACC GGTCTC(1/5)
BsaWI 755 TCCGGA [AT]^CCGG[AT]
BseAI 755 TCCGGA T^CCGGA
BsgI 1081 CTGCAC GTGCAG(16/14)
BspEI 755 TCCGGA T^CCGGA
BspMI 271 ACCTGC ACCTGC(4/8)
BssHII 200 GCGCGC G^CGCGC
CelII 720 GCTGAGC GC^T[ACGT]AGC
CpoI 410 CGGACCG CG^G[AT]CCG
DpnII 1032 GATC ^GATC
Eco47III 972 AGCGCT AGC^GCT
EcoNI 372 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 230 TGCGCA TGC^GCA
KasI 733 GGCGCC G^GCGCC
MaeI 730 CTAG C^TAG
MaeIII 284 GTAAC ^GT[ACGT]AC
MboI 1032 GATC ^GATC
MroI 755 TCCGGA T^CCGGA
NarI 733 GGCGCC GG^CGCC
NdeII 1032 GATC ^GATC
NotI 785 GCGGCCGC GC^GGCCGC
PflFI 477 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1160 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 445 CTGCAG CTGCA^G
RsrII 410 CGGACCG CG^G[AT]CCG
Sau3AI 1032 GATC ^GATC
SexAI 224 ACCTGGT A^CC[AT]GGT
SfoI 733 GGCGCC GGC^GCC
SgrAI 300 CACCGGCG C[GA]^CCGG[CT]G
SphI 770 GCATGC GCATG^C
StuI 1065 AGGCCT AGG^CCT
Tth111I 477 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 733 GGCGCC G[GA]^CG[CT]C
960 GACGCC
AfaI 407 GTAC GT^AC
825 GTAC
Asp700I 123 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
330 GAACAAGTTC
BsaHI 733 GGCGCC G[GA]^CG[CT]C
960 GACGCC
BseRI 307 GAGGAG GAGGAG(10/8)
514 GAGGAG
BsmFI 423 GTCCC GGGAC(10/14)
915 GTCCC
BstAPI 182 GCACGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1083 GCACCCCATGC
DsaI 763 CCGCGG C^C[GA][CT]GG
1004 CCGCGG
EagI 786 CGGCCG C^GGCCG
920 CGGCCG
EclXI 786 CGGCCG C^GGCCG
920 CGGCCG
Eco52I 786 CGGCCG C^GGCCG
920 CGGCCG
EcoT14I 729 CCTAGG C^C[AT][AT]GG
846 CCAAGG
Hin1I 733 GGCGCC G[GA]^CG[CT]C
960 GACGCC
KspI 763 CCGCGG CCGC^GG
1004 CCGCGG
MaeII 56 ACGT A^CGT
317 ACGT
MboII 752 TCTTC GAAGA(8/7)
884 TCTTC
NaeI 193 GCCGGC GCC^GGC
991 GCCGGC
NgoMIV 193 GCCGGC G^CCGGC
991 GCCGGC
NspI 659 ACATGC [GA]CATG^[CT]
770 GCATGC
RsaI 407 GTAC GT^AC
825 GTAC
SacII 763 CCGCGG CCGC^GG
1004 CCGCGG
SfaNI 209 GCATC GCATC(5/9)
1170 GCATC
SfcI 445 CTGCAG C^T[GA][CT]AG
1152 CTACAG
SmlI 373 CTCAAG C^T[CT][GA]AG
583 CTCAAG
StyI 729 CCTAGG C^C[AT][AT]GG
846 CCAAGG
XmnI 123 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
330 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PvuII
SacI Sau96I ScrFI TaqI TseI
TspRI TthHB8I