(Circular) (Six-base) MAPSORT of: pFN21AB7668.seq Check: 2598 from: 1 to: 5911 pFN21AB7668 5911 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2285 2285 Size: 5911 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3254 1937 Size: 1317 4594 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3974 5358 5626 1632 Size: 2342 1384 268 1917 Fragments arranged by size: 2342 1917 1384 268 AfeI AGC'GCT Cuts at: 2979 2979 Size: 5911 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2684 2716 2684 Size: 32 5879 AlwNI CAG_nnn'CTG Cuts at: 3654 5226 3654 Size: 1572 4339 ApaI G_GGCC'C Cuts at: 3123 3123 Size: 5911 ApaLI G'TGCA_C Cuts at: 2012 3968 5124 2012 Size: 1956 1156 2799 Fragments arranged by size: 2799 1956 1156 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2530 3062 3185 3236 1870 Size: 73 587 532 123 51 4545 Fragments arranged by size: 4545 587 532 123 73 51 AvrII C'CTAG_G Cuts at: 2734 2734 Size: 5911 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 2738 3146 3232 4687 Size: 325 217 627 747 204 408 86 1455 Cuts at: 4687 618 Size: 1842 Fragments arranged by size: 1842 1455 747 627 408 325 217 204 86 BanII G_rGCy'C Cuts at: 1391 2319 2369 3062 3123 3192 3230 1391 Size: 928 50 693 61 69 38 4072 Fragments arranged by size: 4072 928 693 69 61 50 38 BbeI G_GCGC'C Cuts at: 2742 2742 Size: 5911 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4940 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3078 4522 136 Size: 107 122 71 631 605 1406 1444 1525 Fragments arranged by size: 1525 1444 1406 631 605 122 107 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 2726 3328 2726 Size: 602 5309 Bme1580I G_kGCm'C Cuts at: 1790 2016 2537 3083 3123 3972 5128 1790 Size: 226 521 546 40 849 1156 2573 Fragments arranged by size: 2573 1156 849 546 521 226 40 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4600 442 Size: 416 53 83 399 24 2225 958 1753 Fragments arranged by size: 2225 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2545 2545 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2600 2917 4571 1239 Size: 1184 177 317 1654 2579 Fragments arranged by size: 2579 1654 1184 317 177 Bpu10I CC'TnA_GC Cuts at: 2363 2363 Size: 5911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 2573 4036 4901 5199 2363 Size: 210 1463 865 298 3075 Fragments arranged by size: 3075 1463 865 298 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2645 4574 882 Size: 617 1146 1929 2219 Fragments arranged by size: 2219 1929 1146 617 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 2966 3718 4100 Size: 53 83 186 936 1206 227 752 382 Cuts at: 4100 4735 275 Size: 635 1451 Fragments arranged by size: 1451 1206 936 752 635 382 227 186 83 53 BsaWI w'CCGG_w Cuts at: 2760 4344 4742 5016 5163 5574 2760 Size: 1584 398 274 147 411 3097 Fragments arranged by size: 3097 1584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5686 Fragments arranged by size: 5686 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 2534 1186 Size: 1141 207 4563 Fragments arranged by size: 4563 1141 207 BseYI C'CCAG_C Cuts at: 2222 2785 2971 3122 5114 5610 2222 Size: 563 186 151 1992 496 2523 Fragments arranged by size: 2523 1992 563 496 186 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3071 1163 Size: 573 1335 4003 Fragments arranged by size: 4003 1335 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 2794 2928 3361 4122 5150 Size: 275 186 46 747 134 433 761 1028 Cuts at: 5150 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 747 433 275 186 134 46 BsiHKAI G_wGCw'C Cuts at: 2016 2319 2369 3192 3972 4057 5128 2016 Size: 303 50 823 780 85 1071 2799 Fragments arranged by size: 2799 1071 823 780 303 85 50 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 BsmBI CGTCTCn'nnnn_ Cuts at: 2462 2492 2576 2462 Size: 30 84 5797 Fragments arranged by size: 5797 84 30 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2319 2369 2537 3062 3083 3123 Size: 399 226 303 50 168 525 21 40 Cuts at: 3123 3192 3230 3972 4057 5128 1391 Size: 69 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 525 399 303 226 168 85 69 50 40 38 21 BspEI T'CCGG_A Cuts at: 2760 5574 2760 Size: 2814 3097 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2285 3266 844 Size: 1441 981 3489 Fragments arranged by size: 3489 1441 981 BsrBI CCG'CTC Cuts at: 1394 1823 2032 2992 3144 3798 1394 Size: 429 209 960 152 654 3507 Fragments arranged by size: 3507 960 654 429 209 152 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2198 2306 2654 2996 4555 4742 Size: 110 211 177 108 348 342 1559 187 Cuts at: 4742 1700 Size: 2869 Fragments arranged by size: 2869 1559 348 342 211 187 177 110 108 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 2205 1925 Size: 280 5631 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 3094 794 Size: 1399 901 3611 Fragments arranged by size: 3611 1399 901 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2578 2941 2230 Size: 348 363 5200 Fragments arranged by size: 5200 363 348 BstYI r'GATC_y Cuts at: 1972 3241 3608 3993 4010 5462 5548 5560 5906 Size: 1269 367 385 17 1452 86 12 346 Cuts at: 5906 1972 Size: 1977 Fragments arranged by size: 1977 1452 1269 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2768 3009 513 Size: 552 1703 241 3415 Fragments arranged by size: 3415 1703 552 241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2014 2447 3427 4221 4241 2014 Size: 433 980 794 20 3684 Fragments arranged by size: 3684 980 794 433 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2502 2791 2925 Size: 54 1425 50 275 18 672 289 134 Cuts at: 2925 3358 4247 5876 8 Size: 433 889 1629 43 Fragments arranged by size: 1629 1425 889 672 433 289 275 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2791 2925 3358 1537 Size: 275 979 134 433 4090 Fragments arranged by size: 4090 979 433 275 134 EarI CTCTTCn'nnn_ Cuts at: 1353 3841 1353 Size: 2488 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2317 2367 3190 2317 Size: 50 823 5038 Fragments arranged by size: 5038 823 50 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2600 2917 3974 4571 5358 5626 Size: 393 791 177 317 1057 597 787 268 Cuts at: 5626 1239 Size: 1524 Fragments arranged by size: 1524 1057 791 787 597 393 317 268 177 EcoNI CCTnn'n_nnAGG Cuts at: 2381 2381 Size: 5911 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 5911 FspI TGC'GCA Cuts at: 2237 4417 2237 Size: 2180 3731 HaeII r_GCGC'y Cuts at: 1678 2182 2733 2742 2981 5058 1678 Size: 504 551 9 239 2077 2531 Fragments arranged by size: 2531 2077 551 504 239 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3023 3055 4552 4584 4626 Size: 32 135 32 1451 32 1497 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 1451 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KasI G'GCGC_C Cuts at: 2738 2738 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 5025 5209 1305 Size: 1164 2556 184 2007 Fragments arranged by size: 2556 2007 1164 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4089 Fragments arranged by size: 4089 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2279 2678 3032 3869 4228 4387 Size: 648 843 270 399 354 837 359 159 Cuts at: 4387 518 Size: 2042 Fragments arranged by size: 2042 843 837 648 399 359 354 270 159 MspA1I CmG'CkG Cuts at: 1606 2299 2578 2641 2725 2770 2785 2971 3011 Size: 693 279 63 84 45 15 186 40 Cuts at: 3011 3114 3153 3162 3327 4004 5152 5397 1606 Size: 103 39 9 165 677 1148 245 2120 Fragments arranged by size: 2120 1148 693 677 279 245 186 165 103 84 63 45 40 39 15 9 NaeI GCC'GGC Cuts at: 2200 2998 2200 Size: 798 5113 NarI GG'CG_CC Cuts at: 2739 2739 Size: 5911 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NgoMIV G'CCGG_C Cuts at: 2198 2996 2198 Size: 798 5113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 2791 3358 2791 Size: 567 5344 NspI r_CATG'y Cuts at: 1132 1229 2668 2779 3269 4814 1132 Size: 97 1439 111 490 1545 2229 Fragments arranged by size: 2229 1545 1439 490 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PfoI T'CCnGG_A Cuts at: 2439 2439 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2324 2356 2976 3008 3980 4012 5493 5525 2324 Size: 32 620 32 972 32 1481 32 2710 Fragments arranged by size: 2710 1481 972 620 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3169 1613 Size: 1556 4355 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PspOMI G'GGCC_C Cuts at: 3119 3119 Size: 5911 PstI C_TGCA'G Cuts at: 830 2454 3263 830 Size: 1624 809 3478 Fragments arranged by size: 3478 1624 809 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2299 2578 2641 2725 2299 Size: 279 63 84 5485 Fragments arranged by size: 5485 279 84 63 RsrII CG'GwC_CG Cuts at: 2416 2416 Size: 5911 SacI G_AGCT'C Cuts at: 2319 2369 3192 2319 Size: 50 823 5038 Fragments arranged by size: 5038 823 50 SacII CC_GC'GG Cuts at: 2771 3012 2771 Size: 241 5670 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SexAI A'CCwGG_T Cuts at: 2229 2229 Size: 5911 SfcI C'TryA_G Cuts at: 826 1045 2450 3157 3259 4394 5075 5266 826 Size: 219 1405 707 102 1135 681 191 1471 Fragments arranged by size: 1471 1405 1135 707 681 219 191 102 SfoI GGC'GCC Cuts at: 2740 2740 Size: 5911 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 2306 1700 Size: 606 5305 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2378 2588 4015 4916 5178 820 Size: 197 926 435 210 1427 901 262 1553 Fragments arranged by size: 1553 1427 926 901 435 262 210 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 2779 3269 1132 Size: 1647 490 3774 Fragments arranged by size: 3774 1647 490 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 3072 1517 Size: 1555 4356 StyI C'CwwG_G Cuts at: 513 1065 2734 2851 3350 513 Size: 552 1669 117 499 3074 Fragments arranged by size: 3074 1669 552 499 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3938 3955 4108 4293 4738 325 Size: 1255 2358 17 153 185 445 1498 Fragments arranged by size: 2358 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5869 96 Size: 274 80 33 51 1443 2180 1712 138 Fragments arranged by size: 2180 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 5871 5903 2060 Size: 32 3779 32 2068 Fragments arranged by size: 3779 2068 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2485 1316 Size: 1169 4742 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 2132 2339 4040 2132 Size: 207 1701 4003 Fragments arranged by size: 4003 1701 207 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI SanDI SapI SfiI SrfI SwaI XcmI