Restriction map for pFN21AB7668
 (Linear) (Six-base) MAPSORT of: KIBB7668.seq  Check: 1870  from: 1  to: 1206



KIBB7668.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     280    1206 
   Size:        280     926
 
AfeI AGC'GCT

Cuts at:      0     974    1206 
   Size:        974     232
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     679     711    1206 
   Size:        679      32     495

  Fragments arranged by size: 

                 679     495      32
 
ApaI G_GGCC'C

Cuts at:      0    1118    1206 
   Size:       1118      88
 
ApaLI G'TGCA_C

Cuts at:      0       7    1206 
   Size:          7    1199
 
AvaI C'yCGr_G

Cuts at:      0     525    1057    1180    1206 
   Size:        525     532     123      26

  Fragments arranged by size: 

                 532     525     123      26
 
AvrII C'CTAG_G

Cuts at:      0     729    1206 
   Size:        729     477
 
BanI G'GyrC_C

Cuts at:      0     529     733    1141    1206 
   Size:        529     204     408      65

  Fragments arranged by size: 

                 529     408     204      65
 
BanII G_rGCy'C

Cuts at:      0     314     364    1057    1118    1187    1206 
   Size:        314      50     693      61      69      19

  Fragments arranged by size: 

                 693     314      69      61      50      19
 
BbeI G_GCGC'C

Cuts at:      0     737    1206 
   Size:        737     469
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1073    1206 
   Size:       1073     133
 
BlpI GC'TnA_GC

Cuts at:      0     721    1206 
   Size:        721     485
 
Bme1580I G_kGCm'C

Cuts at:      0      11     532    1078    1118    1206 
   Size:         11     521     546      40      88

  Fragments arranged by size: 

                 546     521      88      40      11
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     540    1206 
   Size:        540     666
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     595     912    1206 
   Size:        418     177     317     294

  Fragments arranged by size: 

                 418     317     294     177
 
Bpu10I CC'TnA_GC

Cuts at:      0     358    1206 
   Size:        358     848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     568    1206 
   Size:        358     210     638

  Fragments arranged by size: 

                 638     358     210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     640    1206 
   Size:        640     566
 
BsaHI Gr'CG_yC

Cuts at:      0     734     961    1206 
   Size:        734     227     245

  Fragments arranged by size: 

                 734     245     227
 
BsaWI w'CCGG_w

Cuts at:      0     755    1206 
   Size:        755     451
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     322     529    1206 
   Size:        322     207     677

  Fragments arranged by size: 

                 677     322     207
 
BseYI C'CCAG_C

Cuts at:      0     217     780     966    1117    1206 
   Size:        217     563     186     151      89

  Fragments arranged by size: 

                 563     217     186     151      89
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1066    1206 
   Size:       1066     140
 
BsiEI CG_ry'CG

Cuts at:      0      42     789     923    1206 
   Size:         42     747     134     283

  Fragments arranged by size: 

                 747     283     134      42
 
BsiHKAI G_wGCw'C

Cuts at:      0      11     314     364    1187    1206 
   Size:         11     303      50     823      19

  Fragments arranged by size: 

                 823     303      50      19      11
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     457     487     571    1206 
   Size:        457      30      84     635

  Fragments arranged by size: 

                 635     457      84      30
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     314     364     532    1057    1078    1118    1187 
   Size:         11     303      50     168     525      21      40      69

Cuts at:   1187    1206 
   Size:         19

  Fragments arranged by size: 

                 525     303     168      69      50      40      21      19
                  11
 
BspEI T'CCGG_A

Cuts at:      0     755    1206 
   Size:        755     451
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     280    1206 
   Size:        280     926
 
BsrBI CCG'CTC

Cuts at:      0      27     987    1139    1206 
   Size:         27     960     152      67

  Fragments arranged by size: 

                 960     152      67      27
 
BsrFI r'CCGG_y

Cuts at:      0      16     193     301     649     991    1206 
   Size:         16     177     108     348     342     215

  Fragments arranged by size: 

                 348     342     215     177     108      16
 
BssHII G'CGCG_C

Cuts at:      0     200    1206 
   Size:        200    1006
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     188    1089    1206 
   Size:        188     901     117

  Fragments arranged by size: 

                 901     188     117
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     225     573     936    1206 
   Size:        225     348     363     270

  Fragments arranged by size: 

                 363     348     270     225
 
BtgI C'CryG_G

Cuts at:      0     763    1004    1206 
   Size:        763     241     202

  Fragments arranged by size: 

                 763     241     202
 
BtsI GCAGTG_nn'

Cuts at:      0       9     442    1206 
   Size:          9     433     764

  Fragments arranged by size: 

                 764     433       9
 
EaeI y'GGCC_r

Cuts at:      0     497     786     920    1206 
   Size:        497     289     134     286

  Fragments arranged by size: 

                 497     289     286     134
 
EagI C'GGCC_G

Cuts at:      0     786     920    1206 
   Size:        786     134     286

  Fragments arranged by size: 

                 786     286     134
 
EcoICRI GAG'CTC

Cuts at:      0     312     362    1185    1206 
   Size:        312      50     823      21

  Fragments arranged by size: 

                 823     312      50      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     595     912    1206 
   Size:        418     177     317     294

  Fragments arranged by size: 

                 418     317     294     177
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     376    1206 
   Size:        376     830
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     609    1206 
   Size:        609     597
 
FspI TGC'GCA

Cuts at:      0     232    1206 
   Size:        232     974
 
HaeII r_GCGC'y

Cuts at:      0     177     728     737     976    1206 
   Size:        177     551       9     239     230

  Fragments arranged by size: 

                 551     239     230     177       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1018    1050    1206 
   Size:       1018      32     156

  Fragments arranged by size: 

                1018     156      32
 
KasI G'GCGC_C

Cuts at:      0     733    1206 
   Size:        733     473
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1206 
   Size:        464     742
 
MslI CAynn'nnrTG

Cuts at:      0     274     673    1027    1206 
   Size:        274     399     354     179

  Fragments arranged by size: 

                 399     354     274     179
 
MspA1I CmG'CkG

Cuts at:      0     294     573     636     720     765     780     966    1006 
   Size:        294     279      63      84      45      15     186      40

Cuts at:   1006    1109    1148    1157    1206 
   Size:        103      39       9      49

  Fragments arranged by size: 

                 294     279     186     103      84      63      49      45
                  40      39      15       9
 
NaeI GCC'GGC

Cuts at:      0     195     993    1206 
   Size:        195     798     213

  Fragments arranged by size: 

                 798     213     195
 
NarI GG'CG_CC

Cuts at:      0     734    1206 
   Size:        734     472
 
NgoMIV G'CCGG_C

Cuts at:      0     193     991    1206 
   Size:        193     798     215

  Fragments arranged by size: 

                 798     215     193
 
NotI GC'GGCC_GC

Cuts at:      0     786    1206 
   Size:        786     420
 
NspI r_CATG'y

Cuts at:      0     663     774    1206 
   Size:        663     111     432

  Fragments arranged by size: 

                 663     432     111
 
PfoI T'CCnGG_A

Cuts at:      0     434    1206 
   Size:        434     772
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     319     351     971    1003    1206 
   Size:        319      32     620      32     203

  Fragments arranged by size: 

                 620     319     203      32      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1164    1206 
   Size:       1164      42
 
PspOMI G'GGCC_C

Cuts at:      0    1114    1206 
   Size:       1114      92
 
PstI C_TGCA'G

Cuts at:      0     449    1206 
   Size:        449     757
 
PvuII CAG'CTG

Cuts at:      0     294     573     636     720    1206 
   Size:        294     279      63      84     486

  Fragments arranged by size: 

                 486     294     279      84      63
 
RsrII CG'GwC_CG

Cuts at:      0     411    1206 
   Size:        411     795
 
SacI G_AGCT'C

Cuts at:      0     314     364    1187    1206 
   Size:        314      50     823      19

  Fragments arranged by size: 

                 823     314      50      19
 
SacII CC_GC'GG

Cuts at:      0     766    1007    1206 
   Size:        766     241     199

  Fragments arranged by size: 

                 766     241     199
 
SexAI A'CCwGG_T

Cuts at:      0     224    1206 
   Size:        224     982
 
SfcI C'TryA_G

Cuts at:      0     445    1152    1206 
   Size:        445     707      54

  Fragments arranged by size: 

                 707     445      54
 
SfoI GGC'GCC

Cuts at:      0     735    1206 
   Size:        735     471
 
SgrAI Cr'CCGG_yG

Cuts at:      0     301    1206 
   Size:        301     905
 
SmlI C'TyrA_G

Cuts at:      0     373     583    1206 
   Size:        373     210     623

  Fragments arranged by size: 

                 623     373     210
 
SphI G_CATG'C

Cuts at:      0     774    1206 
   Size:        774     432
 
StuI AGG'CCT

Cuts at:      0    1067    1206 
   Size:       1067     139
 
StyI C'CwwG_G

Cuts at:      0     729     846    1206 
   Size:        729     117     360

  Fragments arranged by size: 

                 729     360     117
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      55      87    1206 
   Size:         55      32    1119

  Fragments arranged by size: 

                1119      55      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     480    1206 
   Size:        480     726
 
XmnI GAAnn'nnTTC

Cuts at:      0     127     334    1206 
   Size:        127     207     872

  Fragments arranged by size: 

                 872     207     127
 

 Enzymes that do cut:

     AarI     AfeI     AloI     ApaI    ApaLI     AvaI    AvrII     BanI
    BanII     BbeI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII
   BstAPI    BstXI     BtgI     BtsI     EaeI     EagI  EcoICRI  Eco57MI
    EcoNI     FalI     FspI    HaeII    Hin4I     KasI     MmeI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI     PfoI     PpiI
    PshAI   PspOMI     PstI    PvuII    RsrII     SacI    SacII    SexAI
     SfcI     SfoI    SgrAI     SmlI     SphI     StuI     StyI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApoI     AscI     AseI    AsiSI
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaXI    BsiWI
     BsmI    BspHI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI     EciI EcoO109I    EcoRI    EcoRV     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NcoI     NdeI
     NheI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI    PpuMI     PsiI    PspXI     PsrI     PvuI     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI
     XmaI     ZraI
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