Restriction map for pFN21AE0858
 (Linear) (Six-base) MAPSORT of: KIEE0858.seq  Check: 2509  from: 1  to: 945



KIEE0858.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     433     945 
   Size:        433     512
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     716     802     855     913     945 
   Size:        297     419      86      53      58      32

  Fragments arranged by size: 

                 419     297      86      58      53      32
 
AfeI AGC'GCT

Cuts at:      0     268     945 
   Size:        268     677
 
AhdI GACnn_n'nnGTC

Cuts at:      0     255     945 
   Size:        255     690
 
AleI CACnn'nnGTG

Cuts at:      0      22     742     945 
   Size:         22     720     203

  Fragments arranged by size: 

                 720     203      22
 
ApaLI G'TGCA_C

Cuts at:      0     751     945 
   Size:        751     194
 
ApoI r'AATT_y

Cuts at:      0      31     945 
   Size:         31     914
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     916     945 
   Size:        916      29
 
BbvCI CC'TCA_GC

Cuts at:      0     856     945 
   Size:        856      89
 
BglI GCCn_nnn'nGGC

Cuts at:      0     283     416     434     945 
   Size:        283     133      18     511

  Fragments arranged by size: 

                 511     283     133      18
 
Bme1580I G_kGCm'C

Cuts at:      0     195     755     945 
   Size:        195     560     190

  Fragments arranged by size: 

                 560     195     190
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     792     945 
   Size:        792     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     168     945 
   Size:         52     116     777

  Fragments arranged by size: 

                 777     116      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     856     945 
   Size:        856      89
 
BsaAI yAC'GTr

Cuts at:      0     369     540     945 
   Size:        369     171     405

  Fragments arranged by size: 

                 405     369     171
 
BsaBI GATnn'nnATC

Cuts at:      0     826     945 
   Size:        826     119
 
BsaWI w'CCGG_w

Cuts at:      0     804     945 
   Size:        804     141
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34     560     590     945 
   Size:          4      30     526      30     355

  Fragments arranged by size: 

                 526     355      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     628     637     945 
   Size:        628       9     308

  Fragments arranged by size: 

                 628     308       9
 
BseYI C'CCAG_C

Cuts at:      0     117     263     624     945 
   Size:        117     146     361     321

  Fragments arranged by size: 

                 361     321     146     117
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     945 
   Size:        405     540
 
BsiEI CG_ry'CG

Cuts at:      0     363     945 
   Size:        363     582
 
BsiHKAI G_wGCw'C

Cuts at:      0     404     755     945 
   Size:        404     351     190

  Fragments arranged by size: 

                 404     351     190
 
Bsp1286I G_dGCh'C

Cuts at:      0     195     404     755     945 
   Size:        195     209     351     190

  Fragments arranged by size: 

                 351     209     195     190
 
BspEI T'CCGG_A

Cuts at:      0     804     945 
   Size:        804     141
 
BspHI T'CATG_A

Cuts at:      0     404     945 
   Size:        404     541
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     260     433     945 
   Size:        134     126     173     512

  Fragments arranged by size: 

                 512     173     134     126
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     597     945 
   Size:        597     348
 
BstYI r'GATC_y

Cuts at:      0     209     518     683     945 
   Size:        209     309     165     262

  Fragments arranged by size: 

                 309     262     209     165
 
Bsu36I CC'TnA_GG

Cuts at:      0     877     945 
   Size:        877      68
 
BtgI C'CryG_G

Cuts at:      0     221     945 
   Size:        221     724
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     258     945 
   Size:        258     687
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     533     568     945 
   Size:        533      35     377

  Fragments arranged by size: 

                 533     377      35
 
EaeI y'GGCC_r

Cuts at:      0     137     224     245     371     482     587     668     945 
   Size:        137      87      21     126     111     105      81     277

  Fragments arranged by size: 

                 277     137     126     111     105      87      81      21
            
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     187     945 
   Size:         85     102     758

  Fragments arranged by size: 

                 758     102      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     168     297     716     802     855     913     945 
   Size:         52     116     129     419      86      53      58      32

  Fragments arranged by size: 

                 419     129     116      86      58      53      52      32
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     793     945 
   Size:        793     152
 
EcoRI G'AATT_C

Cuts at:      0      31     945 
   Size:         31     914
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     933     945 
   Size:        933      12
 
HaeII r_GCGC'y

Cuts at:      0     270     945 
   Size:        270     675
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     945 
   Size:        241      32     672

  Fragments arranged by size: 

                 672     241      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     682     945 
   Size:        682     263
 
MscI TGG'CCA

Cuts at:      0     139     226     373     589     945 
   Size:        139      87     147     216     356

  Fragments arranged by size: 

                 356     216     147     139      87
 
MslI CAynn'nnrTG

Cuts at:      0      22     172     349     466     742     820     945 
   Size:         22     150     177     117     276      78     125

  Fragments arranged by size: 

                 276     177     150     125     117      78      22
 
MspA1I CmG'CkG

Cuts at:      0      72     945 
   Size:         72     873
 
NspI r_CATG'y

Cuts at:      0     171     243     945 
   Size:        171      72     702

  Fragments arranged by size: 

                 702     171      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     146     945 
   Size:        146     799
 
PmlI CAC'GTG

Cuts at:      0     540     945 
   Size:        540     405
 
PpuMI rG'GwC_Cy

Cuts at:      0     793     945 
   Size:        793     152
 
PvuII CAG'CTG

Cuts at:      0      72     945 
   Size:         72     873
 
SapI GCTCTTCn'nnn_

Cuts at:      0      85     945 
   Size:         85     860
 
SphI G_CATG'C

Cuts at:      0     243     945 
   Size:        243     702
 
StuI AGG'CCT

Cuts at:      0     712     945 
   Size:        712     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     277     343     945 
   Size:        277      66     602

  Fragments arranged by size: 

                 602     277      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     560     592     945 
   Size:        560      32     353

  Fragments arranged by size: 

                 560     353      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279     945 
   Size:        279     666
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI     AleI    ApaLI     ApoI     BbsI
    BbvCI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NspI    PflMI     PmlI
    PpuMI    PvuII     SapI     SphI     StuI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AlfI
     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaHI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StyI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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