(Circular) (Six-base) MAPSORT of: pFN21AE0841.seq Check: 373 from: 1 to: 5731 pFN21AE0841 5731 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 3074 1937 Size: 885 252 4594 Fragments arranged by size: 4594 885 252 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 2331 3474 5046 2331 Size: 1143 1572 3016 Fragments arranged by size: 3016 1572 1143 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 2309 2451 2883 3040 3266 3418 5527 1493 Size: 816 142 432 157 226 152 2109 1697 Fragments arranged by size: 2109 1697 816 432 226 157 152 142 AseI AT'TA_AT Cuts at: 160 1032 2837 4285 160 Size: 872 1805 1448 1606 Fragments arranged by size: 1805 1606 1448 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2094 3056 1870 Size: 73 151 962 4545 Fragments arranged by size: 4545 962 151 73 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3052 4507 618 Size: 325 217 627 1265 1455 1842 Fragments arranged by size: 1842 1455 1265 627 325 217 BanII G_rGCy'C Cuts at: 1391 3050 1391 Size: 1659 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 4342 136 Size: 107 122 71 631 605 550 2120 1525 Fragments arranged by size: 2120 1525 631 605 550 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 3792 4948 1790 Size: 2002 1156 2573 Fragments arranged by size: 2573 2002 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3462 4420 442 Size: 416 53 83 399 24 2045 958 1753 Fragments arranged by size: 2045 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2552 4391 1239 Size: 1313 1839 2579 Fragments arranged by size: 2579 1839 1313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3856 4721 5019 3856 Size: 865 298 4568 Fragments arranged by size: 4568 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2487 3522 493 Size: 711 1283 1035 2702 Fragments arranged by size: 2702 1283 1035 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3538 3920 4555 275 Size: 53 83 186 936 2005 382 635 1451 Fragments arranged by size: 2005 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4164 4562 4836 4983 5394 4164 Size: 398 274 147 411 4501 Fragments arranged by size: 4501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5506 Fragments arranged by size: 5506 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5731 BseYI C'CCAG_C Cuts at: 2592 4934 5430 2592 Size: 2342 496 2893 Fragments arranged by size: 2893 2342 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2174 1163 Size: 573 318 120 4720 Fragments arranged by size: 4720 573 318 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2551 3792 3877 4948 2551 Size: 1241 85 1071 3334 Fragments arranged by size: 3334 1241 1071 85 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2551 3050 3792 3877 4948 1391 Size: 399 761 499 742 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 499 399 85 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3086 844 Size: 2242 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2211 4226 4400 65 Size: 1183 963 2015 174 1396 Fragments arranged by size: 2015 1396 1183 963 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 2106 96 Size: 2010 3721 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 3791 4803 3791 Size: 1012 4719 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 2814 1184 Size: 1630 4101 BstYI r'GATC_y Cuts at: 1972 2060 3061 3428 3813 3830 5282 5368 5380 Size: 88 1001 367 385 17 1452 86 12 Cuts at: 5380 5726 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 385 367 346 88 86 17 12 BstZ17I GTA'TAC Cuts at: 2823 2823 Size: 5731 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 3247 4041 4061 3247 Size: 794 20 4917 Fragments arranged by size: 4917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 2994 3035 2994 Size: 41 5690 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2748 3178 4067 Size: 54 1425 50 275 18 918 430 889 Cuts at: 4067 5696 8 Size: 1629 43 Fragments arranged by size: 1629 1425 918 889 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2359 3661 1353 Size: 972 34 1302 3423 Fragments arranged by size: 3423 1302 972 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2552 3794 4391 5178 5446 1239 Size: 393 920 1242 597 787 268 1524 Fragments arranged by size: 1524 1242 920 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2091 2091 Size: 5731 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 4878 1678 Size: 3200 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2464 2496 2920 2952 4372 Size: 32 135 32 892 32 424 32 1420 Cuts at: 4372 4404 4446 4478 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1420 892 424 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2222 2873 4845 5029 1305 Size: 917 651 1972 184 2007 Fragments arranged by size: 2007 1972 917 651 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 10 Size: 54 1425 343 918 2991 Fragments arranged by size: 2991 1425 918 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 3689 4048 4207 518 Size: 648 1296 1227 359 159 2042 Fragments arranged by size: 2042 1296 1227 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2188 2493 3147 3824 4972 5217 1606 Size: 582 305 654 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 654 582 305 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 3089 4634 1132 Size: 97 1860 1545 2229 Fragments arranged by size: 2229 1860 1545 97 PasI CC'CwG_GG Cuts at: 1348 2779 1348 Size: 1431 4300 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5731 PfoI T'CCnGG_A Cuts at: 2468 2468 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2091 Size: 5731 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 2736 2907 3301 2736 Size: 171 394 5166 Fragments arranged by size: 5166 394 171 PstI C_TGCA'G Cuts at: 830 3083 830 Size: 2253 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2188 2188 Size: 5731 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SanDI GG'GwC_CC Cuts at: 2091 2091 Size: 5731 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 3079 4214 4895 5086 826 Size: 219 2034 1135 681 191 1471 Fragments arranged by size: 2034 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2096 3058 1872 Size: 224 962 4545 Fragments arranged by size: 4545 962 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 3835 4736 4998 820 Size: 197 926 1892 901 262 1553 Fragments arranged by size: 1892 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 2702 152 Size: 2550 3181 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 2948 3170 513 Size: 552 1883 222 3074 Fragments arranged by size: 3074 1883 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2584 3758 3775 3928 4113 4558 325 Size: 1255 1004 1174 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1174 1004 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2315 3977 Size: 274 80 33 51 1443 129 209 1662 Cuts at: 3977 5689 96 Size: 1712 138 Fragments arranged by size: 1712 1662 1443 274 209 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5691 5723 5691 Size: 32 5699 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 2094 3056 1870 Size: 224 962 4545 Fragments arranged by size: 4545 962 224 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI