Restriction map for
pFN21AE0840
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 45 CGCG CG^CG
AseI 834 ATTAAT AT^TAAT
AsnI 834 ATTAAT AT^TAAT
AvaI 92 CCCGGG C^[CT]CG[GA]G
AvaII 59 GGTCC G^G[AT]CC
BalI 746 TGGCCA TGG^CCA
BfaI 671 CTAG C^TAG
BpmI 529 CTGGAG CTGGAG(16/14)
BsaBI 162 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 303 GAGGAG GAGGAG(10/8)
BsoBI 92 CCCGGG C^[CT]CG[GA]G
BspMI 325 ACCTGC ACCTGC(4/8)
BsrDI 393 CATTGC GCAATG(2/0)
BstEII 812 GGTTACC G^GT[ACGT]ACC
BstPI 812 GGTTACC G^GT[ACGT]ACC
BstUI 45 CGCG CG^CG
CfoI 227 GCGC GCG^C
Cfr13I 59 GGTCC G^G[ACGT]CC
DraI 990 TTTAAA TTT^AAA
DraII 58 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 406 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 946 CCATGG C^C[GA][CT]GG
EaeI 746 TGGCCA [CT]^GGCC[GA]
EarI 351 CTCTTC CTCTTC(1/4)
EcoO109I 58 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 812 GGTTACC G^GT[ACGT]ACC
EcoT14I 946 CCATGG C^C[AT][AT]GG
HhaI 227 GCGC GCG^C
HinP1I 227 GCGC G^CGC
HincII 597 GTTGAC GT[CT]^[GA]AC
HindII 597 GTTGAC GT[CT]^[GA]AC
Ksp632I 351 CTCTTC CTCTTC(1/4)
MaeI 671 CTAG C^TAG
MaeII 50 ACGT A^CGT
MamI 162 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 352 TCTTC GAAGA(8/7)
MluNI 746 TGGCCA TGG^CCA
MscI 746 TGGCCA TGG^CCA
MslI 456 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 45 CGCG CG^CG
NcoI 946 CCATGG C^CATGG
PpuMI 58 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 834 ATTAAT AT^TAAT
Sau96I 59 GGTCC G^G[ACGT]CC
SmaI 92 CCCGGG CCC^GGG
StyI 946 CCATGG C^C[AT][AT]GG
TaqI 383 TCGA T^CGA
TspRI 788 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 383 TCGA T^CGA
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 397 GCGG CCGC(-3/-1)
491 GCGG
AcsI 449 GAATTT [GA]^AATT[CT]
1014 GAATTT
ApoI 449 GAATTT [GA]^AATT[CT]
1014 GAATTT
BamHI 88 GGATCC G^GATCC
470 GGATCC
BglI 214 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
739 GCCAGAGTGGC
BsgI 187 CTGCAC GTGCAG(16/14)
31 GTGCAG
BsiYI 18 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
380 CCATCGACAGG
BslI 18 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
380 CCATCGACAGG
Bsp1407I 104 TGTACA T^GTACA
485 TGTACA
BsrGI 104 TGTACA T^GTACA
485 TGTACA
BstF5I 14 CATCC GGATG(2/0)
719 CATCC
BstYI 88 GGATCC [GA]^GATC[CT]
470 GGATCC
Cac8I 132 GCTGGC GC[ACGT]^[ACGT]GC
223 GCCTGC
DdeI 723 CTCAG C^T[ACGT]AG
909 CTCAG
DpnII 89 GATC ^GATC
471 GATC
FokI 14 CATCC GGATG(9/13)
719 CATCC
HaeIII 222 GGCC GG^CC
747 GGCC
HapII 85 CCGG C^CGG
93 CCGG
HpaII 85 CCGG C^CGG
93 CCGG
MboI 89 GATC ^GATC
471 GATC
MflI 88 GGATCC [GA]^GATC[CT]
470 GGATCC
MseI 835 TTAA T^TAA
991 TTAA
MspI 85 CCGG C^CGG
93 CCGG
NdeII 89 GATC ^GATC
471 GATC
PvuII 34 CAGCTG CAG^CTG
184 CAGCTG
Sau3AI 89 GATC ^GATC
471 GATC
SspBI 104 TGTACA T^GTACA
485 TGTACA
Tru9I 835 TTAA T^TAA
991 TTAA
Tsp45I 247 GTGAC ^GT[GC]AC
784 GTCAC
XhoII 88 GGATCC [GA]^GATC[CT]
470 GGATCC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI AlwI BbvI BcnI
BsaJI BsrI BssKI BstNI EcoRII
Fnu4HI HinfI HphI ItaI MaeIII
MnlI MspA1I MvaI MwoI NciI
NlaIII NlaIV RsaI ScrFI SfaNI
TfiI TseI Tsp509I