Restriction map for pFN21AE0840
 (Circular) (Six-base) MAPSORT of: pFN21AE0840.seq  Check: 382  from: 1  to: 5740



pFN21AE0840  5740 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2339    2339 
   Size:       5740
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3550     278 
   Size:         53      83     186     936    2014    2468

  Fragments arranged by size: 

                2468    2014     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3083    1937 
   Size:       1146    4594
 
Acc65I G'GTAC_C

Cuts at:   3061    3061 
   Size:       5740
 
AclI AA'CG_TT

Cuts at:   1563    3867    4240    1563 
   Size:       2304     373    3063

  Fragments arranged by size: 

                3063    2304     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2251    3803    5187    5455    1632 
   Size:        619    1552    1384     268    1917

  Fragments arranged by size: 

                1917    1552    1384     619     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5543
 
AflIII A'CryG_T

Cuts at:   1203    4639    5616    1203 
   Size:       3436     977    1327

  Fragments arranged by size: 

                3436    1327     977
 
AgeI A'CCGG_T

Cuts at:   4571    4571 
   Size:       5740
 
AhdI GACnn_n'nnGTC

Cuts at:   4469    4469 
   Size:       5740
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5740
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5740
 
AlwNI CAG_nnn'CTG

Cuts at:   3483    5055    3483 
   Size:       1572    4168
 
ApaLI G'TGCA_C

Cuts at:   3797    4953    3797 
   Size:       1156    4584
 
ApoI r'AATT_y

Cuts at:   1493    2454    3019    3049    3275    3427    5536    1493 
   Size:        961     565      30     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     961     565     226     152      30
 
AseI AT'TA_AT

Cuts at:    160    1032    2840    4294     160 
   Size:        872    1808    1454    1606

  Fragments arranged by size: 

                1808    1606    1454     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5740
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2097    3065    1870 
   Size:         73     154     968    4545

  Fragments arranged by size: 

                4545     968     154      73
 
BamHI G'GATC_C

Cuts at:   2093    2475    3070    3437    2093 
   Size:        382     595     367    4396

  Fragments arranged by size: 

                4396     595     382     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3061    4516     618 
   Size:        325     217     627    1274    1455    1842

  Fragments arranged by size: 

                1842    1455    1274     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3059    1391 
   Size:       1668    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4769
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3931    3965    1903 
   Size:         34    1994      34    3678

  Fragments arranged by size: 

                3678    1994      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3632    4842    3632 
   Size:       1210    4530
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5740
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2225    2750    4351 
   Size:        107     122      71     631     605     553     525    1601

Cuts at:   4351     136 
   Size:       1525

  Fragments arranged by size: 

                1601    1525     631     605     553     525     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5735    5735 
   Size:       5740
 
BlpI GC'TnA_GC

Cuts at:   3157    3157 
   Size:       5740
 
Bme1580I G_kGCm'C

Cuts at:   1790    3801    4957    1790 
   Size:       2011    1156    2573

  Fragments arranged by size: 

                2573    2011    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5740
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2605    3471    4429 
   Size:        416      53      83     399      24    1188     866     958

Cuts at:   4429     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1188     958     866     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5740
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2555    4400    1239 
   Size:       1316    1845    2579

  Fragments arranged by size: 

                2579    1845    1316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3865    4730    5028    3865 
   Size:        865     298    4577

  Fragments arranged by size: 

                4577     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4403     882 
   Size:        617    2904    2219

  Fragments arranged by size: 

                2904    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3531     493 
   Size:        711    2327    2702

  Fragments arranged by size: 

                2702    2327     711
 
BsaBI GATnn'nnATC

Cuts at:   2171    5734    2171 
   Size:       3563    2177
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3547    3929    4564     275 
   Size:         53      83     186     936    2014     382     635    1451

  Fragments arranged by size: 

                2014    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4173    4571    4845    4992    5403    4173 
   Size:        398     274     147     411    4510

  Fragments arranged by size: 

                4510     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2302    2332    1178 
   Size:         30     165      30     899      30    4586

  Fragments arranged by size: 

                4586     899     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2323    1186 
   Size:       1137    4603
 
BseYI C'CCAG_C

Cuts at:   4943    5439    4943 
   Size:        496    5244
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2057    2177    1163 
   Size:        573     321     120    4726

  Fragments arranged by size: 

                4726     573     321     120
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3190    3951    4979    1540 
   Size:        275     186    1189     761    1028    2301

  Fragments arranged by size: 

                2301    1189    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3801    3886    4957    3801 
   Size:         85    1071    4584

  Fragments arranged by size: 

                4584    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3251    3344    3251 
   Size:         93    5647
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3059    3801    3886    4957    1391 
   Size:        399    1269     742      85    1071    2174

  Fragments arranged by size: 

                2174    1269    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5403    5403 
   Size:       5740
 
BspHI T'CATG_A

Cuts at:   3629    5359    3629 
   Size:       1730    4010
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2339    3095     844 
   Size:       1495     756    3489

  Fragments arranged by size: 

                3489    1495     756
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3627    1394 
   Size:        429    1804    3507

  Fragments arranged by size: 

                3507    1804     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2397    4235    4409      65 
   Size:       1183    1149    1838     174    1396

  Fragments arranged by size: 

                1838    1396    1183    1149     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4384    4571    1700 
   Size:        110    2574     187    2869

  Fragments arranged by size: 

                2869    2574     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2109    2490      96 
   Size:       2013     381    3346

  Fragments arranged by size: 

                3346    2013     381
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5740
 
BssSI C'ACGA_G

Cuts at:   3800    4812    3800 
   Size:       1012    4728
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5740
 
BstBI TT'CG_AA

Cuts at:   4544    4544 
   Size:       5740
 
BstEII G'GTnAC_C

Cuts at:   1184    2817    1184 
   Size:       1633    4107
 
BstYI r'GATC_y

Cuts at:   1972    2093    2475    3070    3437    3822    3839    5291    5377 
   Size:        121     382     595     367     385      17    1452      86

Cuts at:   5377    5389    5735    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     595     385     382     367     346     121
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2951     513 
   Size:        552    1886    3302

  Fragments arranged by size: 

                3302    1886     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5001

  Fragments arranged by size: 

                5001     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3256    4050    4070    3256 
   Size:        794      20    4926

  Fragments arranged by size: 

                4926     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5740
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5705
 
DraI TTT'AAA

Cuts at:   2997    3044    2997 
   Size:         47    5693
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2417    4747     809 
   Size:        507    1101    2330    1802

  Fragments arranged by size: 

                2330    1802    1101     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2751    3187    4076 
   Size:         54    1425      50     275      18     921     436     889

Cuts at:   4076    5705       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     921     889     436     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3187    1537 
   Size:        275    1375    4090

  Fragments arranged by size: 

                4090    1375     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2362    3670    1353 
   Size:       1009    1308    3423

  Fragments arranged by size: 

                3423    1308    1009
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4327    4701    4847     387 
   Size:       1064     138    2738     374     146    1280

  Fragments arranged by size: 

                2738    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2251    2555    3803    4400    5187    5455    1239 
   Size:        393     619     304    1248     597     787     268    1524

  Fragments arranged by size: 

                1524    1248     787     619     597     393     304     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2064    2064 
   Size:       5740
 
EcoRI G'AATT_C

Cuts at:   3049    3427    3049 
   Size:        378    5362
 
FspI TGC'GCA

Cuts at:   4246    4246 
   Size:       5740
 
HaeII r_GCGC'y

Cuts at:   1678    4887    1678 
   Size:       3209    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2528    2560    2600    2632    4381 
   Size:         32     135      32     956      32      40      32    1749

Cuts at:   4381    4413    4455    4487    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1749     956     135      42      40      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2604    3084    3330     669 
   Size:        988     281     666     480     246    3079

  Fragments arranged by size: 

                3079     988     666     480     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5740
 
HpaI GTT'AAC

Cuts at:   3330    3330 
   Size:       5740
 
KpnI G_GTAC'C

Cuts at:   3065    3065 
   Size:       5740
 
MfeI C'AATT_G

Cuts at:   3339    3339 
   Size:       5740
 
MluI A'CGCG_T

Cuts at:   5616    5616 
   Size:       5740
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2225    4854    5038    1305 
   Size:        920    2629     184    2007

  Fragments arranged by size: 

                2629    2007     920     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2753      10 
   Size:         54    1425     343     921    2997

  Fragments arranged by size: 

                2997    1425     921     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2465    3698    4057    4216     518 
   Size:        648    1299    1233     359     159    2042

  Fragments arranged by size: 

                2042    1299    1233     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2041    2191    2496    3156    3833    4981    5226    1606 
   Size:        435     150     305     660     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     660     435     305     245     150
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2951     513 
   Size:        552    1886    3302

  Fragments arranged by size: 

                3302    1886     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5740
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5740
 
NotI GC'GGCC_GC

Cuts at:   3187    3187 
   Size:       5740
 
NspI r_CATG'y

Cuts at:   1132    1229    3098    4643    1132 
   Size:         97    1869    1545    2229

  Fragments arranged by size: 

                2229    1869    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2782    1348 
   Size:       1434    4306
 
PciI A'CATG_T

Cuts at:   4639    4639 
   Size:       5740
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5740
 
PfoI T'CCnGG_A

Cuts at:   2471    2471 
   Size:       5740
 
PmeI GTTT'AAAC

Cuts at:   3044    3044 
   Size:       5740
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3809    3841    5322    5354    3809 
   Size:         32    1481      32    4195

  Fragments arranged by size: 

                4195    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2064    2064 
   Size:       5740
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5740
 
PsiI TTA'TAA

Cuts at:   2864    2910    3310    2864 
   Size:         46     400    5294

  Fragments arranged by size: 

                5294     400      46
 
PstI C_TGCA'G

Cuts at:    830    3092     830 
   Size:       2262    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5740
 
PvuII CAG'CTG

Cuts at:   2041    2191    2041 
   Size:        150    5590
 
SalI G'TCGA_C

Cuts at:   1936    3082    1936 
   Size:       1146    4594
 
SbfI CC_TGCA'GG

Cuts at:   3092    3092 
   Size:       5740
 
ScaI AGT'ACT

Cuts at:   3988    5700    3988 
   Size:       1712    4028
 
SfcI C'TryA_G

Cuts at:    826    1045    3088    4223    4904    5095     826 
   Size:        219    2043    1135     681     191    1471

  Fragments arranged by size: 

                2043    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5740
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5740
 
SmaI CCC'GGG

Cuts at:   1872    2099    3067    1872 
   Size:        227     968    4545

  Fragments arranged by size: 

                4545     968     227
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3844    4745    5007     820 
   Size:        197     926    1901     901     262    1553

  Fragments arranged by size: 

                1901    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3531     493 
   Size:       3038    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5740
 
SphI G_CATG'C

Cuts at:   1132    3098    1132 
   Size:       1966    3774
 
SspI AAT'ATT

Cuts at:      5      52    3664       5 
   Size:         47    3612    2081

  Fragments arranged by size: 

                3612    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5740
 
StyI C'CwwG_G

Cuts at:    513    1065    2951    3179     513 
   Size:        552    1886     228    3074

  Fragments arranged by size: 

                3074    1886     552     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2237    2587    3767    3784    3937    4122    4567 
   Size:       1255     657     350    1180      17     153     185     445

Cuts at:   4567     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1180     657     445     350     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2109    2318    2490 
   Size:        274      80      33      51    1443     132     209     172

Cuts at:   2490    3986    5698      96 
   Size:       1496    1712     138

  Fragments arranged by size: 

                1712    1496    1443     274     209     172     138     132
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2085    2117    5700    5732    2085 
   Size:         32    3583      32    2093

  Fragments arranged by size: 

                3583    2093      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5740
 
XbaI T'CTAG_A

Cuts at:   3076    3076 
   Size:       5740
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5740
 
XmaI C'CCGG_G

Cuts at:   1870    2097    3065    1870 
   Size:        227     968    4545

  Fragments arranged by size: 

                4545     968     227
 
XmnI GAAnn'nnTTC

Cuts at:   3869    3869 
   Size:       5740
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3548     276 
   Size:         53      83     186     936    2014    2468

  Fragments arranged by size: 

                2468    2014     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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