(Circular) (Six-base) MAPSORT of: pFN21AE0840.seq Check: 382 from: 1 to: 5740 pFN21AE0840 5740 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2339 2339 Size: 5740 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2251 3803 5187 5455 1632 Size: 619 1552 1384 268 1917 Fragments arranged by size: 1917 1552 1384 619 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 2454 3019 3049 3275 3427 5536 1493 Size: 961 565 30 226 152 2109 1697 Fragments arranged by size: 2109 1697 961 565 226 152 30 AseI AT'TA_AT Cuts at: 160 1032 2840 4294 160 Size: 872 1808 1454 1606 Fragments arranged by size: 1808 1606 1454 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2097 3065 1870 Size: 73 154 968 4545 Fragments arranged by size: 4545 968 154 73 BamHI G'GATC_C Cuts at: 2093 2475 3070 3437 2093 Size: 382 595 367 4396 Fragments arranged by size: 4396 595 382 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 3059 1391 Size: 1668 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2225 2750 4351 Size: 107 122 71 631 605 553 525 1601 Cuts at: 4351 136 Size: 1525 Fragments arranged by size: 1601 1525 631 605 553 525 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 3801 4957 1790 Size: 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2605 3471 4429 Size: 416 53 83 399 24 1188 866 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1188 958 866 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 4400 1239 Size: 1316 1845 2579 Fragments arranged by size: 2579 1845 1316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4403 882 Size: 617 2904 2219 Fragments arranged by size: 2904 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 2171 5734 2171 Size: 3563 2177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2302 2332 1178 Size: 30 165 30 899 30 4586 Fragments arranged by size: 4586 899 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2323 1186 Size: 1137 4603 BseYI C'CCAG_C Cuts at: 4943 5439 4943 Size: 496 5244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2177 1163 Size: 573 321 120 4726 Fragments arranged by size: 4726 573 321 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3801 3886 4957 3801 Size: 85 1071 4584 Fragments arranged by size: 4584 1071 85 BsmI GAATG_Cn' Cuts at: 3251 3344 3251 Size: 93 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3059 3801 3886 4957 1391 Size: 399 1269 742 85 1071 2174 Fragments arranged by size: 2174 1269 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2339 3095 844 Size: 1495 756 3489 Fragments arranged by size: 3489 1495 756 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 4235 4409 65 Size: 1183 1149 1838 174 1396 Fragments arranged by size: 1838 1396 1183 1149 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 2109 2490 96 Size: 2013 381 3346 Fragments arranged by size: 3346 2013 381 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 2817 1184 Size: 1633 4107 BstYI r'GATC_y Cuts at: 1972 2093 2475 3070 3437 3822 3839 5291 5377 Size: 121 382 595 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 595 385 382 367 346 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2951 513 Size: 552 1886 3302 Fragments arranged by size: 3302 1886 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 2997 3044 2997 Size: 47 5693 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2417 4747 809 Size: 507 1101 2330 1802 Fragments arranged by size: 2330 1802 1101 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2751 3187 4076 Size: 54 1425 50 275 18 921 436 889 Cuts at: 4076 5705 8 Size: 1629 43 Fragments arranged by size: 1629 1425 921 889 436 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2362 3670 1353 Size: 1009 1308 3423 Fragments arranged by size: 3423 1308 1009 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2251 2555 3803 4400 5187 5455 1239 Size: 393 619 304 1248 597 787 268 1524 Fragments arranged by size: 1524 1248 787 619 597 393 304 268 EcoO109I rG'GnC_Cy Cuts at: 2064 2064 Size: 5740 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 2600 2632 4381 Size: 32 135 32 956 32 40 32 1749 Cuts at: 4381 4413 4455 4487 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1749 956 135 42 40 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2604 3084 3330 669 Size: 988 281 666 480 246 3079 Fragments arranged by size: 3079 988 666 480 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2225 4854 5038 1305 Size: 920 2629 184 2007 Fragments arranged by size: 2629 2007 920 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2753 10 Size: 54 1425 343 921 2997 Fragments arranged by size: 2997 1425 921 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 3698 4057 4216 518 Size: 648 1299 1233 359 159 2042 Fragments arranged by size: 2042 1299 1233 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2191 2496 3156 3833 4981 5226 1606 Size: 435 150 305 660 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 435 305 245 150 NcoI C'CATG_G Cuts at: 513 1065 2951 513 Size: 552 1886 3302 Fragments arranged by size: 3302 1886 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 2782 1348 Size: 1434 4306 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PfoI T'CCnGG_A Cuts at: 2471 2471 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 2064 Size: 5740 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 2864 2910 3310 2864 Size: 46 400 5294 Fragments arranged by size: 5294 400 46 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2041 2191 2041 Size: 150 5590 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 3088 4223 4904 5095 826 Size: 219 2043 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 2099 3067 1872 Size: 227 968 4545 Fragments arranged by size: 4545 968 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2951 3179 513 Size: 552 1886 228 3074 Fragments arranged by size: 3074 1886 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 2587 3767 3784 3937 4122 4567 Size: 1255 657 350 1180 17 153 185 445 Cuts at: 4567 325 Size: 1498 Fragments arranged by size: 1498 1255 1180 657 445 350 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2318 2490 Size: 274 80 33 51 1443 132 209 172 Cuts at: 2490 3986 5698 96 Size: 1496 1712 138 Fragments arranged by size: 1712 1496 1443 274 209 172 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 5700 5732 2085 Size: 32 3583 32 2093 Fragments arranged by size: 3583 2093 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 2097 3065 1870 Size: 227 968 4545 Fragments arranged by size: 4545 968 227 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI