Restriction map for pFN21AE0830
 (Circular) (Six-base) MAPSORT of: pFN21AE0830.seq  Check: 2890  from: 1  to: 5464



pFN21AE0830  5464 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2226    2226 
   Size:       5464
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3274     278 
   Size:         53      83     186     936    1738    2468

  Fragments arranged by size: 

                2468    1738     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2807    1937 
   Size:        870    4594
 
Acc65I G'GTAC_C

Cuts at:   2785    2785 
   Size:       5464
 
AclI AA'CG_TT

Cuts at:   1563    3591    3964    1563 
   Size:       2028     373    3063

  Fragments arranged by size: 

                3063    2028     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2405    3527    4911    5179    1632 
   Size:        773    1122    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1122     773     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5267
 
AflIII A'CryG_T

Cuts at:   1203    4363    5340    1203 
   Size:       3160     977    1327

  Fragments arranged by size: 

                3160    1327     977
 
AgeI A'CCGG_T

Cuts at:   4295    4295 
   Size:       5464
 
AhdI GACnn_n'nnGTC

Cuts at:   4193    4193 
   Size:       5464
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5464
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2234    1184 
   Size:       1050    4414
 
AlwNI CAG_nnn'CTG

Cuts at:   3207    4779    3207 
   Size:       1572    3892
 
ApaLI G'TGCA_C

Cuts at:   3521    4677    3521 
   Size:       1156    4308
 
ApoI r'AATT_y

Cuts at:   1493    2773    2999    3151    5260    1493 
   Size:       1280     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1280     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4018     160 
   Size:        872    2986    1606

  Fragments arranged by size: 

                2986    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5464
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2789    1870 
   Size:         73     846    4545

  Fragments arranged by size: 

                4545     846      73
 
BamHI G'GATC_C

Cuts at:   2794    3161    2794 
   Size:        367    5097
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2785    4240     618 
   Size:        325     217     627     998    1455    1842

  Fragments arranged by size: 

                1842    1455     998     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2783    1391 
   Size:       1392    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2672     928 
   Size:        971     773    3720

  Fragments arranged by size: 

                3720     971     773
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3655    3689    1903 
   Size:         34    1718      34    3678

  Fragments arranged by size: 

                3678    1718      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2385    3356    4566    2385 
   Size:        971    1210    3283

  Fragments arranged by size: 

                3283    1210     971
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5464
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4075     136 
   Size:        107     122      71     631     605    2403    1525

  Fragments arranged by size: 

                2403    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2038    2163    5459    2038 
   Size:        125    3296    2043

  Fragments arranged by size: 

                3296    2043     125
 
BlpI GC'TnA_GC

Cuts at:   2881    2881 
   Size:       5464
 
Bme1580I G_kGCm'C

Cuts at:   1790    3525    4681    1790 
   Size:       1735    1156    2573

  Fragments arranged by size: 

                2573    1735    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5464
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2289    3195    4153 
   Size:        416      53      83     399      24     872     906     958

Cuts at:   4153     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     906     872     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5464
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4124    1239 
   Size:       2885    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3589    4454    4752    3589 
   Size:        865     298    4301

  Fragments arranged by size: 

                4301     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4127     882 
   Size:        617    2628    2219

  Fragments arranged by size: 

                2628    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3255     493 
   Size:        711    2051    2702

  Fragments arranged by size: 

                2702    2051     711
 
BsaBI GATnn'nnATC

Cuts at:   5458    5458 
   Size:       5464
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3271    3653    4288     275 
   Size:         53      83     186     936    1738     382     635    1451

  Fragments arranged by size: 

                1738    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3897    4295    4569    4716    5127    3897 
   Size:        398     274     147     411    4234

  Fragments arranged by size: 

                4234     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5239

  Fragments arranged by size: 

                5239     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5464
 
BseYI C'CCAG_C

Cuts at:   4667    5163    4667 
   Size:        496    4968
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2204    1163 
   Size:        573     468    4423

  Fragments arranged by size: 

                4423     573     468
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2914    3675    4703    1540 
   Size:        275     186     913     761    1028    2301

  Fragments arranged by size: 

                2301    1028     913     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3525    3610    4681    3525 
   Size:         85    1071    4308

  Fragments arranged by size: 

                4308    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2975    3068    2975 
   Size:         93    5371
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2783    3525    3610    4681    1391 
   Size:        399     993     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     993     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5127    5127 
   Size:       5464
 
BspHI T'CATG_A

Cuts at:   3353    5083    3353 
   Size:       1730    3734
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2226    2819     844 
   Size:       1382     593    3489

  Fragments arranged by size: 

                3489    1382     593
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3351    1394 
   Size:        429    1528    3507

  Fragments arranged by size: 

                3507    1528     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2187    2661    3959    4133      65 
   Size:       1183     939     474    1298     174    1396

  Fragments arranged by size: 

                1396    1298    1183     939     474     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4108    4295    1700 
   Size:        110    2298     187    2869

  Fragments arranged by size: 

                2869    2298     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5464
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5464
 
BssSI C'ACGA_G

Cuts at:   3524    4536    3524 
   Size:       1012    4452
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5464
 
BstBI TT'CG_AA

Cuts at:   4268    4268 
   Size:       5464
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5464
 
BstYI r'GATC_y

Cuts at:   1972    2038    2163    2794    3161    3546    3563    5015    5101 
   Size:         66     125     631     367     385      17    1452      86

Cuts at:   5101    5113    5459    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     631     385     367     346     125      86
                  66      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4912
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4725

  Fragments arranged by size: 

                4725     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2980    3774    3794    2980 
   Size:        794      20    4650

  Fragments arranged by size: 

                4650     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5464
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5429
 
DraI TTT'AAA

Cuts at:   2768    2768 
   Size:       5464
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4471     809 
   Size:        507    3155    1802

  Fragments arranged by size: 

                3155    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2911    3800    5429 
   Size:         54    1425      50     275      18    1081     889    1629

Cuts at:   5429       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1081     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2911    1537 
   Size:        275    1099    4090

  Fragments arranged by size: 

                4090    1099     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2600    2654    3394    1353 
   Size:       1247      54     740    3423

  Fragments arranged by size: 

                3423    1247     740      54
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4051    4425    4571     387 
   Size:       1064     138    2462     374     146    1280

  Fragments arranged by size: 

                2462    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2405    3527    4124    4911    5179    1239 
   Size:        393     773    1122     597     787     268    1524

  Fragments arranged by size: 

                1524    1122     787     773     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2773    3151    2773 
   Size:        378    5086
 
FspI TGC'GCA

Cuts at:   3970    3970 
   Size:       5464
 
HaeII r_GCGC'y

Cuts at:   1678    4611    1678 
   Size:       2933    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4105    4137    4179    4211    1373 
   Size:         32     135      32    2533      32      42      32    2626

  Fragments arranged by size: 

                2626    2533     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2808    3054     669 
   Size:        988     281     870     246    3079

  Fragments arranged by size: 

                3079     988     870     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5464
 
HpaI GTT'AAC

Cuts at:   3054    3054 
   Size:       5464
 
KpnI G_GTAC'C

Cuts at:   2789    2789 
   Size:       5464
 
MfeI C'AATT_G

Cuts at:   2744    3063    2744 
   Size:        319    5145
 
MluI A'CGCG_T

Cuts at:   5340    5340 
   Size:       5464
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4578    4762    1305 
   Size:       3273     184    2007

  Fragments arranged by size: 

                3273    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3642

  Fragments arranged by size: 

                3642    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3422    3781    3940     518 
   Size:        648    2256     359     159    2042

  Fragments arranged by size: 

                2256    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2880    3557    4705    4950    1606 
   Size:       1274     677    1148     245    2120

  Fragments arranged by size: 

                2120    1274    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4912
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5464
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5464
 
NotI GC'GGCC_GC

Cuts at:   2911    2911 
   Size:       5464
 
NspI r_CATG'y

Cuts at:   1132    1229    2695    2822    4367    1132 
   Size:         97    1466     127    1545    2229

  Fragments arranged by size: 

                2229    1545    1466     127      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5464
 
PciI A'CATG_T

Cuts at:   4363    4363 
   Size:       5464
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5464
 
PmeI GTTT'AAAC

Cuts at:   2768    2768 
   Size:       5464
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3533    3565    5046    5078    3533 
   Size:         32    1481      32    3919

  Fragments arranged by size: 

                3919    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5464
 
PsiI TTA'TAA

Cuts at:   3034    3034 
   Size:       5464
 
PstI C_TGCA'G

Cuts at:    830    2816     830 
   Size:       1986    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5464
 
SalI G'TCGA_C

Cuts at:   1936    2806    1936 
   Size:        870    4594
 
SbfI CC_TGCA'GG

Cuts at:   2816    2816 
   Size:       5464
 
ScaI AGT'ACT

Cuts at:   2360    3712    5424    2360 
   Size:       1352    1712    2400

  Fragments arranged by size: 

                2400    1712    1352
 
SfcI C'TryA_G

Cuts at:    826    1045    2548    2812    3947    4628    4819     826 
   Size:        219    1503     264    1135     681     191    1471

  Fragments arranged by size: 

                1503    1471    1135     681     264     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5464
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5464
 
SmaI CCC'GGG

Cuts at:   1872    2791    1872 
   Size:        919    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3568    4469    4731     820 
   Size:        197     926    1625     901     262    1553

  Fragments arranged by size: 

                1625    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3255     493 
   Size:       2762    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5464
 
SphI G_CATG'C

Cuts at:   1132    2822    1132 
   Size:       1690    3774
 
SspI AAT'ATT

Cuts at:      5      52    3388       5 
   Size:         47    3336    2081

  Fragments arranged by size: 

                3336    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2442    1517 
   Size:        925    4539
 
StyI C'CwwG_G

Cuts at:    513    1065    2149    2903     513 
   Size:        552    1084     754    3074

  Fragments arranged by size: 

                3074    1084     754     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3491    3508    3661    3846    4291     325 
   Size:       1255    1911      17     153     185     445    1498

  Fragments arranged by size: 

                1911    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2276    2358    3710 
   Size:        274      80      33      51    1443     299      82    1352

Cuts at:   3710    5422      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1352     299     274     138      82      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5424    5456    5424 
   Size:         32    5432
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5464
 
XbaI T'CTAG_A

Cuts at:   2800    2800 
   Size:       5464
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5464
 
XmaI C'CCGG_G

Cuts at:   1870    2789    1870 
   Size:        919    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3593    3593 
   Size:       5464
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3272     276 
   Size:         53      83     186     936    1738    2468

  Fragments arranged by size: 

                2468    1738     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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