Restriction map for
pFN21AE0827
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 420 GTCTAC GT^[AC][GT]AC
Alw44I 273 GTGCAC G^TGCAC
ApaLI 273 GTGCAC G^TGCAC
AspHI 273 GTGCAC G[AT]GC[AT]^C
BbsI 203 GTCTTC GAAGAC(2/6)
BbvI 428 GCAGC GCAGC(8/12)
BglII 41 AGATCT A^GATCT
BmyI 273 GTGCAC G[AGT]GC[ACT]^C
BpmI 158 CTGGAG CTGGAG(16/14)
BpuAI 203 GTCTTC GAAGAC(2/6)
BsaAI 76 TACGTG [CT]AC^GT[GA]
BsaJI 443 CCGTGG C^C[ACGT][ACGT]GG
BseRI 652 CTCCTC GAGGAG(10/8)
BsgI 97 CTGCAC GTGCAG(16/14)
BsiHKAI 273 GTGCAC G[AT]GC[AT]^C
BsmAI 493 GTCTC GTCTC(1/5)
BsmFI 648 GTCCC GGGAC(10/14)
Bsp1286I 273 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 197 TGTACA T^GTACA
BspHI 113 TCATGA T^CATGA
BspLU11I 248 ACATGT A^CATGT
BspMI 424 ACCTGC ACCTGC(4/8)
BsrDI 322 GCAATG GCAATG(2/0)
BsrGI 197 TGTACA T^GTACA
BstF5I 132 CATCC GGATG(2/0)
BstXI 21 CCACTTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 41 AGATCT [GA]^GATC[CT]
DdeI 38 CTGAG C^T[ACGT]AG
DsaI 443 CCGTGG C^C[GA][CT]GG
Fnu4HI 428 GCAGC GC^[ACGT]GC
FokI 132 CATCC GGATG(9/13)
ItaI 428 GCAGC GC^[ACGT]GC
MflI 41 AGATCT [GA]^GATC[CT]
NlaIV 217 GGCCCC GG[ACGT]^[ACGT]CC
NspI 248 ACATGT [GA]CATG^[CT]
PleI 365 GAGTC GAGTC(4/5)
PstI 426 CTGCAG CTGCA^G
RcaI 113 TCATGA T^CATGA
SspBI 197 TGTACA T^GTACA
StuI 294 AGGCCT AGG^CCT
TaqI 120 TCGA T^CGA
TseI 428 GCAGC G^C[AT]GC
Tsp45I 245 GTGAC ^GT[GC]AC
TthHB8I 120 TCGA T^CGA
XhoII 41 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 514 AAATTC [GA]^AATT[CT]
579 AAATTC
AfaI 198 GTAC GT^AC
252 GTAC
AflIII 248 ACATGT A^C[GA][CT]GT
569 ACACGT
ApoI 514 AAATTC [GA]^AATT[CT]
579 AAATTC
BalI 80 TGGCCA TGG^CCA
149 TGGCCA
BsrI 157 ACTGG ACTGG(1/-1)
267 ACTGG
Cfr13I 3 GGGCC G^G[ACGT]CC
217 GGCCC
DpnII 42 GATC ^GATC
70 GATC
EaeI 80 TGGCCA [CT]^GGCC[GA]
149 TGGCCA
HphI 57 GGTGA GGTGA(8/7)
440 TCACC
MaeII 77 ACGT A^CGT
571 ACGT
MaeIII 245 GTGAC ^GT[ACGT]AC
520 GTTAC
MboI 42 GATC ^GATC
70 GATC
MluNI 80 TGGCCA TGG^CCA
149 TGGCCA
MscI 80 TGGCCA TGG^CCA
149 TGGCCA
MseI 106 TTAA T^TAA
383 TTAA
MslI 438 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
543 CACACCCATG
NdeII 42 GATC ^GATC
70 GATC
RsaI 198 GTAC GT^AC
252 GTAC
Sau3AI 42 GATC ^GATC
70 GATC
Sau96I 3 GGGCC G^G[ACGT]CC
217 GGCCC
TfiI 117 GAATC G^A[AT]TC
555 GAATC
Tru9I 106 TTAA T^TAA
383 TTAA
XcmI 21 CCACTTGTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
131 CCATCCTTCTGTTGG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AluI BsiYI BslI Cac8I HaeIII
HinfI MboII MnlI NlaIII SfcI
Tsp509I TspRI