Restriction map for
pFN21AE0821
(Linear) (Six-base) MAPSORT of: KIEE0821.seq Check: 1185 from: 1 to: 810
KIEE0821.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 810
Size: 541 269
AleI CACnn'nnGTG
Cuts at: 0 262 810
Size: 262 548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 810
Size: 191 32 587
Fragments arranged by size:
587 191 32
BanII G_rGCy'C
Cuts at: 0 177 810
Size: 177 633
BbsI GAAGACnn'nnnn_
Cuts at: 0 4 207 810
Size: 4 203 603
Fragments arranged by size:
603 203 4
BglI GCCn_nnn'nGGC
Cuts at: 0 358 592 810
Size: 358 234 218
Fragments arranged by size:
358 234 218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 792 810
Size: 792 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 682 810
Size: 124 558 128
Fragments arranged by size:
558 128 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 810
Size: 441 369
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 810
Size: 191 30 589
Fragments arranged by size:
589 191 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 768 810
Size: 768 42
BseYI C'CCAG_C
Cuts at: 0 298 731 810
Size: 298 433 79
Fragments arranged by size:
433 298 79
BsiHKAI G_wGCw'C
Cuts at: 0 575 810
Size: 575 235
BsmI GAATG_Cn'
Cuts at: 0 667 810
Size: 667 143
Bsp1286I G_dGCh'C
Cuts at: 0 177 575 810
Size: 177 398 235
Fragments arranged by size:
398 235 177
BsrGI T'GTAC_A
Cuts at: 0 146 810
Size: 146 664
Bsu36I CC'TnA_GG
Cuts at: 0 269 810
Size: 269 541
BtgI C'CryG_G
Cuts at: 0 383 810
Size: 383 427
BtsI GCAGTG_nn'
Cuts at: 0 152 810
Size: 152 658
DrdI GACnn_nn'nnGTC
Cuts at: 0 610 810
Size: 610 200
EaeI y'GGCC_r
Cuts at: 0 380 810
Size: 380 430
EarI CTCTTCn'nnn_
Cuts at: 0 517 786 810
Size: 517 269 24
Fragments arranged by size:
517 269 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 541 682 810
Size: 124 417 141 128
Fragments arranged by size:
417 141 128 124
EcoRV GAT'ATC
Cuts at: 0 243 810
Size: 243 567
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 164 196 810
Size: 164 32 614
Fragments arranged by size:
614 164 32
MfeI C'AATT_G
Cuts at: 0 589 810
Size: 589 221
MscI TGG'CCA
Cuts at: 0 382 810
Size: 382 428
MslI CAynn'nnrTG
Cuts at: 0 262 580 810
Size: 262 318 230
Fragments arranged by size:
318 262 230
NcoI C'CATG_G
Cuts at: 0 383 810
Size: 383 427
NspI r_CATG'y
Cuts at: 0 42 810
Size: 42 768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 191 223 810
Size: 191 32 587
Fragments arranged by size:
587 191 32
PstI C_TGCA'G
Cuts at: 0 159 810
Size: 159 651
SfcI C'TryA_G
Cuts at: 0 155 810
Size: 155 655
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 592 810
Size: 592 218
SmlI C'TyrA_G
Cuts at: 0 420 810
Size: 420 390
StuI AGG'CCT
Cuts at: 0 274 810
Size: 274 536
StyI C'CwwG_G
Cuts at: 0 87 275 383 810
Size: 87 188 108 427
Fragments arranged by size:
427 188 108 87
TatI w'GTAC_w
Cuts at: 0 146 412 810
Size: 146 266 398
Fragments arranged by size:
398 266 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 51 83 810
Size: 51 32 727
Fragments arranged by size:
727 51 32
Enzymes that do cut:
AcuI AleI AloI BanII BbsI BglI BplI BpmI
BpuEI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrGI
Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoRV
Hin4I MfeI MscI MslI NcoI NspI PpiI PstI
SfcI SfiI SmlI StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI