Restriction map for pFN21AE0801
 (Circular) (Six-base) MAPSORT of: pFN21AE0801.seq  Check: 3209  from: 1  to: 5773



pFN21AE0801  5773 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:51  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3583     278 
   Size:         53      83     186     936    2047    2468

  Fragments arranged by size: 

                2468    2047     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3116    1937 
   Size:       1179    4594
 
Acc65I G'GTAC_C

Cuts at:   3094    3094 
   Size:       5773
 
AclI AA'CG_TT

Cuts at:   1563    3900    4273    1563 
   Size:       2337     373    3063

  Fragments arranged by size: 

                3063    2337     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2025    3836    5220    5488    1632 
   Size:        393    1811    1384     268    1917

  Fragments arranged by size: 

                1917    1811    1384     393     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5576
 
AflIII A'CryG_T

Cuts at:   1203    4672    5649    1203 
   Size:       3469     977    1327

  Fragments arranged by size: 

                3469    1327     977
 
AgeI A'CCGG_T

Cuts at:   4604    4604 
   Size:       5773
 
AhdI GACnn_n'nnGTC

Cuts at:   4502    4502 
   Size:       5773
 
AleI CACnn'nnGTG

Cuts at:   1166    2972    1166 
   Size:       1806    3967
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5773
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2085    2117    2085 
   Size:         32    5741
 
AlwNI CAG_nnn'CTG

Cuts at:   3516    5088    3516 
   Size:       1572    4201
 
ApaLI G'TGCA_C

Cuts at:   2855    3830    4986    2855 
   Size:        975    1156    3642

  Fragments arranged by size: 

                3642    1156     975
 
ApoI r'AATT_y

Cuts at:   1493    2939    3082    3308    3460    5569    1493 
   Size:       1446     143     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1446     226     152     143
 
AseI AT'TA_AT

Cuts at:    160    1032    4327     160 
   Size:        872    3295    1606

  Fragments arranged by size: 

                3295    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5773
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3098    1870 
   Size:         73    1155    4545

  Fragments arranged by size: 

                4545    1155      73
 
BamHI G'GATC_C

Cuts at:   2499    3103    3470    2499 
   Size:        604     367    4802

  Fragments arranged by size: 

                4802     604     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3094    4549     618 
   Size:        325     217     627    1307    1455    1842

  Fragments arranged by size: 

                1842    1455    1307     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2069    3092    1391 
   Size:        678    1023    4072

  Fragments arranged by size: 

                4072    1023     678
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3045     928 
   Size:        971    1146    3656

  Fragments arranged by size: 

                3656    1146     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3964    3998    1903 
   Size:         34    2027      34    3678

  Fragments arranged by size: 

                3678    2027      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3665    4875    3665 
   Size:       1210    4563
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5773
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4384     136 
   Size:        107     122      71     631     605    2712    1525

  Fragments arranged by size: 

                2712    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5768    5768 
   Size:       5773
 
BlpI GC'TnA_GC

Cuts at:   3190    3190 
   Size:       5773
 
Bme1580I G_kGCm'C

Cuts at:   1790    2859    3834    4990    1790 
   Size:       1069     975    1156    2573

  Fragments arranged by size: 

                2573    1156    1069     975
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5773
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3504    4462     442 
   Size:        416      53      83     399      24    2087     958    1753

  Fragments arranged by size: 

                2087    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5773
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3052    4433    1239 
   Size:       1813    1381    2579

  Fragments arranged by size: 

                2579    1813    1381
 
Bpu10I CC'TnA_GC

Cuts at:   2068    2068 
   Size:       5773
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2714    2978    3898    4763    5061    2714 
   Size:        264     920     865     298    3426

  Fragments arranged by size: 

                3426     920     865     298     264
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4436     882 
   Size:        617    2937    2219

  Fragments arranged by size: 

                2937    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3564     493 
   Size:        711    2360    2702

  Fragments arranged by size: 

                2702    2360     711
 
BsaBI GATnn'nnATC

Cuts at:   2795    2870    5767    2795 
   Size:         75    2897    2801

  Fragments arranged by size: 

                2897    2801      75
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2493    3580    3962    4597 
   Size:         53      83     186     936     960    1087     382     635

Cuts at:   4597     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1087     960     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4206    4604    4878    5025    5436    4206 
   Size:        398     274     147     411    4543

  Fragments arranged by size: 

                4543     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2066    2096    1178 
   Size:         30     165      30     663      30    4855

  Fragments arranged by size: 

                4855     663     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2261    1186 
   Size:       1075    4698
 
BseYI C'CCAG_C

Cuts at:   2955    4976    5472    2955 
   Size:       2021     496    3256

  Fragments arranged by size: 

                3256    2021     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2234    1163 
   Size:        573     498    4702

  Fragments arranged by size: 

                4702     573     498
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3223    3984    5012    1540 
   Size:        275     186    1222     761    1028    2301

  Fragments arranged by size: 

                2301    1222    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2859    3834    3919    4990    2859 
   Size:        975      85    1071    3642

  Fragments arranged by size: 

                3642    1071     975      85
 
BsmI GAATG_Cn'

Cuts at:   3284    3377    3284 
   Size:         93    5680
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2069    2859    3092    3834    3919    4990    1391 
   Size:        399     279     790     233     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     790     742     399     279     233      85
            
 
BspEI T'CCGG_A

Cuts at:   5436    5436 
   Size:       5773
 
BspHI T'CATG_A

Cuts at:   3662    5392    3662 
   Size:       1730    4043
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3128     844 
   Size:       2284    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3660    1394 
   Size:        429    1837    3507

  Fragments arranged by size: 

                3507    1837     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2188    4268    4442      65 
   Size:       1183     940    2080     174    1396

  Fragments arranged by size: 

                2080    1396    1183     940     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4417    4604    1700 
   Size:        110    2607     187    2869

  Fragments arranged by size: 

                2869    2607     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5773
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5773
 
BssSI C'ACGA_G

Cuts at:   3833    4845    3833 
   Size:       1012    4761
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2306     794 
   Size:       1512    4261
 
BstBI TT'CG_AA

Cuts at:   4577    4577 
   Size:       5773
 
BstEII G'GTnAC_C

Cuts at:   1184    2207    1184 
   Size:       1023    4750
 
BstYI r'GATC_y

Cuts at:   1972    2499    2997    3103    3470    3855    3872    5324    5410 
   Size:        527     498     106     367     385      17    1452      86

Cuts at:   5410    5422    5768    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     527     498     385     367     346     106
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2310     513 
   Size:        552    1245    3976

  Fragments arranged by size: 

                3976    1245     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5034

  Fragments arranged by size: 

                5034     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2389    3289    4083    4103    2389 
   Size:        900     794      20    4059

  Fragments arranged by size: 

                4059     900     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5773
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5738
 
DraI TTT'AAA

Cuts at:   2944    3077    2944 
   Size:        133    5640
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4780     809 
   Size:        507    3464    1802

  Fragments arranged by size: 

                3464    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2262    2424    3220 
   Size:         54    1425      50     275      18     432     162     796

Cuts at:   3220    4109    5738       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     796     432     275     162      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3220    1537 
   Size:        275    1408    4090

  Fragments arranged by size: 

                4090    1408     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3703    1353 
   Size:       2350    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4360    4734    4880     387 
   Size:       1064     138    2771     374     146    1280

  Fragments arranged by size: 

                2771    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2025    3052    3836    4433    5220    5488    1239 
   Size:        393     393    1027     784     597     787     268    1524

  Fragments arranged by size: 

                1524    1027     787     784     597     393     393     268
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2342    2342 
   Size:       5773
 
EcoO109I rG'GnC_Cy

Cuts at:   2725    2881    2725 
   Size:        156    5617
 
EcoRI G'AATT_C

Cuts at:   3082    3460    3082 
   Size:        378    5395
 
FspI TGC'GCA

Cuts at:   4279    4279 
   Size:       5773
 
HaeII r_GCGC'y

Cuts at:   1678    4920    1678 
   Size:       3242    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2066    2098    2178    2210    3035 
   Size:         32     135      32     494      32      80      32     825

Cuts at:   3035    3067    4414    4446    4488    4520    1373 
   Size:         32    1347      32      42      32    2626

  Fragments arranged by size: 

                2626    1347     825     494     135      80      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2371    3117    3363     669 
   Size:        988     281     433     746     246    3079

  Fragments arranged by size: 

                3079     988     746     433     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5773
 
HpaI GTT'AAC

Cuts at:   3363    3363 
   Size:       5773
 
KpnI G_GTAC'C

Cuts at:   3098    3098 
   Size:       5773
 
MfeI C'AATT_G

Cuts at:   3372    3372 
   Size:       5773
 
MluI A'CGCG_T

Cuts at:   5649    5649 
   Size:       5773
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4887    5071    1305 
   Size:       3582     184    2007

  Fragments arranged by size: 

                3582    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2426      10 
   Size:         54    1425     343     594    3357

  Fragments arranged by size: 

                3357    1425     594     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2972    3731    4090    4249     518 
   Size:        648    1806     759     359     159    2042

  Fragments arranged by size: 

                2042    1806     759     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3189    3866    5014    5259    1606 
   Size:       1583     677    1148     245    2120

  Fragments arranged by size: 

                2120    1583    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5221
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5773
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5773
 
NotI GC'GGCC_GC

Cuts at:   3220    3220 
   Size:       5773
 
NspI r_CATG'y

Cuts at:   1132    1229    3131    4676    1132 
   Size:         97    1902    1545    2229

  Fragments arranged by size: 

                2229    1902    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5773
 
PciI A'CATG_T

Cuts at:   4672    4672 
   Size:       5773
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5773
 
PfoI T'CCnGG_A

Cuts at:   2814    2814 
   Size:       5773
 
PmeI GTTT'AAAC

Cuts at:   3077    3077 
   Size:       5773
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3842    3874    5355    5387    3842 
   Size:         32    1481      32    4228

  Fragments arranged by size: 

                4228    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2738    1613 
   Size:       1125    4648
 
PsiI TTA'TAA

Cuts at:   3343    3343 
   Size:       5773
 
PstI C_TGCA'G

Cuts at:    830    3125     830 
   Size:       2295    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5773
 
SalI G'TCGA_C

Cuts at:   1936    3115    1936 
   Size:       1179    4594
 
SbfI CC_TGCA'GG

Cuts at:   3125    3125 
   Size:       5773
 
ScaI AGT'ACT

Cuts at:   4021    5733    4021 
   Size:       1712    4061
 
SfcI C'TryA_G

Cuts at:    826    1045    2377    3121    4256    4937    5128     826 
   Size:        219    1332     744    1135     681     191    1471

  Fragments arranged by size: 

                1471    1332    1135     744     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5773
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5773
 
SmaI CCC'GGG

Cuts at:   1872    3100    1872 
   Size:       1228    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2729    2993    3877    4778    5040     820 
   Size:        197     926     786     264     884     901     262    1553

  Fragments arranged by size: 

                1553     926     901     884     786     264     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3564     493 
   Size:       3071    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5773
 
SphI G_CATG'C

Cuts at:   1132    3131    1132 
   Size:       1999    3774
 
SspI AAT'ATT

Cuts at:      5      52    3697       5 
   Size:         47    3645    2081

  Fragments arranged by size: 

                3645    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5773
 
StyI C'CwwG_G

Cuts at:    513    1065    2456    2703    2929    3212     513 
   Size:        552    1391     247     226     283    3074

  Fragments arranged by size: 

                3074    1391     552     283     247     226
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2590    3800    3817    3970    4155    4600     325 
   Size:       1255    1010    1210      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1210    1010     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2241    4019    5731 
   Size:        274      80      33      51    1443     264    1778    1712

Cuts at:   5731      96 
   Size:        138

  Fragments arranged by size: 

                1778    1712    1443     274     264     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5733    5765    5733 
   Size:         32    5741
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5773
 
XbaI T'CTAG_A

Cuts at:   3109    3109 
   Size:       5773
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5773
 
XmaI C'CCGG_G

Cuts at:   1870    3098    1870 
   Size:       1228    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3902    3902 
   Size:       5773
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3581     276 
   Size:         53      83     186     936    2047    2468

  Fragments arranged by size: 

                2468    2047     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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