(Circular) (Six-base) MAPSORT of: pFN21AE0801.seq Check: 3209 from: 1 to: 5773 pFN21AE0801 5773 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2025 3836 5220 5488 1632 Size: 393 1811 1384 268 1917 Fragments arranged by size: 1917 1811 1384 393 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 2972 1166 Size: 1806 3967 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2085 Size: 32 5741 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaLI G'TGCA_C Cuts at: 2855 3830 4986 2855 Size: 975 1156 3642 Fragments arranged by size: 3642 1156 975 ApoI r'AATT_y Cuts at: 1493 2939 3082 3308 3460 5569 1493 Size: 1446 143 226 152 2109 1697 Fragments arranged by size: 2109 1697 1446 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 3098 1870 Size: 73 1155 4545 Fragments arranged by size: 4545 1155 73 BamHI G'GATC_C Cuts at: 2499 3103 3470 2499 Size: 604 367 4802 Fragments arranged by size: 4802 604 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3094 4549 618 Size: 325 217 627 1307 1455 1842 Fragments arranged by size: 1842 1455 1307 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 3092 1391 Size: 678 1023 4072 Fragments arranged by size: 4072 1023 678 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3045 928 Size: 971 1146 3656 Fragments arranged by size: 3656 1146 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4384 136 Size: 107 122 71 631 605 2712 1525 Fragments arranged by size: 2712 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2859 3834 4990 1790 Size: 1069 975 1156 2573 Fragments arranged by size: 2573 1156 1069 975 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3052 4433 1239 Size: 1813 1381 2579 Fragments arranged by size: 2579 1813 1381 Bpu10I CC'TnA_GC Cuts at: 2068 2068 Size: 5773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 2978 3898 4763 5061 2714 Size: 264 920 865 298 3426 Fragments arranged by size: 3426 920 865 298 264 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 882 Size: 617 2937 2219 Fragments arranged by size: 2937 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 2795 2870 5767 2795 Size: 75 2897 2801 Fragments arranged by size: 2897 2801 75 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 3580 3962 4597 Size: 53 83 186 936 960 1087 382 635 Cuts at: 4597 275 Size: 1451 Fragments arranged by size: 1451 1087 960 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 1178 Size: 30 165 30 663 30 4855 Fragments arranged by size: 4855 663 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 1186 Size: 1075 4698 BseYI C'CCAG_C Cuts at: 2955 4976 5472 2955 Size: 2021 496 3256 Fragments arranged by size: 3256 2021 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 1163 Size: 573 498 4702 Fragments arranged by size: 4702 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2859 3834 3919 4990 2859 Size: 975 85 1071 3642 Fragments arranged by size: 3642 1071 975 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2859 3092 3834 3919 4990 1391 Size: 399 279 790 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 399 279 233 85 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3128 844 Size: 2284 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 4268 4442 65 Size: 1183 940 2080 174 1396 Fragments arranged by size: 2080 1396 1183 940 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4417 4604 1700 Size: 110 2607 187 2869 Fragments arranged by size: 2869 2607 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 2306 794 Size: 1512 4261 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 2207 1184 Size: 1023 4750 BstYI r'GATC_y Cuts at: 1972 2499 2997 3103 3470 3855 3872 5324 5410 Size: 527 498 106 367 385 17 1452 86 Cuts at: 5410 5422 5768 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 527 498 385 367 346 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2310 513 Size: 552 1245 3976 Fragments arranged by size: 3976 1245 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 2389 3289 4083 4103 2389 Size: 900 794 20 4059 Fragments arranged by size: 4059 900 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 2944 3077 2944 Size: 133 5640 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2424 3220 Size: 54 1425 50 275 18 432 162 796 Cuts at: 3220 4109 5738 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 796 432 275 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3703 1353 Size: 2350 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4360 4734 4880 387 Size: 1064 138 2771 374 146 1280 Fragments arranged by size: 2771 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2025 3052 3836 4433 5220 5488 1239 Size: 393 393 1027 784 597 787 268 1524 Fragments arranged by size: 1524 1027 787 784 597 393 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2342 Size: 5773 EcoO109I rG'GnC_Cy Cuts at: 2725 2881 2725 Size: 156 5617 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 4920 1678 Size: 3242 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2178 2210 3035 Size: 32 135 32 494 32 80 32 825 Cuts at: 3035 3067 4414 4446 4488 4520 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 825 494 135 80 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2371 3117 3363 669 Size: 988 281 433 746 246 3079 Fragments arranged by size: 3079 988 746 433 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4887 5071 1305 Size: 3582 184 2007 Fragments arranged by size: 3582 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 10 Size: 54 1425 343 594 3357 Fragments arranged by size: 3357 1425 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2972 3731 4090 4249 518 Size: 648 1806 759 359 159 2042 Fragments arranged by size: 2042 1806 759 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3189 3866 5014 5259 1606 Size: 1583 677 1148 245 2120 Fragments arranged by size: 2120 1583 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5221 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5773 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2738 1613 Size: 1125 4648 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PstI C_TGCA'G Cuts at: 830 3125 830 Size: 2295 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2377 3121 4256 4937 5128 826 Size: 219 1332 744 1135 681 191 1471 Fragments arranged by size: 1471 1332 1135 744 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 3100 1872 Size: 1228 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 2993 3877 4778 5040 820 Size: 197 926 786 264 884 901 262 1553 Fragments arranged by size: 1553 926 901 884 786 264 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 2456 2703 2929 3212 513 Size: 552 1391 247 226 283 3074 Fragments arranged by size: 3074 1391 552 283 247 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2590 3800 3817 3970 4155 4600 325 Size: 1255 1010 1210 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1210 1010 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 4019 5731 Size: 274 80 33 51 1443 264 1778 1712 Cuts at: 5731 96 Size: 138 Fragments arranged by size: 1778 1712 1443 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 3098 1870 Size: 1228 4545 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI