(Circular) (Six-base) MAPSORT of: pFN21AE0791.seq Check: 4213 from: 1 to: 5776 pFN21AE0791 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2308 2410 3839 5223 5491 1632 Size: 676 102 1429 1384 268 1917 Fragments arranged by size: 1917 1429 1384 676 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2827 4675 5652 1203 Size: 1624 1848 977 1327 Fragments arranged by size: 1848 1624 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 2703 1166 Size: 1537 4239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 3519 5091 3519 Size: 1572 4204 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2539 2732 3085 3311 3463 5572 1493 Size: 1046 193 353 226 152 2109 1697 Fragments arranged by size: 2109 1697 1046 353 226 193 152 AseI AT'TA_AT Cuts at: 160 1032 3056 4330 160 Size: 872 2024 1274 1606 Fragments arranged by size: 2024 1606 1274 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2413 3101 1870 Size: 73 470 688 4545 Fragments arranged by size: 4545 688 470 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2616 2649 2616 Size: 33 5743 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2841 3095 1391 Size: 1450 254 4072 Fragments arranged by size: 4072 1450 254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2270 928 Size: 971 161 210 4434 Fragments arranged by size: 4434 971 210 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 2089 2928 1548 Size: 541 839 4396 Fragments arranged by size: 4396 839 541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2319 5771 2319 Size: 3452 2324 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 3837 4993 1790 Size: 2047 1156 2573 Fragments arranged by size: 2573 2047 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2258 2507 1056 Size: 1202 249 4325 Fragments arranged by size: 4325 1202 249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4436 1239 Size: 3197 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 1178 Size: 30 165 30 1143 30 4378 Fragments arranged by size: 4378 1143 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 1186 Size: 1381 4395 BseYI C'CCAG_C Cuts at: 4979 5475 4979 Size: 496 5280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2841 3837 3922 4993 2841 Size: 996 85 1071 3624 Fragments arranged by size: 3624 1071 996 85 BsmI GAATG_Cn' Cuts at: 2793 3287 3380 2793 Size: 494 93 5189 Fragments arranged by size: 5189 494 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2841 3095 3837 3922 4993 1391 Size: 399 1051 254 742 85 1071 2174 Fragments arranged by size: 2174 1071 1051 742 399 254 85 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3048 3665 5395 3048 Size: 617 1730 3429 Fragments arranged by size: 3429 1730 617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2319 2708 3011 3106 3473 3858 3875 5327 Size: 347 389 303 95 367 385 17 1452 Cuts at: 5327 5413 5425 5771 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 389 385 367 347 346 303 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 3832 Fragments arranged by size: 3832 939 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3037 3080 3037 Size: 43 5733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 3223 4112 Size: 54 1425 50 275 18 270 1123 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1123 889 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 3706 1353 Size: 755 1598 3423 Fragments arranged by size: 3423 1598 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2160 4363 4737 4883 387 Size: 1064 138 571 2203 374 146 1280 Fragments arranged by size: 2203 1280 1064 571 374 146 138 EcoICRI GAG'CTC Cuts at: 2839 2839 Size: 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2308 2410 3839 4436 5223 5491 1239 Size: 393 676 102 1429 597 787 268 1524 Fragments arranged by size: 1524 1429 787 676 597 393 268 102 EcoO109I rG'GnC_Cy Cuts at: 2625 2625 Size: 5776 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 4417 4449 4491 Size: 32 135 32 1265 32 1548 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 1548 1265 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 3120 3366 669 Size: 988 281 139 1043 246 3079 Fragments arranged by size: 3079 1043 988 281 246 139 HindIII A'AGCT_T Cuts at: 748 2422 2873 748 Size: 1674 451 3651 Fragments arranged by size: 3651 1674 451 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2195 4890 5074 1305 Size: 890 2695 184 2007 Fragments arranged by size: 2695 2007 890 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2310 2703 3734 4093 4252 518 Size: 648 1144 393 1031 359 159 2042 Fragments arranged by size: 2042 1144 1031 648 393 359 159 MspA1I CmG'CkG Cuts at: 1606 3192 3869 5017 5262 1606 Size: 1586 677 1148 245 2120 Fragments arranged by size: 2120 1586 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 NdeI CA'TA_TG Cuts at: 387 2194 387 Size: 1807 3969 NheI G'CTAG_C Cuts at: 1052 2254 2503 1052 Size: 1202 249 4325 Fragments arranged by size: 4325 1202 249 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2276 2517 2831 2861 2943 3134 4679 Size: 97 1047 241 314 30 82 191 1545 Cuts at: 4679 1132 Size: 2229 Fragments arranged by size: 2229 1545 1047 314 241 191 97 82 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 2827 4675 2827 Size: 1848 3928 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2625 2625 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 SacI G_AGCT'C Cuts at: 2841 2841 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2108 2108 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2591 3124 4259 4940 5131 826 Size: 219 1546 533 1135 681 191 1471 Fragments arranged by size: 1546 1471 1135 681 533 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 2202 3700 5 Size: 47 2150 1498 2081 Fragments arranged by size: 2150 2081 1498 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 3215 513 Size: 552 939 177 1034 3074 Fragments arranged by size: 3074 1034 939 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2546 2578 5736 5768 2546 Size: 32 3158 32 2554 Fragments arranged by size: 3158 2554 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 2377 3112 2377 Size: 735 5041 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 2732 3905 2732 Size: 1173 4603 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI