Restriction map for
pFN21AE0788
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# NO CUT ENZYMES ( 112 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eco81I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MamI
MfeI MflI MluI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp509I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 416 TCCGGA T^CCGGA
AfaI 690 GTAC GT^AC
AlwI 80 GGATC GGATC(4/5)
ApaI 633 GGGCCC GGGCC^C
AspI 1200 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 140 TGGCCA TGG^CCA
BfaI 1217 CTAG C^TAG
BglI 844 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1101 GAGACC GGTCTC(1/5)
BsaWI 416 TCCGGA [AT]^CCGG[AT]
BseAI 416 TCCGGA T^CCGGA
BseRI 472 GAGGAG GAGGAG(10/8)
BsgI 538 GTGCAG GTGCAG(16/14)
BsiEI 795 CGGCCG CG[GA][CT]^CG
BsmBI 1150 GAGACG CGTCTC(1/5)
BsmI 650 GCATTC GAATGC(1/-1)
BspEI 416 TCCGGA T^CCGGA
BspHI 296 TCATGA T^CATGA
BsrDI 435 CATTGC GCAATG(2/0)
BsrI 1059 CCAGT ACTGG(1/-1)
DrdI 982 GACTTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 795 CGGCCG C^GGCCG
EclXI 795 CGGCCG C^GGCCG
Eco52I 795 CGGCCG C^GGCCG
EcoNI 1247 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 97 GATATC GAT^ATC
EcoT14I 584 CCATGG C^C[AT][AT]GG
HincII 234 GTCAAC GT[CT]^[GA]AC
HindII 234 GTCAAC GT[CT]^[GA]AC
KspI 1210 CCGCGG CCGC^GG
MaeI 1217 CTAG C^TAG
MaeIII 1206 GTCAC ^GT[ACGT]AC
MluNI 140 TGGCCA TGG^CCA
MroI 416 TCCGGA T^CCGGA
MscI 140 TGGCCA TGG^CCA
MseI 443 TTAA T^TAA
NaeI 832 GCCGGC GCC^GGC
NcoI 584 CCATGG C^CATGG
NgoMIV 832 GCCGGC G^CCGGC
NotI 794 GCGGCCGC GC^GGCCGC
NspI 218 ACATGC [GA]CATG^[CT]
PflFI 1200 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 216 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 989 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 296 TCATGA T^CATGA
RsaI 690 GTAC GT^AC
SacII 1210 CCGCGG CCGC^GG
SmlI 616 CTCAAG C^T[CT][GA]AG
StyI 584 CCATGG C^C[AT][AT]GG
TaqI 83 TCGA T^CGA
Tru9I 443 TTAA T^TAA
Tsp45I 1206 GTCAC ^GT[GC]AC
Tth111I 1200 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 83 TCGA T^CGA
Van91I 216 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 571 GACGTC GACGT^C
727 GACGTC
AhdI 1054 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1165 GACGTGGCGTC
AlwNI 22 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
280 CAGAAGCTG
Aor51HI 332 AGCGCT AGC^GCT
1142 AGCGCT
AspEI 1054 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1165 GACGTGGCGTC
AspHI 177 GAGCTC G[AT]GC[AT]^C
589 GAGCTC
AvaII 495 GGACC G^G[AT]CC
990 GGTCC
BpmI 358 CTGGAG CTGGAG(16/14)
1156 CTGGAG
BsiHKAI 177 GAGCTC G[AT]GC[AT]^C
589 GAGCTC
BsmAI 1101 GAGAC GTCTC(1/5)
1150 GAGAC
BsrBI 159 CCGCTC CCGCTC(-3/-3)
757 GAGCGG
BsrFI 424 ACCGGC [GA]^CCGG[CT]
832 GCCGGC
Cfr10I 424 ACCGGC [GA]^CCGG[CT]
832 GCCGGC
DsaI 584 CCATGG C^C[GA][CT]GG
1210 CCGCGG
Eam1105I 1054 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1165 GACGTGGCGTC
Eco47III 332 AGCGCT AGC^GCT
1142 AGCGCT
HinfI 808 GACTC G^A[ACGT]TC
1204 GAGTC
MslI 486 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
579 CATCGCCATG
PleI 808 GACTC GAGTC(4/5)
1204 GAGTC
PstI 127 CTGCAG CTGCA^G
286 CTGCAG
SacI 177 GAGCTC GAGCT^C
589 GAGCTC
SapI 513 GAAGAGC GCTCTTC(1/4)
1012 GAAGAGC
TspRI 541 CAGTG CA[GC]TG[ACGT][ACGT]^
668 CAGTG
XcmI 584 CCATGGAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
740 CCAACATCCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbsI BbvI BcnI
BmyI BpuAI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TseI