(Circular) (Six-base) MAPSORT of: pFN21AE0788.seq Check: 4245 from: 1 to: 5962 pFN21AE0788 5962 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2402 2615 3255 2402 Size: 213 640 5109 Fragments arranged by size: 5109 640 213 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2580 2736 3772 278 Size: 53 83 186 936 1044 156 1036 2468 Fragments arranged by size: 2468 1044 1036 936 186 156 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2537 2723 3241 4025 5409 5677 1632 Size: 443 462 186 518 784 1384 268 1917 Fragments arranged by size: 1917 1384 784 518 462 443 268 186 AfeI AGC'GCT Cuts at: 2339 3149 2339 Size: 810 5152 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 3064 3175 4691 3064 Size: 111 1516 4335 Fragments arranged by size: 4335 1516 111 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 2032 2290 3705 5277 2032 Size: 258 1415 1572 2717 Fragments arranged by size: 2717 1572 1415 258 ApaI G_GGCC'C Cuts at: 2642 2642 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2648 2861 3052 3287 1870 Size: 73 705 213 191 235 4545 Fragments arranged by size: 4545 705 235 213 191 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 2603 2857 3283 4738 Size: 325 217 627 603 213 254 426 1455 Cuts at: 4738 618 Size: 1842 Fragments arranged by size: 1842 1455 627 603 426 325 254 217 213 BanII G_rGCy'C Cuts at: 1391 2186 2235 2598 2642 3024 3281 1391 Size: 795 49 363 44 382 257 4072 Fragments arranged by size: 4072 795 382 363 257 49 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2432 2572 3209 928 Size: 971 533 140 637 3681 Fragments arranged by size: 3681 971 637 533 140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2855 4573 Size: 107 122 71 631 605 337 846 1718 Cuts at: 4573 136 Size: 1525 Fragments arranged by size: 1718 1525 846 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2606 2642 4023 5179 1790 Size: 816 36 1381 1156 2573 Fragments arranged by size: 2573 1381 1156 816 36 BmgBI CAC'GTC Cuts at: 1318 2437 3172 1318 Size: 1119 735 4108 Fragments arranged by size: 4108 1119 735 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 3182 4622 1239 Size: 1145 798 1440 2579 Fragments arranged by size: 2579 1440 1145 798 Bpu10I CC'TnA_GC Cuts at: 3233 3233 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 4087 4952 5250 2606 Size: 1481 865 298 3318 Fragments arranged by size: 3318 1481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3100 4625 882 Size: 617 1601 1525 2219 Fragments arranged by size: 2219 1601 1525 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2577 2733 3176 Size: 53 83 186 936 479 565 156 443 Cuts at: 3176 3228 3769 4151 4786 275 Size: 52 541 382 635 1451 Fragments arranged by size: 1451 936 635 565 541 479 443 382 186 156 83 53 52 BsaWI w'CCGG_w Cuts at: 2421 4395 4793 5067 5214 5625 2421 Size: 1974 398 274 147 411 2758 Fragments arranged by size: 2758 1974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 1186 Size: 1306 4656 BseYI C'CCAG_C Cuts at: 2112 2227 5165 5661 2112 Size: 115 2938 496 2413 Fragments arranged by size: 2938 2413 496 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 1163 Size: 573 828 4561 Fragments arranged by size: 4561 828 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3412 4173 5201 1540 Size: 275 186 802 609 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 609 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 2598 4023 4108 5179 2186 Size: 412 1425 85 1071 2969 Fragments arranged by size: 2969 1425 1071 412 85 BsmI GAATG_Cn' Cuts at: 2655 3473 3566 2655 Size: 818 93 5051 Fragments arranged by size: 5051 818 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3149 3149 Size: 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2235 2598 2606 2642 3024 3281 Size: 399 396 49 363 8 36 382 257 Cuts at: 3281 4023 4108 5179 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 382 363 257 85 49 36 8 BspEI T'CCGG_A Cuts at: 2421 5625 2421 Size: 3204 2758 BspHI T'CATG_A Cuts at: 2301 3851 5581 2301 Size: 1550 1730 2682 Fragments arranged by size: 2682 1730 1550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2402 2615 3255 3317 844 Size: 1558 213 640 62 3489 Fragments arranged by size: 3489 1558 640 213 62 BsrBI CCG'CTC Cuts at: 1394 1823 2166 2764 3849 1394 Size: 429 343 598 1085 3507 Fragments arranged by size: 3507 1085 598 429 343 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 4457 4631 65 Size: 1183 1191 2018 174 1396 Fragments arranged by size: 2018 1396 1191 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2429 2837 4606 4793 1700 Size: 110 619 408 1769 187 2869 Fragments arranged by size: 2869 1769 619 408 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957 Size: 1320 367 385 17 1452 86 12 346 Cuts at: 5957 1972 Size: 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2589 3215 513 Size: 552 939 585 626 3260 Fragments arranged by size: 3260 939 626 585 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2569 487 Size: 683 56 1343 3880 Fragments arranged by size: 3880 1343 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3478 4272 4292 2552 Size: 926 794 20 4222 Fragments arranged by size: 4222 926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2993 4969 809 Size: 507 1677 1976 1802 Fragments arranged by size: 1976 1802 1677 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2398 2431 Size: 54 1425 50 275 18 315 253 33 Cuts at: 2431 2800 3409 4298 5927 8 Size: 369 609 889 1629 43 Fragments arranged by size: 1629 1425 889 609 369 315 275 253 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2800 3409 1537 Size: 275 988 609 4090 Fragments arranged by size: 4090 988 609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 3012 3098 3892 1353 Size: 1160 499 86 794 3423 Fragments arranged by size: 3423 1160 794 499 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 4549 4923 5069 387 Size: 1064 138 506 2454 374 146 1280 Fragments arranged by size: 2454 1280 1064 506 374 146 138 EcoICRI GAG'CTC Cuts at: 2184 2596 2184 Size: 412 5550 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2384 2537 2723 3182 3241 4025 Size: 393 443 309 153 186 459 59 784 Cuts at: 4025 4622 5409 5677 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 784 597 459 443 393 309 268 186 153 59 EcoNI CCTnn'n_nnAGG Cuts at: 3256 3256 Size: 5962 EcoO109I rG'GnC_Cy Cuts at: 2638 2639 2995 2638 Size: 1 356 5605 Fragments arranged by size: 5605 356 1 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 EcoRV GAT'ATC Cuts at: 2104 2104 Size: 5962 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 3004 3101 2434 Size: 570 97 5295 Fragments arranged by size: 5295 570 97 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2341 2464 2693 2838 3151 5109 1678 Size: 663 123 229 145 313 1958 2531 Fragments arranged by size: 2531 1958 663 313 229 145 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3050 3082 3161 3193 4603 Size: 32 135 32 1478 32 79 32 1410 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1478 1410 135 79 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2241 3306 3552 669 Size: 988 281 303 1065 246 3079 Fragments arranged by size: 3079 1065 988 303 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2769 5076 5260 1305 Size: 1464 2307 184 2007 Fragments arranged by size: 2307 2007 1464 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 10 Size: 54 1425 343 315 3825 Fragments arranged by size: 3825 1425 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2588 3920 4279 4438 518 Size: 648 1329 93 1332 359 159 2042 Fragments arranged by size: 2042 1332 1329 648 359 159 93 MspA1I CmG'CkG Cuts at: 1606 2116 2143 2388 2799 3217 3378 4055 5203 Size: 510 27 245 411 418 161 677 1148 Cuts at: 5203 5448 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 510 418 411 245 245 161 27 NaeI GCC'GGC Cuts at: 2839 2839 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 2004 2589 513 Size: 552 939 585 3886 Fragments arranged by size: 3886 939 585 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 2837 2837 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 2800 3409 2800 Size: 609 5353 NspI r_CATG'y Cuts at: 1132 1229 2227 3320 4865 1132 Size: 97 998 1093 1545 2229 Fragments arranged by size: 2229 1545 1093 998 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2227 1108 Size: 1119 4843 PfoI T'CCnGG_A Cuts at: 2465 2465 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2995 2995 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2638 2638 Size: 5962 PstI C_TGCA'G Cuts at: 830 2136 2295 3314 830 Size: 1306 159 1019 3478 Fragments arranged by size: 3478 1306 1019 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SacI G_AGCT'C Cuts at: 2186 2598 2186 Size: 412 5550 SacII CC_GC'GG Cuts at: 3218 3218 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 3012 2513 Size: 499 5463 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2132 2291 3011 3310 4445 5126 5317 Size: 219 1087 159 720 299 1135 681 191 Cuts at: 5317 826 Size: 1471 Fragments arranged by size: 1471 1135 1087 720 681 299 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 4066 4967 5229 820 Size: 197 926 678 1445 901 262 1553 Fragments arranged by size: 1553 1445 926 901 678 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2589 3401 513 Size: 552 939 585 812 3074 Fragments arranged by size: 3074 939 812 585 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 4208 5920 Size: 274 80 33 51 1443 717 1514 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1514 1443 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 3208 1316 Size: 1892 4070 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2752 2596 Size: 156 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2578 2734 3770 276 Size: 53 83 186 936 1044 156 1036 2468 Fragments arranged by size: 2468 1044 1036 936 186 156 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI