Restriction map for
pFN21AE0695
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsgI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HphI KasI KspI
MaeII MamI MfeI MflI MluI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tth111I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 12 GGTACC G^GTACC
AccIII 70 TCCGGA T^CCGGA
AlwI 389 GGATC GGATC(4/5)
AlwNI 643 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 471 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 12 GGTACC G^GTACC
AspHI 66 GAGCTC G[AT]GC[AT]^C
BalI 780 TGGCCA TGG^CCA
BcnI 144 CCGGG CC^[GC]GG
BglI 226 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 63 GCTGAGC GC^T[ACGT]AGC
BpmI 266 CTCCAG CTGGAG(16/14)
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BseAI 70 TCCGGA T^CCGGA
BsiHKAI 66 GAGCTC G[AT]GC[AT]^C
BsmFI 754 GTCCC GGGAC(10/14)
BsmI 637 GCATTC GAATGC(1/-1)
BspEI 70 TCCGGA T^CCGGA
BstAPI 208 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 63 GCTGAGC GC^T[ACGT]AGC
DpnII 390 GATC ^GATC
DsaI 77 CCACGG C^C[GA][CT]GG
EagI 80 CGGCCG C^GGCCG
EarI 826 GAAGAG CTCTTC(1/4)
EclXI 80 CGGCCG C^GGCCG
Eco52I 80 CGGCCG C^GGCCG
EcoT22I 635 ATGCAT ATGCA^T
HinfI 519 GAGTC G^A[ACGT]TC
KpnI 12 GGTACC GGTAC^C
Ksp632I 826 GAAGAG CTCTTC(1/4)
MaeIII 38 GTGAC ^GT[ACGT]AC
MboI 390 GATC ^GATC
MluNI 780 TGGCCA TGG^CCA
MroI 70 TCCGGA T^CCGGA
MscI 780 TGGCCA TGG^CCA
NciI 144 CCGGG CC^[GC]GG
NdeII 390 GATC ^GATC
NsiI 635 ATGCAT ATGCA^T
NspI 235 GCATGC [GA]CATG^[CT]
PflMI 342 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 519 GAGTC GAGTC(4/5)
PstI 161 CTGCAG CTGCA^G
SacI 66 GAGCTC GAGCT^C
Sau3AI 390 GATC ^GATC
SfcI 161 CTGCAG C^T[GA][CT]AG
SphI 235 GCATGC GCATG^C
TaqI 32 TCGA T^CGA
Tsp45I 38 GTGAC ^GT[GC]AC
TthHB8I 32 TCGA T^CGA
Van91I 342 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 663 TCTAGA T^CTAGA
XmnI 471 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
22 GCGG
AfaI 13 GTAC GT^AC
690 GTAC
AvaII 73 GGACC G^G[AT]CC
568 GGACC
BanII 66 GAGCTC G[GA]GC[CT]^C
224 GAGCCC
BfaI 52 CTAG C^TAG
664 CTAG
BmyI 66 GAGCTC G[AGT]GC[ACT]^C
224 GAGCCC
BsaWI 70 TCCGGA [AT]^CCGG[AT]
242 ACCGGA
BsiEI 80 CGGCCG CG[GA][CT]^CG
240 CGACCG
Bsp1286I 66 GAGCTC G[AGT]GC[ACT]^C
224 GAGCCC
BstF5I 172 CATCC GGATG(2/0)
503 CATCC
Cac8I 235 GCATGC GC[ACGT]^[ACGT]GC
609 GCTTGC
DdeI 64 CTGAG C^T[ACGT]AG
119 CTCAG
EaeI 80 CGGCCG [CT]^GGCC[GA]
780 TGGCCA
FokI 172 CATCC GGATG(9/13)
503 CATCC
HindIII 397 AAGCTT A^AGCTT
607 AAGCTT
MaeI 52 CTAG C^TAG
664 CTAG
MseI 194 TTAA T^TAA
745 TTAA
NlaIII 236 CATG CATG^
444 CATG
RsaI 13 GTAC GT^AC
690 GTAC
Tru9I 194 TTAA T^TAA
745 TTAA
Tsp509I 355 AATT ^AATT
747 AATT
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
699 CAGTG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BanI BbsI BbvI BpuAI
BsaJI BseRI BsiYI BslI BsrI
BssKI BstNI Cfr13I EcoRII Fnu4HI
HaeIII HapII HpaII ItaI MboII
MnlI MspI MvaI MwoI NlaIV
Sau96I ScrFI SfaNI TseI