Restriction map for
pFN21AE0695
(Linear) (Six-base) MAPSORT of: KIEE0695.seq Check: 6615 from: 1 to: 852
KIEE0695.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
Acc65I G'GTAC_C
Cuts at: 0 12 852
Size: 12 840
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 490 852
Size: 385 105 362
Fragments arranged by size:
385 362 105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 814 846 852
Size: 814 32 6
Fragments arranged by size:
814 32 6
AlwNI CAG_nnn'CTG
Cuts at: 0 648 852
Size: 648 204
BanI G'GyrC_C
Cuts at: 0 12 286 447 852
Size: 12 274 161 405
Fragments arranged by size:
405 274 161 12
BanII G_rGCy'C
Cuts at: 0 70 228 852
Size: 70 158 624
Fragments arranged by size:
624 158 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 601 710 852
Size: 141 460 109 142
Fragments arranged by size:
460 142 141 109
BfrBI ATG'CAT
Cuts at: 0 637 852
Size: 637 215
BglI GCCn_nnn'nGGC
Cuts at: 0 232 852
Size: 232 620
BlpI GC'TnA_GC
Cuts at: 0 64 852
Size: 64 788
BmrI ACTGGGnnnn_n'
Cuts at: 0 692 852
Size: 692 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 84 852
Size: 84 768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 852
Size: 251 601
BsaWI w'CCGG_w
Cuts at: 0 70 242 852
Size: 70 172 610
Fragments arranged by size:
610 172 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 781 811 814 844 852
Size: 781 30 3 30 8
Fragments arranged by size:
781 30 30 8 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 556 802 835 852
Size: 556 246 33 17
Fragments arranged by size:
556 246 33 17
BsiEI CG_ry'CG
Cuts at: 0 83 243 852
Size: 83 160 609
Fragments arranged by size:
609 160 83
BsiHKAI G_wGCw'C
Cuts at: 0 70 852
Size: 70 782
BsmI GAATG_Cn'
Cuts at: 0 637 852
Size: 637 215
Bsp1286I G_dGCh'C
Cuts at: 0 70 228 852
Size: 70 158 624
Fragments arranged by size:
624 158 70
BspEI T'CCGG_A
Cuts at: 0 70 852
Size: 70 782
BstAPI GCAn_nnn'nTGC
Cuts at: 0 214 852
Size: 214 638
BtgI C'CryG_G
Cuts at: 0 77 852
Size: 77 775
BtsI GCAGTG_nn'
Cuts at: 0 170 852
Size: 170 682
EaeI y'GGCC_r
Cuts at: 0 80 780 852
Size: 80 700 72
Fragments arranged by size:
700 80 72
EagI C'GGCC_G
Cuts at: 0 80 852
Size: 80 772
EarI CTCTTCn'nnn_
Cuts at: 0 821 852
Size: 821 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 852
Size: 19 833
EcoICRI GAG'CTC
Cuts at: 0 68 852
Size: 68 784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 385 490 852
Size: 251 134 105 362
Fragments arranged by size:
362 251 134 105
HindIII A'AGCT_T
Cuts at: 0 397 607 852
Size: 397 210 245
Fragments arranged by size:
397 245 210
KpnI G_GTAC'C
Cuts at: 0 16 852
Size: 16 836
MscI TGG'CCA
Cuts at: 0 782 852
Size: 782 70
NsiI A_TGCA'T
Cuts at: 0 639 852
Size: 639 213
NspI r_CATG'y
Cuts at: 0 239 852
Size: 239 613
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 852
Size: 348 504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 852
Size: 814 32 6
Fragments arranged by size:
814 32 6
PstI C_TGCA'G
Cuts at: 0 165 852
Size: 165 687
SacI G_AGCT'C
Cuts at: 0 70 852
Size: 70 782
SfcI C'TryA_G
Cuts at: 0 161 852
Size: 161 691
SphI G_CATG'C
Cuts at: 0 239 852
Size: 239 613
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 365 852
Size: 365 487
TatI w'GTAC_w
Cuts at: 0 689 852
Size: 689 163
XbaI T'CTAG_A
Cuts at: 0 663 852
Size: 663 189
XmnI GAAnn'nnTTC
Cuts at: 0 475 852
Size: 475 377
Enzymes that do cut:
Acc65I AcuI AloI AlwNI BanI BanII BbsI BfrBI
BglI BlpI BmrI BplI BpmI BsaWI BsaXI BseRI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BstAPI BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI HindIII KpnI
MscI NsiI NspI PflMI PpiI PstI SacI SfcI
SphI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglII Bme1580I BmgBI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiWI BsmBI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HpaI
KasI MfeI MluI MmeI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI
SwaI TstI Tth111I XcmI XhoI XmaI ZraI