Restriction map for
pFN21AE0688
(Linear) (Six-base) MAPSORT of: KIEE0688.seq Check: 6736 from: 1 to: 1203
KIEE0688.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 190 256 1203
Size: 73 117 66 947
Fragments arranged by size:
947 117 73 66
AflII C'TTAA_G
Cuts at: 0 898 1203
Size: 898 305
AlwNI CAG_nnn'CTG
Cuts at: 0 463 759 780 1203
Size: 463 296 21 423
Fragments arranged by size:
463 423 296 21
ApoI r'AATT_y
Cuts at: 0 1028 1072 1171 1203
Size: 1028 44 99 32
Fragments arranged by size:
1028 99 44 32
AvaI C'yCGr_G
Cuts at: 0 566 1203
Size: 566 637
AvrII C'CTAG_G
Cuts at: 0 18 1203
Size: 18 1185
BamHI G'GATC_C
Cuts at: 0 240 1203
Size: 240 963
BanI G'GyrC_C
Cuts at: 0 1036 1203
Size: 1036 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 602 811 965 1203
Size: 602 209 154 238
Fragments arranged by size:
602 238 209 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 813 847 1203
Size: 813 34 356
Fragments arranged by size:
813 356 34
BfrBI ATG'CAT
Cuts at: 0 859 1203
Size: 859 344
BglII A'GATC_T
Cuts at: 0 661 1203
Size: 661 542
Bme1580I G_kGCm'C
Cuts at: 0 709 1041 1203
Size: 709 332 162
Fragments arranged by size:
709 332 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 245 628 1203
Size: 151 94 383 575
Fragments arranged by size:
575 383 151 94
Bpu10I CC'TnA_GC
Cuts at: 0 285 781 1203
Size: 285 496 422
Fragments arranged by size:
496 422 285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1203
Size: 576 627
BsaI GGTCTCn'nnnn_
Cuts at: 0 22 441 1203
Size: 22 419 762
Fragments arranged by size:
762 419 22
BsaBI GATnn'nnATC
Cuts at: 0 878 1203
Size: 878 325
BsaHI Gr'CG_yC
Cuts at: 0 842 1203
Size: 842 361
BsaWI w'CCGG_w
Cuts at: 0 670 1203
Size: 670 533
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 174 507 1203
Size: 174 333 696
Fragments arranged by size:
696 333 174
BseYI C'CCAG_C
Cuts at: 0 229 1203
Size: 229 974
BsiHKAI G_wGCw'C
Cuts at: 0 787 1203
Size: 787 416
Bsp1286I G_dGCh'C
Cuts at: 0 709 787 1041 1203
Size: 709 78 254 162
Fragments arranged by size:
709 254 162 78
BsrDI GCAATG_nn'
Cuts at: 0 521 1203
Size: 521 682
BsrFI r'CCGG_y
Cuts at: 0 194 1203
Size: 194 1009
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 1203
Size: 540 663
BstYI r'GATC_y
Cuts at: 0 240 453 661 1203
Size: 240 213 208 542
Fragments arranged by size:
542 240 213 208
BtsI GCAGTG_nn'
Cuts at: 0 785 1203
Size: 785 418
EarI CTCTTCn'nnn_
Cuts at: 0 578 1203
Size: 578 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 527 1203
Size: 527 676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 190 256 1203
Size: 73 117 66 947
Fragments arranged by size:
947 117 73 66
EcoO109I rG'GnC_Cy
Cuts at: 0 777 1203
Size: 777 426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 756 1203
Size: 756 447
HaeII r_GCGC'y
Cuts at: 0 342 1203
Size: 342 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 1203
Size: 438 32 733
Fragments arranged by size:
733 438 32
HincII GTy'rAC
Cuts at: 0 1110 1203
Size: 1110 93
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 987 1203
Size: 691 296 216
Fragments arranged by size:
691 296 216
MspA1I CmG'CkG
Cuts at: 0 229 366 885 1203
Size: 229 137 519 318
Fragments arranged by size:
519 318 229 137
NsiI A_TGCA'T
Cuts at: 0 861 1203
Size: 861 342
NspI r_CATG'y
Cuts at: 0 223 1154 1203
Size: 223 931 49
Fragments arranged by size:
931 223 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 759 1203
Size: 759 444
PfoI T'CCnGG_A
Cuts at: 0 743 1203
Size: 743 460
PstI C_TGCA'G
Cuts at: 0 80 267 1203
Size: 80 187 936
Fragments arranged by size:
936 187 80
PvuII CAG'CTG
Cuts at: 0 229 1203
Size: 229 974
SfcI C'TryA_G
Cuts at: 0 76 263 1203
Size: 76 187 940
Fragments arranged by size:
940 187 76
SmlI C'TyrA_G
Cuts at: 0 591 898 1203
Size: 591 307 305
Fragments arranged by size:
591 307 305
StyI C'CwwG_G
Cuts at: 0 18 977 1203
Size: 18 959 226
Fragments arranged by size:
959 226 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 546 672 1203
Size: 546 126 531
Fragments arranged by size:
546 531 126
TatI w'GTAC_w
Cuts at: 0 142 1203
Size: 142 1061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1001 1033 1203
Size: 1001 32 170
Fragments arranged by size:
1001 170 32
Enzymes that do cut:
AcuI AflII AlwNI ApoI AvaI AvrII BamHI BanI
BbsI BcgI BfrBI BglII Bme1580I BmrI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I
BsrDI BsrFI BstXI BstYI BtsI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII MmeI MspA1I NsiI
NspI PflMI PfoI PstI PvuII SfcI SmlI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI BaeI BanII BbeI BbvCI BciVI BclI BglI
BlpI BmgBI BmtI BplI BpmI BsaAI BsaXI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI