(Circular) (Six-base) MAPSORT of: pFN21AE0682.seq Check: 2673 from: 1 to: 5872 pFN21AE0682 5872 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2781 3215 1937 Size: 844 434 4594 Fragments arranged by size: 4594 844 434 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2050 3113 3935 5319 5587 1632 Size: 418 1063 822 1384 268 1917 Fragments arranged by size: 1917 1384 1063 822 418 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AlwNI CAG_nnn'CTG Cuts at: 2103 2113 2282 2500 3615 5187 2103 Size: 10 169 218 1115 1572 2788 Fragments arranged by size: 2788 1572 1115 218 169 10 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 3181 3407 3559 5668 1493 Size: 1688 226 152 2109 1697 Fragments arranged by size: 2109 1697 1688 226 152 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2226 3197 1870 Size: 73 283 971 4545 Fragments arranged by size: 4545 971 283 73 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2188 2451 2958 3193 4648 Size: 325 217 627 401 263 507 235 1455 Cuts at: 4648 618 Size: 1842 Fragments arranged by size: 1842 1455 627 507 401 325 263 235 217 BanII G_rGCy'C Cuts at: 1391 2337 2559 2721 3191 1391 Size: 946 222 162 470 4072 Fragments arranged by size: 4072 946 470 222 162 BbeI G_GCGC'C Cuts at: 2455 2455 Size: 5872 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2249 928 Size: 971 350 4551 Fragments arranged by size: 4551 971 350 BbvCI CC'TCA_GC Cuts at: 2763 2763 Size: 5872 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 4483 136 Size: 107 122 71 631 605 475 2336 1525 Fragments arranged by size: 2336 1525 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 2528 3289 2528 Size: 761 5111 Bme1580I G_kGCm'C Cuts at: 1790 2961 3933 5089 1790 Size: 1171 972 1156 2573 Fragments arranged by size: 2573 1171 1156 972 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2641 2675 3603 Size: 416 53 83 399 24 1224 34 928 Cuts at: 3603 4561 442 Size: 958 1753 Fragments arranged by size: 1753 1224 958 928 416 399 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 2351 3109 4532 1239 Size: 975 137 758 1423 2579 Fragments arranged by size: 2579 1423 975 758 137 Bpu10I CC'TnA_GC Cuts at: 2441 2577 2732 2763 2441 Size: 136 155 31 5550 Fragments arranged by size: 5550 155 136 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3997 4862 5160 3997 Size: 865 298 4709 Fragments arranged by size: 4709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 4535 882 Size: 617 717 2319 2219 Fragments arranged by size: 2319 2219 717 617 BsaAI yAC'GTr Cuts at: 493 1204 2458 3663 493 Size: 711 1254 1205 2702 Fragments arranged by size: 2702 1254 1205 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2452 3679 4061 4696 Size: 53 83 186 936 919 1227 382 635 Cuts at: 4696 275 Size: 1451 Fragments arranged by size: 1451 1227 936 919 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3084 3114 1178 Size: 30 165 30 1681 30 3936 Fragments arranged by size: 3936 1681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 3156 1186 Size: 1012 958 3902 Fragments arranged by size: 3902 1012 958 BseYI C'CCAG_C Cuts at: 2436 2464 2724 2906 3021 5075 5571 2436 Size: 28 260 182 115 2054 496 2737 Fragments arranged by size: 2737 2054 496 260 182 115 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 1163 Size: 573 756 4543 Fragments arranged by size: 4543 756 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3933 4018 5089 3933 Size: 85 1071 4716 Fragments arranged by size: 4716 1071 85 BsmI GAATG_Cn' Cuts at: 2173 3028 3383 3476 2173 Size: 855 355 93 4569 Fragments arranged by size: 4569 855 355 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2537 2537 Size: 5872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2559 2721 2961 3191 3933 4018 Size: 399 547 222 162 240 230 742 85 Cuts at: 4018 5089 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 547 399 240 230 222 162 85 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 3761 5491 3761 Size: 1730 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 3227 844 Size: 1664 719 3489 Fragments arranged by size: 3489 1664 719 BsrBI CCG'CTC Cuts at: 1394 1823 2527 3759 1394 Size: 429 704 1232 3507 Fragments arranged by size: 3507 1232 704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4367 4541 65 Size: 1183 3119 174 1396 Fragments arranged by size: 3119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 2103 2282 2500 794 Size: 1309 179 218 4166 Fragments arranged by size: 4166 1309 218 179 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 2380 1184 Size: 1196 4676 BstXI CCAn_nnnn'nTGG Cuts at: 3087 3087 Size: 5872 BstYI r'GATC_y Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867 Size: 1230 367 385 17 1452 86 12 346 Cuts at: 5867 1972 Size: 1977 Fragments arranged by size: 1977 1452 1230 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2343 2597 3168 2343 Size: 254 571 5047 Fragments arranged by size: 5047 571 254 BtgI C'CryG_G Cuts at: 513 1065 2004 2293 2877 513 Size: 552 939 289 584 3508 Fragments arranged by size: 3508 939 584 552 289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5133 Fragments arranged by size: 5133 683 56 BtsI GCAGTG_nn' Cuts at: 2121 3388 4182 4202 2121 Size: 1267 794 20 3791 Fragments arranged by size: 3791 1267 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DraIII CAC_nnn'GTG Cuts at: 2461 2461 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3319 4208 5837 Size: 54 1425 50 275 18 1489 889 1629 Cuts at: 5837 8 Size: 43 Fragments arranged by size: 1629 1489 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2969 3802 1353 Size: 1616 833 3423 Fragments arranged by size: 3423 1616 833 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2214 2351 3109 3113 3935 4532 Size: 393 418 164 137 758 4 822 597 Cuts at: 4532 5319 5587 1239 Size: 787 268 1524 Fragments arranged by size: 1524 822 787 758 597 418 393 268 164 137 4 EcoNI CCTnn'n_nnAGG Cuts at: 2196 2994 2196 Size: 798 5074 EcoO109I rG'GnC_Cy Cuts at: 2140 2497 2635 2938 3056 2140 Size: 357 138 303 118 4956 Fragments arranged by size: 4956 357 303 138 118 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2455 2481 5019 1678 Size: 777 26 2538 2531 Fragments arranged by size: 2538 2531 777 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3084 3116 4513 4545 4587 Size: 32 135 32 1512 32 1397 32 42 Cuts at: 4587 4619 1373 Size: 32 2626 Fragments arranged by size: 2626 1512 1397 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KasI G'GCGC_C Cuts at: 2451 2451 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 4986 5170 1305 Size: 876 2805 184 2007 Fragments arranged by size: 2805 2007 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2918 3830 4189 4348 518 Size: 648 921 831 912 359 159 2042 Fragments arranged by size: 2042 921 912 831 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2728 2767 3025 3288 3965 5113 5358 1606 Size: 1122 39 258 263 677 1148 245 2120 Fragments arranged by size: 2120 1148 1122 677 263 258 245 39 NarI GG'CG_CC Cuts at: 2452 2452 Size: 5872 NcoI C'CATG_G Cuts at: 513 1065 2004 2293 2877 513 Size: 552 939 289 584 3508 Fragments arranged by size: 3508 939 584 552 289 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 3230 4775 1132 Size: 97 2001 1545 2229 Fragments arranged by size: 2229 2001 1545 97 PasI CC'CwG_GG Cuts at: 1348 2894 1348 Size: 1546 4326 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 1108 Size: 1005 4867 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PmlI CAC'GTG Cuts at: 2458 2458 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2635 2497 Size: 138 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2102 2134 2102 Size: 32 5840 PstI C_TGCA'G Cuts at: 830 2217 2268 2496 2587 3224 830 Size: 1387 51 228 91 637 3478 Fragments arranged by size: 3478 1387 637 228 91 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2728 2767 3025 2728 Size: 39 258 5575 Fragments arranged by size: 5575 258 39 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SapI GCTCTTCn'nnn_ Cuts at: 2969 2969 Size: 5872 SbfI CC_TGCA'GG Cuts at: 2217 2587 3224 2217 Size: 370 637 4865 Fragments arranged by size: 4865 637 370 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SfcI C'TryA_G Cuts at: 826 1045 2213 2264 2492 2583 3220 4355 5036 Size: 219 1168 51 228 91 637 1135 681 Cuts at: 5036 5227 826 Size: 191 1471 Fragments arranged by size: 1471 1168 1135 681 637 228 219 191 91 51 SfoI GGC'GCC Cuts at: 2453 2453 Size: 5872 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 2228 3199 1872 Size: 356 971 4545 Fragments arranged by size: 4545 971 356 SmlI C'TyrA_G Cuts at: 820 1017 1943 3976 4877 5139 820 Size: 197 926 2033 901 262 1553 Fragments arranged by size: 2033 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 2989 1517 Size: 1472 4400 StyI C'CwwG_G Cuts at: 513 1065 2004 2035 2293 2877 2953 3157 3311 Size: 552 939 31 258 584 76 204 154 Cuts at: 3311 513 Size: 3074 Fragments arranged by size: 3074 939 584 552 258 204 154 76 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2618 3899 3916 4069 4254 4699 325 Size: 1255 1038 1281 17 153 185 445 1498 Fragments arranged by size: 1498 1281 1255 1038 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 4118 5830 Size: 274 80 33 51 1443 672 1469 1712 Cuts at: 5830 96 Size: 138 Fragments arranged by size: 1712 1469 1443 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3116 3208 3116 Size: 92 5780 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3011 3011 Size: 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 2226 3197 1870 Size: 356 971 4545 Fragments arranged by size: 4545 971 356 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI