(Circular) (Six-base) MAPSORT of: pFN21AE0656.seq Check: 1388 from: 1 to: 5869 pFN21AE0656 5869 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2949 2949 Size: 5869 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3212 1937 Size: 1275 4594 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 2708 2804 2841 3932 5316 5584 1632 Size: 782 294 96 37 1091 1384 268 1917 Fragments arranged by size: 1917 1384 1091 782 294 268 96 37 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 2037 2039 2859 4768 5745 1203 Size: 834 2 820 1909 977 1327 Fragments arranged by size: 1909 1327 977 834 820 2 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 2370 1166 Size: 1204 4665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5869 AlwNI CAG_nnn'CTG Cuts at: 3612 5184 3612 Size: 1572 4297 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 2030 2125 2807 2981 3178 3404 3556 5665 Size: 537 95 682 174 197 226 152 2109 Cuts at: 5665 1493 Size: 1697 Fragments arranged by size: 2109 1697 682 537 226 197 174 152 95 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 3194 1870 Size: 73 1251 4545 Fragments arranged by size: 4545 1251 73 AvrII C'CTAG_G Cuts at: 2338 2338 Size: 5869 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3190 4645 618 Size: 325 217 627 1403 1455 1842 Fragments arranged by size: 1842 1455 1403 627 325 217 BanII G_rGCy'C Cuts at: 1391 3188 1391 Size: 1797 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 1903 Size: 34 2123 34 3678 Fragments arranged by size: 3678 2123 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4971 3761 Size: 1210 4659 BclI T'GATC_A Cuts at: 1548 2269 1548 Size: 721 5148 BfrBI ATG'CAT Cuts at: 2659 2659 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4480 136 Size: 107 122 71 631 605 2808 1525 Fragments arranged by size: 2808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2147 5864 2147 Size: 3717 2152 BlpI GC'TnA_GC Cuts at: 2724 3286 2724 Size: 562 5307 Bme1580I G_kGCm'C Cuts at: 1790 3930 5086 1790 Size: 2140 1156 2573 Fragments arranged by size: 2573 2140 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3600 4558 442 Size: 416 53 83 399 24 2183 958 1753 Fragments arranged by size: 2183 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3034 3034 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 3091 4529 1239 Size: 842 1010 1438 2579 Fragments arranged by size: 2579 1438 1010 842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3056 3994 4859 5157 3056 Size: 938 865 298 3768 Fragments arranged by size: 3768 938 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4532 882 Size: 617 3033 2219 Fragments arranged by size: 3033 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2040 3660 493 Size: 711 836 1620 2702 Fragments arranged by size: 2702 1620 836 711 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3676 4058 4693 275 Size: 53 83 186 936 2143 382 635 1451 Fragments arranged by size: 2143 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4302 4700 4974 5121 5532 4302 Size: 398 274 147 411 4639 Fragments arranged by size: 4639 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 1178 Size: 30 165 30 759 30 4855 Fragments arranged by size: 4855 759 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2244 2358 2390 2456 3014 1186 Size: 1058 114 32 66 558 4041 Fragments arranged by size: 4041 1058 558 114 66 32 BseYI C'CCAG_C Cuts at: 5072 5568 5072 Size: 496 5373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2104 1163 Size: 573 368 4928 Fragments arranged by size: 4928 573 368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3319 4080 5108 1540 Size: 275 186 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3930 4015 5086 3930 Size: 85 1071 4713 Fragments arranged by size: 4713 1071 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 BsmBI CGTCTCn'nnnn_ Cuts at: 2372 2927 2372 Size: 555 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3188 3930 4015 5086 1391 Size: 399 1398 742 85 1071 2174 Fragments arranged by size: 2174 1398 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 2466 2625 3758 5488 2466 Size: 159 1133 1730 2847 Fragments arranged by size: 2847 1730 1133 159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2949 3224 844 Size: 2105 275 3489 Fragments arranged by size: 3489 2105 275 BsrBI CCG'CTC Cuts at: 1394 1823 2300 3756 1394 Size: 429 477 1456 3507 Fragments arranged by size: 3507 1456 477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4364 4538 65 Size: 1183 3116 174 1396 Fragments arranged by size: 3116 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4513 4700 1700 Size: 110 2703 187 2869 Fragments arranged by size: 2869 2703 187 110 BsrGI T'GTAC_A Cuts at: 96 2667 96 Size: 2571 3298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 3929 4941 3929 Size: 1012 4857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5869 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstYI r'GATC_y Cuts at: 1972 2147 3199 3566 3951 3968 5420 5506 5518 Size: 175 1052 367 385 17 1452 86 12 Cuts at: 5518 5864 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1052 385 367 346 175 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5317 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2863 487 Size: 683 56 1637 3493 Fragments arranged by size: 3493 1637 683 56 BtsI GCAGTG_nn' Cuts at: 3385 4179 4199 3385 Size: 794 20 5055 Fragments arranged by size: 5055 794 20 ClaI AT'CG_AT Cuts at: 1554 2583 1554 Size: 1029 4840 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2356 2382 2754 Size: 54 1425 50 275 18 526 26 372 Cuts at: 2754 3316 4205 5834 8 Size: 562 889 1629 43 Fragments arranged by size: 1629 1425 889 562 526 372 275 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 3316 1537 Size: 275 1504 4090 Fragments arranged by size: 4090 1504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2085 3035 3799 1353 Size: 659 73 950 764 3423 Fragments arranged by size: 3423 950 764 659 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4456 4830 4976 387 Size: 1064 138 2867 374 146 1280 Fragments arranged by size: 2867 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2414 2708 2804 2841 3091 3932 Size: 393 449 333 294 96 37 250 841 Cuts at: 3932 4529 5316 5584 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 841 787 597 449 393 333 294 268 250 96 37 EcoRI G'AATT_C Cuts at: 2030 3178 3556 2030 Size: 1148 378 4343 Fragments arranged by size: 4343 1148 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 5869 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 5016 1678 Size: 3338 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2162 2164 2194 4510 Size: 32 135 32 560 30 2 30 2316 Cuts at: 4510 4542 4584 4616 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2316 560 135 42 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2242 2305 3213 3459 669 Size: 988 281 304 63 908 246 3079 Fragments arranged by size: 3079 988 908 304 281 246 63 HindIII A'AGCT_T Cuts at: 748 3023 748 Size: 2275 3594 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 10 Size: 54 1425 343 924 3123 Fragments arranged by size: 3123 1425 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2370 2858 3827 4186 4345 518 Size: 648 1204 488 969 359 159 2042 Fragments arranged by size: 2042 1204 969 648 488 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2476 2500 3285 3962 5110 5355 1606 Size: 453 417 24 785 677 1148 245 2120 Fragments arranged by size: 2120 1148 785 677 453 417 245 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5317 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NsiI A_TGCA'T Cuts at: 2661 2661 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 2659 2863 3227 4772 1132 Size: 97 1430 204 364 1545 2229 Fragments arranged by size: 2229 1545 1430 364 204 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 2859 4768 2859 Size: 1909 3960 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5869 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PmlI CAC'GTG Cuts at: 2040 2040 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 2046 3439 2046 Size: 1393 4476 PstI C_TGCA'G Cuts at: 830 2960 3221 830 Size: 2130 261 3478 Fragments arranged by size: 3478 2130 261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 PvuII CAG'CTG Cuts at: 2059 2476 2500 2059 Size: 417 24 5428 Fragments arranged by size: 5428 417 24 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SbfI CC_TGCA'GG Cuts at: 2960 3221 2960 Size: 261 5608 ScaI AGT'ACT Cuts at: 4117 5829 4117 Size: 1712 4157 SfcI C'TryA_G Cuts at: 826 1045 2956 3217 4352 5033 5224 826 Size: 219 1911 261 1135 681 191 1471 Fragments arranged by size: 1911 1471 1135 681 261 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3071 3973 4874 5136 820 Size: 197 926 1128 902 901 262 1553 Fragments arranged by size: 1553 1128 926 902 901 262 197 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 2338 3006 3308 513 Size: 552 1273 668 302 3074 Fragments arranged by size: 3074 1273 668 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2906 3896 3913 4066 4251 4696 325 Size: 1255 1326 990 17 153 185 445 1498 Fragments arranged by size: 1498 1326 1255 990 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 4115 5827 Size: 274 80 33 51 1443 690 1448 1712 Cuts at: 5827 96 Size: 138 Fragments arranged by size: 1712 1448 1443 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5829 5861 5829 Size: 32 5837 Tth111I GACn'n_nGTC Cuts at: 1316 2531 1316 Size: 1215 4654 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 2982 3998 2982 Size: 1016 4853 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI