Restriction map for
pFN21AE0656
(Linear) (Six-base) MAPSORT of: KIEE0656.seq Check: 4247 from: 1 to: 1164
KIEE0656.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 944 1164
Size: 944 220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 703 799 836 1164
Size: 409 294 96 37 328
Fragments arranged by size:
409 328 294 96 37
AflIII A'CryG_T
Cuts at: 0 32 34 854 1164
Size: 32 2 820 310
Fragments arranged by size:
820 310 32 2
AleI CACnn'nnGTG
Cuts at: 0 365 1164
Size: 365 799
ApoI r'AATT_y
Cuts at: 0 25 120 802 976 1164
Size: 25 95 682 174 188
Fragments arranged by size:
682 188 174 95 25
AvrII C'CTAG_G
Cuts at: 0 333 1164
Size: 333 831
BclI T'GATC_A
Cuts at: 0 264 1164
Size: 264 900
BfrBI ATG'CAT
Cuts at: 0 654 1164
Size: 654 510
BglII A'GATC_T
Cuts at: 0 142 1164
Size: 142 1022
BlpI GC'TnA_GC
Cuts at: 0 719 1164
Size: 719 445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 1164
Size: 1029 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1086 1164
Size: 76 1010 78
Fragments arranged by size:
1010 78 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1164
Size: 1051 113
BsaAI yAC'GTr
Cuts at: 0 35 1164
Size: 35 1129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 1164
Size: 157 30 977
Fragments arranged by size:
977 157 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 239 353 385 451 1009 1164
Size: 239 114 32 66 558 155
Fragments arranged by size:
558 239 155 114 66 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1164
Size: 99 1065
BsmBI CGTCTCn'nnnn_
Cuts at: 0 367 922 1164
Size: 367 555 242
Fragments arranged by size:
555 367 242
BspHI T'CATG_A
Cuts at: 0 461 620 1164
Size: 461 159 544
Fragments arranged by size:
544 461 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 944 1164
Size: 944 220
BsrBI CCG'CTC
Cuts at: 0 295 1164
Size: 295 869
BsrGI T'GTAC_A
Cuts at: 0 662 1164
Size: 662 502
BstYI r'GATC_y
Cuts at: 0 142 1164
Size: 142 1022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 858 1164
Size: 858 306
ClaI AT'CG_AT
Cuts at: 0 578 1164
Size: 578 586
EaeI y'GGCC_r
Cuts at: 0 351 377 749 1164
Size: 351 26 372 415
Fragments arranged by size:
415 372 351 26
EarI CTCTTCn'nnn_
Cuts at: 0 7 80 1030 1164
Size: 7 73 950 134
Fragments arranged by size:
950 134 73 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 409 703 799 836 1086 1164
Size: 76 333 294 96 37 250 78
Fragments arranged by size:
333 294 250 96 78 76 37
EcoRI G'AATT_C
Cuts at: 0 25 1164
Size: 25 1139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 1164
Size: 804 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 157 159 189 1164
Size: 127 30 2 30 975
Fragments arranged by size:
975 127 30 30 2
HincII GTy'rAC
Cuts at: 0 237 300 1164
Size: 237 63 864
Fragments arranged by size:
864 237 63
HindIII A'AGCT_T
Cuts at: 0 1018 1164
Size: 1018 146
MscI TGG'CCA
Cuts at: 0 751 1164
Size: 751 413
MslI CAynn'nnrTG
Cuts at: 0 365 853 1164
Size: 365 488 311
Fragments arranged by size:
488 365 311
MspA1I CmG'CkG
Cuts at: 0 54 471 495 1164
Size: 54 417 24 669
Fragments arranged by size:
669 417 54 24
NsiI A_TGCA'T
Cuts at: 0 656 1164
Size: 656 508
NspI r_CATG'y
Cuts at: 0 654 858 1164
Size: 654 204 306
Fragments arranged by size:
654 306 204
PciI A'CATG_T
Cuts at: 0 854 1164
Size: 854 310
PmlI CAC'GTG
Cuts at: 0 35 1164
Size: 35 1129
PsiI TTA'TAA
Cuts at: 0 41 1164
Size: 41 1123
PstI C_TGCA'G
Cuts at: 0 955 1164
Size: 955 209
PvuII CAG'CTG
Cuts at: 0 54 471 495 1164
Size: 54 417 24 669
Fragments arranged by size:
669 417 54 24
SbfI CC_TGCA'GG
Cuts at: 0 955 1164
Size: 955 209
SfcI C'TryA_G
Cuts at: 0 951 1164
Size: 951 213
SmlI C'TyrA_G
Cuts at: 0 1066 1164
Size: 1066 98
StyI C'CwwG_G
Cuts at: 0 333 1001 1164
Size: 333 668 163
Fragments arranged by size:
668 333 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 901 1164
Size: 901 263
TatI w'GTAC_w
Cuts at: 0 662 1164
Size: 662 502
Tth111I GACn'n_nGTC
Cuts at: 0 526 1164
Size: 526 638
XmnI GAAnn'nnTTC
Cuts at: 0 977 1164
Size: 977 187
Enzymes that do cut:
AarI AcuI AflIII AleI ApoI AvrII BclI BfrBI
BglII BlpI BplI BpmI BpuEI BsaAI BsaXI BseRI
BsgI BsmBI BspHI BspMI BsrBI BsrGI BstYI BtgZI
ClaI EaeI EarI Eco57MI EcoRI FalI Hin4I HincII
HindIII MscI MslI MspA1I NsiI NspI PciI PmlI
PsiI PstI PvuII SbfI SfcI SmlI StyI TaqII
TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI Bme1580I BmgBI BmrI BmtI Bpu10I
BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XcmI XhoI XmaI
ZraI