Restriction map for
pFN21AE0617
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tru9I
Tsp45I TspRI Tth111I Van91I XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 440 GTAGAC GT^[AC][GT]AC
AcsI 85 GAATTT [GA]^AATT[CT]
AflIII 26 ACACGT A^C[GA][CT]GT
AhdI 58 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 502 AGCT AG^CT
AlwI 264 GGATC GGATC(4/5)
ApoI 85 GAATTT [GA]^AATT[CT]
Asp700I 470 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 58 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 100 GGTCC G^G[AT]CC
BbvI 500 GCAGC GCAGC(8/12)
BseRI 378 GAGGAG GAGGAG(10/8)
BsiYI 293 CCAAGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 293 CCAAGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 30 GTCTC GTCTC(1/5)
BsmBI 29 CGTCTC CGTCTC(1/5)
Bsp1407I 311 TGTACA T^GTACA
BspMI 11 ACCTGC ACCTGC(4/8)
BsrGI 311 TGTACA T^GTACA
BstF5I 177 GGATG GGATG(2/0)
Cfr13I 100 GGTCC G^G[ACGT]CC
DsaI 457 CCACGG C^C[GA][CT]GG
EaeI 50 TGGCCG [CT]^GGCC[GA]
Eam1105I 58 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 293 CCAAGG C^C[AT][AT]GG
FauI 5 CCCGC CCCGC(4/6)
FokI 177 GGATG GGATG(9/13)
HaeIII 51 GGCC GG^CC
MspA1I 501 CAGCTG C[AC]G^C[GT]G
MwoI 190 GCTGAAGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI 498 CTGCAG CTGCA^G
PvuII 501 CAGCTG CAG^CTG
Sau96I 100 GGTCC G^G[ACGT]CC
SexAI 61 ACCAGGT A^CC[AT]GGT
SfaNI 187 GATGC GCATC(5/9)
SspBI 311 TGTACA T^GTACA
StyI 293 CCAAGG C^C[AT][AT]GG
TaqI 267 TCGA T^CGA
TseI 500 GCAGC G^C[AT]GC
TthHB8I 267 TCGA T^CGA
XbaI 355 TCTAGA T^CTAGA
XcmI 493 CCAAACTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 470 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 6 CCGC CCGC(-3/-1)
78 GCGG
AfaI 312 GTAC GT^AC
516 GTAC
BfaI 356 CTAG C^TAG
431 CTAG
BsmFI 3 GTCCC GGGAC(10/14)
17 GTCCC
BsrI 448 ACTGG ACTGG(1/-1)
513 CCAGT
BssKI 62 CCAGG ^CC[ACGT]GG
258 CCAGG
BstNI 62 CCAGG CC^[AT]GG
258 CCAGG
EcoRII 62 CCAGG ^CC[AT]GG
258 CCAGG
Fnu4HI 78 GCGGC GC^[ACGT]GC
500 GCAGC
HgaI 196 GACGC GACGC(5/10)
15 GCGTC
ItaI 78 GCGGC GC^[ACGT]GC
500 GCAGC
MaeI 356 CTAG C^TAG
431 CTAG
MvaI 62 CCAGG CC^[AT]GG
258 CCAGG
RsaI 312 GTAC GT^AC
516 GTAC
ScrFI 62 CCAGG CC^[ACGT]GG
258 CCAGG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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BbsI BpuAI BsaJI DpnII MaeII
MboI MboII MnlI NdeII Sau3AI
SfcI Tsp509I