(Circular) (Six-base) MAPSORT of: pFN21AE0617.seq Check: 3852 from: 1 to: 5254 pFN21AE0617 5254 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3064 278 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2446 2597 1937 Size: 509 151 4594 Fragments arranged by size: 4594 509 151 Acc65I G'GTAC_C Cuts at: 2575 2575 Size: 5254 AclI AA'CG_TT Cuts at: 1563 3381 3754 1563 Size: 1818 373 3063 Fragments arranged by size: 3063 1818 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2217 3317 4701 4969 1632 Size: 462 123 1100 1384 268 1917 Fragments arranged by size: 1917 1384 1100 462 268 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5057 AflIII A'CryG_T Cuts at: 1203 2031 4153 5130 1203 Size: 828 2122 977 1327 Fragments arranged by size: 2122 1327 977 828 AgeI A'CCGG_T Cuts at: 4085 4085 Size: 5254 AhdI GACnn_n'nnGTC Cuts at: 2068 3983 2068 Size: 1915 3339 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5254 AlwNI CAG_nnn'CTG Cuts at: 2997 4569 2997 Size: 1572 3682 ApaLI G'TGCA_C Cuts at: 3311 4467 3311 Size: 1156 4098 ApoI r'AATT_y Cuts at: 1493 2090 2563 2789 2941 5050 1493 Size: 597 473 226 152 2109 1697 Fragments arranged by size: 2109 1697 597 473 226 152 AseI AT'TA_AT Cuts at: 160 1032 3808 160 Size: 872 2776 1606 Fragments arranged by size: 2776 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 AvaI C'yCGr_G Cuts at: 1870 1943 2579 1870 Size: 73 636 4545 Fragments arranged by size: 4545 636 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2341 2374 2341 Size: 33 5221 BamHI G'GATC_C Cuts at: 2584 2951 2584 Size: 367 4887 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2575 4030 618 Size: 325 217 627 788 1455 1842 Fragments arranged by size: 1842 1455 788 627 325 217 BanII G_rGCy'C Cuts at: 1391 2573 1391 Size: 1182 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2082 2205 2464 928 Size: 971 183 123 259 3718 Fragments arranged by size: 3718 971 259 183 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3445 3479 1903 Size: 34 1508 34 3678 Fragments arranged by size: 3678 1508 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3146 4356 3146 Size: 1210 4044 BclI T'GATC_A Cuts at: 1548 1548 Size: 5254 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3865 136 Size: 107 122 71 631 605 2193 1525 Fragments arranged by size: 2193 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5249 5249 Size: 5254 BlpI GC'TnA_GC Cuts at: 2671 2671 Size: 5254 Bme1580I G_kGCm'C Cuts at: 1790 3315 4471 1790 Size: 1525 1156 2573 Fragments arranged by size: 2573 1525 1156 BmgBI CAC'GTC Cuts at: 1318 2034 1318 Size: 716 4538 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2512 2985 3943 Size: 416 53 83 399 24 1095 473 958 Cuts at: 3943 442 Size: 1753 Fragments arranged by size: 1753 1095 958 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5254 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3914 1239 Size: 2675 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3379 4244 4542 3379 Size: 865 298 4091 Fragments arranged by size: 4091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3917 882 Size: 617 2418 2219 Fragments arranged by size: 2418 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3045 493 Size: 711 1841 2702 Fragments arranged by size: 2702 1841 711 BsaBI GATnn'nnATC Cuts at: 5248 5248 Size: 5254 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3061 3443 4078 275 Size: 53 83 186 936 1528 382 635 1451 Fragments arranged by size: 1528 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3687 4085 4359 4506 4917 3687 Size: 398 274 147 411 4024 Fragments arranged by size: 4024 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5029 Fragments arranged by size: 5029 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2398 1186 Size: 1212 4042 BseYI C'CCAG_C Cuts at: 4457 4953 4457 Size: 496 4758 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4681 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2704 3465 4493 1540 Size: 275 186 703 761 1028 2301 Fragments arranged by size: 2301 1028 761 703 275 186 BsiHKAI G_wGCw'C Cuts at: 3315 3400 4471 3315 Size: 85 1071 4098 Fragments arranged by size: 4098 1071 85 BsmI GAATG_Cn' Cuts at: 2765 2858 2765 Size: 93 5161 BsmBI CGTCTCn'nnnn_ Cuts at: 2040 2040 Size: 5254 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2573 3315 3400 4471 1391 Size: 399 783 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 85 BspEI T'CCGG_A Cuts at: 4917 4917 Size: 5254 BspHI T'CATG_A Cuts at: 3143 4873 3143 Size: 1730 3524 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 2609 844 Size: 1181 584 3489 Fragments arranged by size: 3489 1181 584 BsrBI CCG'CTC Cuts at: 1394 1823 3141 1394 Size: 429 1318 3507 Fragments arranged by size: 3507 1318 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3749 3923 65 Size: 1183 2501 174 1396 Fragments arranged by size: 2501 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3898 4085 1700 Size: 110 2088 187 2869 Fragments arranged by size: 2869 2088 187 110 BsrGI T'GTAC_A Cuts at: 96 2316 96 Size: 2220 3034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5254 BssSI C'ACGA_G Cuts at: 3314 4326 3314 Size: 1012 4242 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5254 BstBI TT'CG_AA Cuts at: 4058 4058 Size: 5254 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5254 BstYI r'GATC_y Cuts at: 1972 2584 2951 3336 3353 4805 4891 4903 5249 Size: 612 367 385 17 1452 86 12 346 Cuts at: 5249 1972 Size: 1977 Fragments arranged by size: 1977 1452 612 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2462 513 Size: 552 1397 3305 Fragments arranged by size: 3305 1397 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4515 Fragments arranged by size: 4515 683 56 BtsI GCAGTG_nn' Cuts at: 2770 3564 3584 2770 Size: 794 20 4440 Fragments arranged by size: 4440 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5254 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5219 DraI TTT'AAA Cuts at: 2558 2558 Size: 5254 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4261 809 Size: 507 2945 1802 Fragments arranged by size: 2945 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2701 3590 Size: 54 1425 50 275 18 225 646 889 Cuts at: 3590 5219 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 646 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2701 1537 Size: 275 889 4090 Fragments arranged by size: 4090 889 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3184 1353 Size: 1831 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3841 4215 4361 387 Size: 1064 138 2252 374 146 1280 Fragments arranged by size: 2252 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2217 3317 3914 4701 4969 1239 Size: 393 462 123 1100 597 787 268 1524 Fragments arranged by size: 1524 1100 787 597 462 393 268 123 EcoRI G'AATT_C Cuts at: 2563 2941 2563 Size: 378 4876 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2418 2418 Size: 5254 FspI TGC'GCA Cuts at: 3760 3760 Size: 5254 HaeII r_GCGC'y Cuts at: 1678 4401 1678 Size: 2723 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2269 2301 3895 Size: 32 135 32 482 32 183 32 1594 Cuts at: 3895 3927 3969 4001 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1594 482 183 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2598 2844 669 Size: 988 281 660 246 3079 Fragments arranged by size: 3079 988 660 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5254 HpaI GTT'AAC Cuts at: 2844 2844 Size: 5254 KpnI G_GTAC'C Cuts at: 2579 2579 Size: 5254 MfeI C'AATT_G Cuts at: 2853 2853 Size: 5254 MluI A'CGCG_T Cuts at: 5130 5130 Size: 5254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 4368 4552 1305 Size: 1203 1860 184 2007 Fragments arranged by size: 2007 1860 1203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3432 Fragments arranged by size: 3432 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3212 3571 3730 518 Size: 648 2046 359 159 2042 Fragments arranged by size: 2046 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2508 2670 3347 4495 4740 1606 Size: 902 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 902 677 245 162 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4702 NdeI CA'TA_TG Cuts at: 387 387 Size: 5254 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5254 NotI GC'GGCC_GC Cuts at: 2701 2701 Size: 5254 NspI r_CATG'y Cuts at: 1132 1229 2612 4157 1132 Size: 97 1383 1545 2229 Fragments arranged by size: 2229 1545 1383 97 PasI CC'CwG_GG Cuts at: 1348 2263 1348 Size: 915 4339 PciI A'CATG_T Cuts at: 4153 4153 Size: 5254 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5254 PmeI GTTT'AAAC Cuts at: 2558 2558 Size: 5254 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3323 3355 4836 4868 3323 Size: 32 1481 32 3709 Fragments arranged by size: 3709 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5254 PsiI TTA'TAA Cuts at: 2824 2824 Size: 5254 PstI C_TGCA'G Cuts at: 830 2507 2606 830 Size: 1677 99 3478 Fragments arranged by size: 3478 1677 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5254 PvuII CAG'CTG Cuts at: 2508 2508 Size: 5254 SalI G'TCGA_C Cuts at: 1936 2596 1936 Size: 660 4594 SbfI CC_TGCA'GG Cuts at: 2606 2606 Size: 5254 ScaI AGT'ACT Cuts at: 3502 5214 3502 Size: 1712 3542 SexAI A'CCwGG_T Cuts at: 2066 2066 Size: 5254 SfcI C'TryA_G Cuts at: 826 1045 2109 2416 2503 2602 3737 4418 4609 Size: 219 1064 307 87 99 1135 681 191 Cuts at: 4609 826 Size: 1471 Fragments arranged by size: 1471 1135 1064 681 307 219 191 99 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5254 SmaI CCC'GGG Cuts at: 1872 2581 1872 Size: 709 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3358 4259 4521 820 Size: 197 926 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3045 493 Size: 2552 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5254 SphI G_CATG'C Cuts at: 1132 2612 1132 Size: 1480 3774 SspI AAT'ATT Cuts at: 5 52 3178 5 Size: 47 3126 2081 Fragments arranged by size: 3126 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5254 StyI C'CwwG_G Cuts at: 513 1065 2298 2693 513 Size: 552 1233 395 3074 Fragments arranged by size: 3074 1233 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2388 2464 3281 3298 3451 3636 4081 Size: 1255 808 76 817 17 153 185 445 Cuts at: 4081 325 Size: 1498 Fragments arranged by size: 1498 1255 817 808 445 185 153 76 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 2520 3500 Size: 274 80 33 51 1443 339 204 980 Cuts at: 3500 5212 96 Size: 1712 138 Fragments arranged by size: 1712 1443 980 339 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 2210 2242 5214 5246 2134 Size: 32 44 32 2972 32 2142 Fragments arranged by size: 2972 2142 44 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5254 XbaI T'CTAG_A Cuts at: 2360 2590 2360 Size: 230 5024 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2505 2505 Size: 5254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5254 XmaI C'CCGG_G Cuts at: 1870 2579 1870 Size: 709 4545 XmnI GAAnn'nnTTC Cuts at: 2479 3383 2479 Size: 904 4350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3062 276 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI