Restriction map for
pFN21AE0615
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
Acc65I AccIII AclI AfaI AflII
AflIII AgeI AhdI AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstXI Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MroI MslI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 515 GACGTC GACGT^C
AccI 345 GTATAC GT^[AC][GT]AC
AcsI 274 AAATTC [GA]^AATT[CT]
AcyI 515 GACGTC G[GA]^CG[CT]C
ApoI 274 AAATTC [GA]^AATT[CT]
AvaI 137 CCCGAG C^[CT]CG[GA]G
BalI 405 TGGCCA TGG^CCA
BcnI 190 CCCGG CC^[GC]GG
BglII 15 AGATCT A^GATCT
BpmI 233 CTGGAG CTGGAG(16/14)
BsaHI 515 GACGTC G[GA]^CG[CT]C
BsaWI 509 ACCGGA [AT]^CCGG[AT]
BseRI 474 GAGGAG GAGGAG(10/8)
BsmAI 513 GAGAC GTCTC(1/5)
BsmBI 513 GAGACG CGTCTC(1/5)
BsmI 71 GCATTC GAATGC(1/-1)
BsoBI 137 CCCGAG C^[CT]CG[GA]G
BssSI 22 CACGAG CACGAG(-5/-1)
Bst1107I 345 GTATAC GTA^TAC
BstYI 15 AGATCT [GA]^GATC[CT]
BstZ17I 345 GTATAC GTA^TAC
CfoI 142 GCGC GCG^C
DpnII 16 GATC ^GATC
DraII 133 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 47 CCATGG C^C[GA][CT]GG
EarI 127 GAAGAG CTCTTC(1/4)
EcoO109I 133 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 332 GATATC GAT^ATC
EcoT14I 47 CCATGG C^C[AT][AT]GG
HgaI 491 GACGC GACGC(5/10)
HhaI 142 GCGC GCG^C
Hin1I 515 GACGTC G[GA]^CG[CT]C
HinP1I 142 GCGC G^CGC
HincII 436 GTTGAC GT[CT]^[GA]AC
HindII 436 GTTGAC GT[CT]^[GA]AC
Ksp632I 127 GAAGAG CTCTTC(1/4)
MaeIII 229 GTAAC ^GT[ACGT]AC
MboI 16 GATC ^GATC
MflI 15 AGATCT [GA]^GATC[CT]
MluNI 405 TGGCCA TGG^CCA
MscI 405 TGGCCA TGG^CCA
MseI 296 TTAA T^TAA
NciI 190 CCCGG CC^[GC]GG
NcoI 47 CCATGG C^CATGG
NdeII 16 GATC ^GATC
PpuMI 133 GGGTCCC [GA]G^G[AT]CC[CT]
Sau3AI 16 GATC ^GATC
SfaNI 147 GATGC GCATC(5/9)
StyI 47 CCATGG C^C[AT][AT]GG
TaqI 317 TCGA T^CGA
TfiI 219 GATTC G^A[AT]TC
Tru9I 296 TTAA T^TAA
TthHB8I 317 TCGA T^CGA
XhoII 15 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 112 CGCG CG^CG
143 CGCG
BbsI 259 GAAGAC GAAGAC(2/6)
488 GAAGAC
BbvI 494 GCAGC GCAGC(8/12)
385 GCTGC
BfaI 13 CTAG C^TAG
352 CTAG
BpuAI 259 GAAGAC GAAGAC(2/6)
488 GAAGAC
BsaJI 47 CCATGG C^C[ACGT][ACGT]GG
380 CCCAGG
BsrI 232 ACTGG ACTGG(1/-1)
440 ACTGG
BstNI 381 CCAGG CC^[AT]GG
408 CCAGG
BstUI 112 CGCG CG^CG
143 CGCG
EaeI 405 TGGCCA [CT]^GGCC[GA]
483 TGGCCG
EcoRII 381 CCAGG ^CC[AT]GG
408 CCAGG
HaeIII 406 GGCC GG^CC
484 GGCC
HapII 191 CCGG C^CGG
510 CCGG
HpaII 191 CCGG C^CGG
510 CCGG
MaeI 13 CTAG C^TAG
352 CTAG
MaeII 8 ACGT A^CGT
516 ACGT
MspI 191 CCGG C^CGG
510 CCGG
MvaI 381 CCAGG CC^[AT]GG
408 CCAGG
MvnI 112 CGCG CG^CG
143 CGCG
NlaIII 48 CATG CATG^
241 CATG
PleI 398 GACTC GAGTC(4/5)
236 GAGTC
TseI 385 GCTGC G^C[AT]GC
494 GCAGC
Tsp509I 275 AATT ^AATT
294 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AvaII BsiYI BslI BsmFI
BssKI BstF5I Cfr13I Fnu4HI FokI
HinfI ItaI MboII MnlI MwoI
NlaIV Sau96I ScrFI