Restriction map for
pFN21AE0614
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BclI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 842 ACGTGT A^C[GA][CT]GT
AlwI 972 GATCC GGATC(4/5)
Asp700I 879 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 594 GGCACC G^G[CT][GA]CC
BcgI 81 CGAGTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 750 GGATAC GTATCC(6/5)
BcnI 737 CCCGG CC^[GC]GG
BfaI 838 CTAG C^TAG
BpmI 1061 CTGGAG CTGGAG(16/14)
BsaJI 736 CCCCGG C^C[ACGT][ACGT]GG
BsmFI 159 GGGAC GGGAC(10/14)
BsrBI 1026 CCGCTC CCGCTC(-3/-3)
BstEII 645 GGTGACC G^GT[ACGT]ACC
BstNI 817 CCTGG CC^[AT]GG
BstPI 645 GGTGACC G^GT[ACGT]ACC
BstYI 971 AGATCC [GA]^GATC[CT]
DpnII 972 GATC ^GATC
DraIII 1081 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 366 CCTTCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 645 GGTGACC G^GT[ACGT]ACC
EcoRI 901 GAATTC G^AATTC
EcoRII 817 CCTGG ^CC[AT]GG
FauI 486 GCGGG CCCGC(4/6)
HaeII 955 AGCGCC [GA]GCGC^[CT]
HincII 226 GTCAAC GT[CT]^[GA]AC
HindII 226 GTCAAC GT[CT]^[GA]AC
MaeI 838 CTAG C^TAG
MboI 972 GATC ^GATC
MflI 971 AGATCC [GA]^GATC[CT]
MvaI 817 CCTGG CC^[AT]GG
NciI 737 CCCGG CC^[GC]GG
NdeII 972 GATC ^GATC
NspI 455 GCATGC [GA]CATG^[CT]
PpuMI 327 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 774 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 278 CTGCAG CTGCA^G
Sau3AI 972 GATC ^GATC
SphI 455 GCATGC GCATG^C
TaqI 431 TCGA T^CGA
TthHB8I 431 TCGA T^CGA
XbaI 837 TCTAGA T^CTAGA
XcmI 632 CCATTTTCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 971 AGATCC [GA]^GATC[CT]
XmnI 879 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 880 AAATTC [GA]^AATT[CT]
901 GAATTC
AluI 149 AGCT AG^CT
461 AGCT
ApoI 880 AAATTC [GA]^AATT[CT]
901 GAATTC
AvaII 328 GGACC G^G[AT]CC
733 GGACC
BsmAI 342 GAGAC GTCTC(1/5)
717 GTCTC
BsrDI 87 CATTGC GCAATG(2/0)
1073 GCAATG
BsrFI 482 ACCGGC [GA]^CCGG[CT]
1003 ACCGGC
BssKI 737 CCCGG ^CC[ACGT]GG
817 CCTGG
BstF5I 21 CATCC GGATG(2/0)
473 GGATG
CfoI 956 GCGC GCG^C
987 GCGC
Cfr10I 482 ACCGGC [GA]^CCGG[CT]
1003 ACCGGC
DdeI 618 CTTAG C^T[ACGT]AG
1020 CTCAG
FokI 21 CATCC GGATG(9/13)
473 GGATG
HhaI 956 GCGC GCG^C
987 GCGC
HinP1I 956 GCGC G^CGC
987 GCGC
HphI 153 GGTGA GGTGA(8/7)
645 GGTGA
MaeII 842 ACGT A^CGT
863 ACGT
PflMI 125 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1080 CCACTCTGTGG
ScrFI 737 CCCGG CC^[ACGT]GG
817 CCTGG
SfaNI 117 GCATC GCATC(5/9)
400 GCATC
SfcI 278 CTGCAG C^T[GA][CT]AG
603 CTACAG
TfiI 167 GAATC G^A[AT]TC
744 GAATC
Van91I 125 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1080 CCACTCTGTGG
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BalI BbsI BbvI BpuAI
BsiYI BslI BsrI BstXI Cac8I
Cfr13I DraII EaeI EcoO109I Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboII MluNI MnlI MscI
MslI MspI MwoI NlaIII NlaIV
PleI Sau96I TseI Tsp45I Tsp509I
TspRI